Evaluation of p53 protein expression in Barrett esophagus

Mahathi Krothapalli, Jyoti Ramnath Kini, Hema Kini, Kausalya Kumari Sahu, Suresh Shenoy, Sandeep Gopal Krishna, B. V. Tantry

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: Loss of heterozygosity of p53 along with aneuploidy is deemed to be the early molecular steps in Barrett metaplasia-dysplasia-adenocarcinoma sequence. Objective biomarkers need to be used along with microscopy for risk stratification to predict the progression of Barrett esophagus (BE) to carcinoma. Aim: This study aims to study p53 protein expression in dysplasia and correlate the same with morphology in BE. Materials and Methods: A time-bound study was conducted from January 2011 to June 2015. All esophageal biopsies showing histological evidence of columnar epithelium with the presence of goblet cells were included. The cases which showed dysplasia were graded on hematoxylin and eosin stain. Evaluation of p53 immunohistochemistry staining was done on all the cases of BE. Dysplasia was correlated with the expression of p53 using Chi-square value (χ2) and Fischer's exact test wherever appropriate. P < 0.05 was considered to be statistically significant. Results: Of 829 esophageal biopsies received, 119 were endoscopically suspected to be BE, of which 85 cases were confirmed on microscopy. In our study, there were 75 cases negative for dysplasia (88.2%), 8 with low-grade dysplasia (LGD) (9.4%), and two with high-grade dysplasia (HGD) (2.4%). Three cases of BE had associated adenocarcinoma. Immunostaining with p53 done on all the 85 cases showed positive staining in all cases with LGD, one with HGD and two with adenocarcinoma. In the present study, immunostaining with p53 showed 90% sensitivity, 89.3% specificity, positive predictive value of 52.9%, and negative predictive value of 98.5%. Conclusion: The technical simplicity, easy availability, and comparatively lower cost enhance the role of p53 as a biomarker in risk stratification for patients with BE.

Original languageEnglish
Pages (from-to)170-175
Number of pages6
JournalIndian Journal of Pathology and Microbiology
Volume61
Issue number2
DOIs
Publication statusPublished - 01-04-2018

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Barrett Esophagus
Proteins
Adenocarcinoma
Microscopy
Biomarkers
Staining and Labeling
Biopsy
Goblet Cells
Loss of Heterozygosity
Aneuploidy
Hematoxylin
Eosine Yellowish-(YS)
Coloring Agents
Epithelium
Immunohistochemistry
Carcinoma
Costs and Cost Analysis
Sensitivity and Specificity

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Microbiology (medical)

Cite this

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title = "Evaluation of p53 protein expression in Barrett esophagus",
abstract = "Background: Loss of heterozygosity of p53 along with aneuploidy is deemed to be the early molecular steps in Barrett metaplasia-dysplasia-adenocarcinoma sequence. Objective biomarkers need to be used along with microscopy for risk stratification to predict the progression of Barrett esophagus (BE) to carcinoma. Aim: This study aims to study p53 protein expression in dysplasia and correlate the same with morphology in BE. Materials and Methods: A time-bound study was conducted from January 2011 to June 2015. All esophageal biopsies showing histological evidence of columnar epithelium with the presence of goblet cells were included. The cases which showed dysplasia were graded on hematoxylin and eosin stain. Evaluation of p53 immunohistochemistry staining was done on all the cases of BE. Dysplasia was correlated with the expression of p53 using Chi-square value (χ2) and Fischer's exact test wherever appropriate. P < 0.05 was considered to be statistically significant. Results: Of 829 esophageal biopsies received, 119 were endoscopically suspected to be BE, of which 85 cases were confirmed on microscopy. In our study, there were 75 cases negative for dysplasia (88.2{\%}), 8 with low-grade dysplasia (LGD) (9.4{\%}), and two with high-grade dysplasia (HGD) (2.4{\%}). Three cases of BE had associated adenocarcinoma. Immunostaining with p53 done on all the 85 cases showed positive staining in all cases with LGD, one with HGD and two with adenocarcinoma. In the present study, immunostaining with p53 showed 90{\%} sensitivity, 89.3{\%} specificity, positive predictive value of 52.9{\%}, and negative predictive value of 98.5{\%}. Conclusion: The technical simplicity, easy availability, and comparatively lower cost enhance the role of p53 as a biomarker in risk stratification for patients with BE.",
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Evaluation of p53 protein expression in Barrett esophagus. / Krothapalli, Mahathi; Kini, Jyoti Ramnath; Kini, Hema; Sahu, Kausalya Kumari; Shenoy, Suresh; Krishna, Sandeep Gopal; Tantry, B. V.

In: Indian Journal of Pathology and Microbiology, Vol. 61, No. 2, 01.04.2018, p. 170-175.

Research output: Contribution to journalArticle

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T1 - Evaluation of p53 protein expression in Barrett esophagus

AU - Krothapalli, Mahathi

AU - Kini, Jyoti Ramnath

AU - Kini, Hema

AU - Sahu, Kausalya Kumari

AU - Shenoy, Suresh

AU - Krishna, Sandeep Gopal

AU - Tantry, B. V.

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