Evaluation of hepatoprotective effect of aqueous extract of Schrebera swietenioides bark against carbon tetrachloride-induced toxicity in wistar rats

Nitesh Kumar, Anil Choudhary, Amit Tiwari, Amol Rasal, Raghu Jetti, C. Mallikarjuna Rao

Research output: Contribution to journalArticle

Abstract

The present study was designed to explore the hepatoprotective action of aqueous extract of stem bark of Schrebera swietenioides against carbon tetrachloride (CCl4) induced hepatotoxicity in Wistar rats. Rats were divided in four groups viz., sham, toxicant control, standard (silymarin, 50 mg/kg, p.o.) and SS extract (100 mg/kg, p.o.) group. Treatment was continued for 7 days. On the 7th day, CCl4 was injected intraperitoneally (0.5 ml/kg in olive oil) to every animal except sham animals. On the 8th day, blood was withdrawn, serum was separated and analysis of liver function tests such as AST, ALT, ALP and total bilirubin were performed. Rats were sacrificed and liver was isolated from each rat. Part of the liver was homogenized and used for determination of antioxidant parameters, namely catalase, SOD, GSH, GST, total thiols and lipid peroxidation. Remaining part were subjected to histopathology. The Schrebera swietenioides extract treatment showed a significant (P <0_05) reduction in the levels of AST, ALT and total bilirubin levels and significant rise in catalase, SOD and GSH levels compared to the toxicant control group. Histopathological investigation supported the hepatoptotective potential of Schrebera swietenioides extract by minimizing fatty accumulation and necrosis events in the liver architecture compared to the toxicant control group. These results identify the ability of extract to be a potent hepatoptotective agent which were comparable to silymarin.

Original languageEnglish
Pages (from-to)1917-1920
Number of pages4
JournalAdvanced Science Letters
Volume23
Issue number3
DOIs
Publication statusPublished - 01-03-2017

Fingerprint

Carbon tetrachloride
Carbon Tetrachloride
Toxicants
Toxicity
Liver
bark
Silymarin
Wistar Rats
Rats
Carbon
toxicity
Superoxide Dismutase
Bilirubin
Catalase
histopathology
animal
thiol
carbon
Evaluation
evaluation

All Science Journal Classification (ASJC) codes

  • Computer Science(all)
  • Health(social science)
  • Mathematics(all)
  • Education
  • Environmental Science(all)
  • Engineering(all)
  • Energy(all)

Cite this

@article{b42380190ad745eaaf3fece8ba0b66f5,
title = "Evaluation of hepatoprotective effect of aqueous extract of Schrebera swietenioides bark against carbon tetrachloride-induced toxicity in wistar rats",
abstract = "The present study was designed to explore the hepatoprotective action of aqueous extract of stem bark of Schrebera swietenioides against carbon tetrachloride (CCl4) induced hepatotoxicity in Wistar rats. Rats were divided in four groups viz., sham, toxicant control, standard (silymarin, 50 mg/kg, p.o.) and SS extract (100 mg/kg, p.o.) group. Treatment was continued for 7 days. On the 7th day, CCl4 was injected intraperitoneally (0.5 ml/kg in olive oil) to every animal except sham animals. On the 8th day, blood was withdrawn, serum was separated and analysis of liver function tests such as AST, ALT, ALP and total bilirubin were performed. Rats were sacrificed and liver was isolated from each rat. Part of the liver was homogenized and used for determination of antioxidant parameters, namely catalase, SOD, GSH, GST, total thiols and lipid peroxidation. Remaining part were subjected to histopathology. The Schrebera swietenioides extract treatment showed a significant (P <0_05) reduction in the levels of AST, ALT and total bilirubin levels and significant rise in catalase, SOD and GSH levels compared to the toxicant control group. Histopathological investigation supported the hepatoptotective potential of Schrebera swietenioides extract by minimizing fatty accumulation and necrosis events in the liver architecture compared to the toxicant control group. These results identify the ability of extract to be a potent hepatoptotective agent which were comparable to silymarin.",
author = "Nitesh Kumar and Anil Choudhary and Amit Tiwari and Amol Rasal and Raghu Jetti and {Mallikarjuna Rao}, C.",
year = "2017",
month = "3",
day = "1",
doi = "10.1166/asl.2017.8539",
language = "English",
volume = "23",
pages = "1917--1920",
journal = "Advanced Science Letters",
issn = "1936-6612",
publisher = "American Scientific Publishers",
number = "3",

}

Evaluation of hepatoprotective effect of aqueous extract of Schrebera swietenioides bark against carbon tetrachloride-induced toxicity in wistar rats. / Kumar, Nitesh; Choudhary, Anil; Tiwari, Amit; Rasal, Amol; Jetti, Raghu; Mallikarjuna Rao, C.

In: Advanced Science Letters, Vol. 23, No. 3, 01.03.2017, p. 1917-1920.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Evaluation of hepatoprotective effect of aqueous extract of Schrebera swietenioides bark against carbon tetrachloride-induced toxicity in wistar rats

AU - Kumar, Nitesh

AU - Choudhary, Anil

AU - Tiwari, Amit

AU - Rasal, Amol

AU - Jetti, Raghu

AU - Mallikarjuna Rao, C.

PY - 2017/3/1

Y1 - 2017/3/1

N2 - The present study was designed to explore the hepatoprotective action of aqueous extract of stem bark of Schrebera swietenioides against carbon tetrachloride (CCl4) induced hepatotoxicity in Wistar rats. Rats were divided in four groups viz., sham, toxicant control, standard (silymarin, 50 mg/kg, p.o.) and SS extract (100 mg/kg, p.o.) group. Treatment was continued for 7 days. On the 7th day, CCl4 was injected intraperitoneally (0.5 ml/kg in olive oil) to every animal except sham animals. On the 8th day, blood was withdrawn, serum was separated and analysis of liver function tests such as AST, ALT, ALP and total bilirubin were performed. Rats were sacrificed and liver was isolated from each rat. Part of the liver was homogenized and used for determination of antioxidant parameters, namely catalase, SOD, GSH, GST, total thiols and lipid peroxidation. Remaining part were subjected to histopathology. The Schrebera swietenioides extract treatment showed a significant (P <0_05) reduction in the levels of AST, ALT and total bilirubin levels and significant rise in catalase, SOD and GSH levels compared to the toxicant control group. Histopathological investigation supported the hepatoptotective potential of Schrebera swietenioides extract by minimizing fatty accumulation and necrosis events in the liver architecture compared to the toxicant control group. These results identify the ability of extract to be a potent hepatoptotective agent which were comparable to silymarin.

AB - The present study was designed to explore the hepatoprotective action of aqueous extract of stem bark of Schrebera swietenioides against carbon tetrachloride (CCl4) induced hepatotoxicity in Wistar rats. Rats were divided in four groups viz., sham, toxicant control, standard (silymarin, 50 mg/kg, p.o.) and SS extract (100 mg/kg, p.o.) group. Treatment was continued for 7 days. On the 7th day, CCl4 was injected intraperitoneally (0.5 ml/kg in olive oil) to every animal except sham animals. On the 8th day, blood was withdrawn, serum was separated and analysis of liver function tests such as AST, ALT, ALP and total bilirubin were performed. Rats were sacrificed and liver was isolated from each rat. Part of the liver was homogenized and used for determination of antioxidant parameters, namely catalase, SOD, GSH, GST, total thiols and lipid peroxidation. Remaining part were subjected to histopathology. The Schrebera swietenioides extract treatment showed a significant (P <0_05) reduction in the levels of AST, ALT and total bilirubin levels and significant rise in catalase, SOD and GSH levels compared to the toxicant control group. Histopathological investigation supported the hepatoptotective potential of Schrebera swietenioides extract by minimizing fatty accumulation and necrosis events in the liver architecture compared to the toxicant control group. These results identify the ability of extract to be a potent hepatoptotective agent which were comparable to silymarin.

UR - http://www.scopus.com/inward/record.url?scp=85018613316&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85018613316&partnerID=8YFLogxK

U2 - 10.1166/asl.2017.8539

DO - 10.1166/asl.2017.8539

M3 - Article

VL - 23

SP - 1917

EP - 1920

JO - Advanced Science Letters

JF - Advanced Science Letters

SN - 1936-6612

IS - 3

ER -