Evaluation of saponin rich fraction of Trigonella foenum graecum for antihypertensive activity

A.T. Sudheendra, R. Shenoy, A.D. Taranalli

Research output: Contribution to journalArticle

Abstract

To separate the saponin rich fraction of Trigonella foenum graecum (TFG) seeds and to evaluate its influence on blood pressure in normal and hypertensive rats. The ethanolic extract of TFG seeds was fractionated with cold diethyl ether to obtain saponin rich fraction. Albino rats of Wistar strain of either sex weighing between 200 to 250 g were used for the hypertension models. Hypertension was induced by 1K-1C Goldblatt method. Systolic BP was measured by Harward non invasive BP apparatus and Biopack data acquisition system. The treatment was given as a single dose at two different dose levels of 100 mg/kg and 200 mg/kg bodyweight. The saponin rich fraction of TFG seeds showed significant, dose dependent antihypertensive activity. The extract did not show any observable decrease in blood pressure in normal non hypertensive rats. The results demonstrate the effectiveness of TFG saponins as a potential treatment for hypertension.
Original languageEnglish
Pages (from-to)1229-1233
Number of pages5
JournalPharmacologyonline
Volume1
Publication statusPublished - 2009

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Trigonella
Saponins
Antihypertensive Agents
Seeds
Hypertension
Blood Pressure
Information Systems
Ether
Wistar Rats

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@article{6271ababca6645a5a58f3593226aff57,
title = "Evaluation of saponin rich fraction of Trigonella foenum graecum for antihypertensive activity",
abstract = "To separate the saponin rich fraction of Trigonella foenum graecum (TFG) seeds and to evaluate its influence on blood pressure in normal and hypertensive rats. The ethanolic extract of TFG seeds was fractionated with cold diethyl ether to obtain saponin rich fraction. Albino rats of Wistar strain of either sex weighing between 200 to 250 g were used for the hypertension models. Hypertension was induced by 1K-1C Goldblatt method. Systolic BP was measured by Harward non invasive BP apparatus and Biopack data acquisition system. The treatment was given as a single dose at two different dose levels of 100 mg/kg and 200 mg/kg bodyweight. The saponin rich fraction of TFG seeds showed significant, dose dependent antihypertensive activity. The extract did not show any observable decrease in blood pressure in normal non hypertensive rats. The results demonstrate the effectiveness of TFG saponins as a potential treatment for hypertension.",
author = "A.T. Sudheendra and R. Shenoy and A.D. Taranalli",
note = "Export Date: 10 November 2017 Correspondence Address: Sudheendra, A.T.; Dept. of Pharmacology, MCOPS, Manipal - 576 104, India; email: arun.ts@manipal.edu Chemicals/CAS: enalapril maleate, 76095-16-4; ether, 60-29-7; methanol, 67-56-1; saponin, 8047-15-2 References: Patricia, M.K., Megan, W., Kristi, R., Paul, M., Paul, K.W., Jiang, H., Global burden of hypertension: Analysis of worldwide data (2005) Lancet, 365, pp. 217-223; Matthew, E.D., Curt, D.S., Genetic Basis of Hypertension. Revisiting Angiotensinogen (2006) Hypertension, 48, pp. 14-20; Ahmad VU, Noorwala M, Mohammad FV, Sener B, Gilani AH, Aftab K. Symphytoxide A, a triterpenoid saponin from the roots of Symphytum officinale. Phytochemistry 1993; 32: 1003-1006; Sagesaka-Mitane, Y., Sugiura, T., Miwa, Y., Yamaguchi, K., Kyuki, K., Effect of tea-leaf saponin on blood pressure of spontaneously hypertensive rats (1996) Yakugaku Zasshi, 116, pp. 388-395; Jeon, B.H., Kim, C.S., Kim, H.S., Park, J.B., Nam, K.Y., Chang, S.J., Effect of Korean red ginseng on blood pressure and nitric oxide production (2000) Acta Pharmacologica Sinica, 21, pp. 1095-1100; Rhiouani, H., Lyoussi, B., Settaf, A., Cherrah, Y., Hassar, M., Antihypertensive effect of Herniaria glabra saponins in the spontaneously hypertensive rat (2001) Annales Pharmaceutiques Francaises, 59, pp. 211-214; Gerhard Vogel, H., (2002) Drug Discovery and evaluation; Pharmacological assays, pp. 172-173. , 2nd Edition, Springer-Verlag Berlin Heidelberg; George F, Zohar K, Harinder P S, Makkar, Klaus B. The biological action of saponins in animal systems: a review. Br J Nutr 2002; 88: 587-605UR - https://www.scopus.com/inward/record.uri?eid=2-s2.0-66249145761&partnerID=40&md5=9faf8f7cb9f5d1d85c93df6ddfff87b0",
year = "2009",
language = "English",
volume = "1",
pages = "1229--1233",
journal = "Pharmacologyonline",
issn = "1827-8620",
publisher = "SILAE (Italo-Latin American Society of Ethnomedicine)",

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Evaluation of saponin rich fraction of Trigonella foenum graecum for antihypertensive activity. / Sudheendra, A.T.; Shenoy, R.; Taranalli, A.D.

In: Pharmacologyonline, Vol. 1, 2009, p. 1229-1233.

Research output: Contribution to journalArticle

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T1 - Evaluation of saponin rich fraction of Trigonella foenum graecum for antihypertensive activity

AU - Sudheendra, A.T.

AU - Shenoy, R.

AU - Taranalli, A.D.

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N2 - To separate the saponin rich fraction of Trigonella foenum graecum (TFG) seeds and to evaluate its influence on blood pressure in normal and hypertensive rats. The ethanolic extract of TFG seeds was fractionated with cold diethyl ether to obtain saponin rich fraction. Albino rats of Wistar strain of either sex weighing between 200 to 250 g were used for the hypertension models. Hypertension was induced by 1K-1C Goldblatt method. Systolic BP was measured by Harward non invasive BP apparatus and Biopack data acquisition system. The treatment was given as a single dose at two different dose levels of 100 mg/kg and 200 mg/kg bodyweight. The saponin rich fraction of TFG seeds showed significant, dose dependent antihypertensive activity. The extract did not show any observable decrease in blood pressure in normal non hypertensive rats. The results demonstrate the effectiveness of TFG saponins as a potential treatment for hypertension.

AB - To separate the saponin rich fraction of Trigonella foenum graecum (TFG) seeds and to evaluate its influence on blood pressure in normal and hypertensive rats. The ethanolic extract of TFG seeds was fractionated with cold diethyl ether to obtain saponin rich fraction. Albino rats of Wistar strain of either sex weighing between 200 to 250 g were used for the hypertension models. Hypertension was induced by 1K-1C Goldblatt method. Systolic BP was measured by Harward non invasive BP apparatus and Biopack data acquisition system. The treatment was given as a single dose at two different dose levels of 100 mg/kg and 200 mg/kg bodyweight. The saponin rich fraction of TFG seeds showed significant, dose dependent antihypertensive activity. The extract did not show any observable decrease in blood pressure in normal non hypertensive rats. The results demonstrate the effectiveness of TFG saponins as a potential treatment for hypertension.

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JO - Pharmacologyonline

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SN - 1827-8620

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