Evaluation of in vitro and in vivo anticancer potential of two 5-acetamido chalcones against breast cancer

Sonal Wankhede, Nitesh Kumar, Lalitha Simon, Subhankar Biswas, Karthik Gourishetti, Grandhi Venkata Ramalingayya, Mit Joshi, C. Mallikarjuna Rao

Research output: Contribution to journalArticle

Abstract

Two 5’acetamido chalcones, C1 and C2 were synthesized by Claisen-Schmidt condensation method and characterized by IR, LC-MS, 1H NMR and 13C NMR. These compounds were evaluated for anticancer activity in vitro in breast cancer cell lines (MCF-7 and MDA-MB-231) using MTT assay, anti-metastatic assay, apoptotic screening by AO/EB staining and in vivo in N-Methyl-N-nitrosourea (MNU) induced breast carcinoma model. Sprague-Dawley rats with developed tumors (50 mg/kg MNU i.p.) were grouped in four, namely MNU control (0.25 % of CMC p.o.), standard group (doxorubicin 2 mg/kg once in 4 days, i.p.), C1 and C2 groups (50 mg/kg p.o. each). After 21 days of treatments, tumor volume and weight were assessed. Excised tumors were subjected to DNA fragmentation study. MTT assay showed IC50 values of 62.56 and 37.8 μM by for C1 and C2. Both compounds increased apoptotic bodies more than 3 fold compared to normal control in AO/EB staining. Antimetastatic (scratch wound) assay showed a significant (p<0.05) reduction in cell migration after 24 h and 48 h treatments compared to normal control. In in vivo studies, tumor weight and tumor volume were significantly (p<0.05) reduced in the doxorubicin group as well as in test groups compared to MNU control. DNA fragmentation assay showed an increase in the number of bands formed in C1 and C2 compared to normal control. Results obtained from in vitro and in vivo studies demonstrated the significant anticancer potentials of C1 and C2.

Original languageEnglish
Pages (from-to)1150-1163
Number of pages14
JournalEXCLI Journal
Volume16
DOIs
Publication statusPublished - 19-10-2017

Fingerprint

Chalcones
chalcones
Tumor Burden
breast neoplasms
Breast Neoplasms
neoplasms
assays
DNA Fragmentation
doxorubicin
Doxorubicin
DNA fragmentation
in vivo studies
Staining and Labeling
Methylnitrosourea
N-methyl-N-nitrosourea
Inhibitory Concentration 50
Cell Movement
Sprague Dawley Rats
plant damage
Neoplasms

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Animal Science and Zoology
  • Pharmacology
  • Drug Discovery

Cite this

Wankhede, Sonal ; Kumar, Nitesh ; Simon, Lalitha ; Biswas, Subhankar ; Gourishetti, Karthik ; Ramalingayya, Grandhi Venkata ; Joshi, Mit ; Rao, C. Mallikarjuna. / Evaluation of in vitro and in vivo anticancer potential of two 5-acetamido chalcones against breast cancer. In: EXCLI Journal. 2017 ; Vol. 16. pp. 1150-1163.
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Evaluation of in vitro and in vivo anticancer potential of two 5-acetamido chalcones against breast cancer. / Wankhede, Sonal; Kumar, Nitesh; Simon, Lalitha; Biswas, Subhankar; Gourishetti, Karthik; Ramalingayya, Grandhi Venkata; Joshi, Mit; Rao, C. Mallikarjuna.

In: EXCLI Journal, Vol. 16, 19.10.2017, p. 1150-1163.

Research output: Contribution to journalArticle

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AU - Wankhede, Sonal

AU - Kumar, Nitesh

AU - Simon, Lalitha

AU - Biswas, Subhankar

AU - Gourishetti, Karthik

AU - Ramalingayya, Grandhi Venkata

AU - Joshi, Mit

AU - Rao, C. Mallikarjuna

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