Evaluation of the aphrodisiac potential of a chemically characterized aqueous extract of Tamarindus indica pulp

Amita Rai, Snehashis Das, Mallikarjuna Rao Chamallamudi, Krishnadas Nandakumar, Raghavendra Shetty, Meghna Gill, Suhani Sumalatha, Raviraj Devkar, Karthik Gourishetti, Nitesh Kumar

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Ethnopharmacological relevance Tamarindus indica is an ingredient in the traditional aphrodisiac formulations in Africa and India. It is also a widely used food ingredient in other tropical countries. Aim of the study The present study was aimed to evaluate the aphrodisiac potential and reproductive safety profile of aqueous extract of Tamarindus indica in male Wistar rats. Materials and methods The aqueous extract was prepared by maceration of pulp followed by reduction of volume in rotavapor under heat followed by freeze drying. The prepared extract was characterized for contents of total phenol, flavonoid, and saponin. It was also subjected to phytoconstituent analysis using GCMS. Further, the extract was evaluated for acute toxicity study. The aphrodisiac and reproductive toxicity potential were evaluated in animals after grouping them in four with six animals each namely, normal control, standard (Sildenafil citrate, 4 mg/kg p.o.) and extract of Tamarindus indica treated groups at two dose levels, 125 and 250 mg/kg p.o. The study was conducted for 54 days with daily once dosing of extract and standard. Equal number of females was grouped without treatment for evaluation of parameters of sexual desire (mount frequency and intromission frequency) and parameters of sexual arousal (mount latency and intromission latency). These parameters were evaluated on day 14, 28, 42 and 54. Animals were sacrificed on day 54, testes were removed and studied for histopathological changes. Results The extract showed 6.6 mg gallic acid equivalent/g of total phenol, 2.3 mg catechin equivalent/g of flavonoid and 11.6% saponin. Forty chemical constituents were identified by GCMS analysis. In acute toxicity study, the extract was found to be safe till 2000 mg/kg p.o. Efficacy study showed significant (p<0.05) improvement in parameters of sexual desire (mount frequency and intromission frequency) and parameters of sexual arousal on all observed days except mount frequency for 125 mg/kg on 42nd day and intromission frequency for both doses of tamarind compared to normal control. Improvements in these parameters were comparable to the standard drug. Histopathology study and sperm count suggested an increase in sperm production without any sign of toxicity in testis. Sperm motility significantly (p<0.05) increased in the treatment groups that received extract at 250 mg/kg compared to normal control. Conclusion Aqueous extract of Tamarindus indica possessed aphrodisiac activity together with spermatogenic potential.

Original languageEnglish
Pages (from-to)118-124
Number of pages7
JournalJournal of Ethnopharmacology
Volume210
DOIs
Publication statusPublished - 10-01-2018

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Tamarindus
Aphrodisiacs
Saponins
Phenol
Arousal
Flavonoids
Gas Chromatography-Mass Spectrometry
Testis
Gallic Acid
Sperm Count
Freeze Drying
Catechin
Sperm Motility
Spermatozoa
Wistar Rats
India
Hot Temperature
Safety
Food
Therapeutics

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Drug Discovery

Cite this

@article{18bb1dcc74544c43a92b59b7c4550482,
title = "Evaluation of the aphrodisiac potential of a chemically characterized aqueous extract of Tamarindus indica pulp",
abstract = "Ethnopharmacological relevance Tamarindus indica is an ingredient in the traditional aphrodisiac formulations in Africa and India. It is also a widely used food ingredient in other tropical countries. Aim of the study The present study was aimed to evaluate the aphrodisiac potential and reproductive safety profile of aqueous extract of Tamarindus indica in male Wistar rats. Materials and methods The aqueous extract was prepared by maceration of pulp followed by reduction of volume in rotavapor under heat followed by freeze drying. The prepared extract was characterized for contents of total phenol, flavonoid, and saponin. It was also subjected to phytoconstituent analysis using GCMS. Further, the extract was evaluated for acute toxicity study. The aphrodisiac and reproductive toxicity potential were evaluated in animals after grouping them in four with six animals each namely, normal control, standard (Sildenafil citrate, 4 mg/kg p.o.) and extract of Tamarindus indica treated groups at two dose levels, 125 and 250 mg/kg p.o. The study was conducted for 54 days with daily once dosing of extract and standard. Equal number of females was grouped without treatment for evaluation of parameters of sexual desire (mount frequency and intromission frequency) and parameters of sexual arousal (mount latency and intromission latency). These parameters were evaluated on day 14, 28, 42 and 54. Animals were sacrificed on day 54, testes were removed and studied for histopathological changes. Results The extract showed 6.6 mg gallic acid equivalent/g of total phenol, 2.3 mg catechin equivalent/g of flavonoid and 11.6{\%} saponin. Forty chemical constituents were identified by GCMS analysis. In acute toxicity study, the extract was found to be safe till 2000 mg/kg p.o. Efficacy study showed significant (p<0.05) improvement in parameters of sexual desire (mount frequency and intromission frequency) and parameters of sexual arousal on all observed days except mount frequency for 125 mg/kg on 42nd day and intromission frequency for both doses of tamarind compared to normal control. Improvements in these parameters were comparable to the standard drug. Histopathology study and sperm count suggested an increase in sperm production without any sign of toxicity in testis. Sperm motility significantly (p<0.05) increased in the treatment groups that received extract at 250 mg/kg compared to normal control. Conclusion Aqueous extract of Tamarindus indica possessed aphrodisiac activity together with spermatogenic potential.",
author = "Amita Rai and Snehashis Das and Chamallamudi, {Mallikarjuna Rao} and Krishnadas Nandakumar and Raghavendra Shetty and Meghna Gill and Suhani Sumalatha and Raviraj Devkar and Karthik Gourishetti and Nitesh Kumar",
year = "2018",
month = "1",
day = "10",
doi = "10.1016/j.jep.2017.08.016",
language = "English",
volume = "210",
pages = "118--124",
journal = "Journal of Ethnopharmacology",
issn = "0378-8741",
publisher = "Elsevier Ireland Ltd",

}

Evaluation of the aphrodisiac potential of a chemically characterized aqueous extract of Tamarindus indica pulp. / Rai, Amita; Das, Snehashis; Chamallamudi, Mallikarjuna Rao; Nandakumar, Krishnadas; Shetty, Raghavendra; Gill, Meghna; Sumalatha, Suhani; Devkar, Raviraj; Gourishetti, Karthik; Kumar, Nitesh.

In: Journal of Ethnopharmacology, Vol. 210, 10.01.2018, p. 118-124.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Evaluation of the aphrodisiac potential of a chemically characterized aqueous extract of Tamarindus indica pulp

AU - Rai, Amita

AU - Das, Snehashis

AU - Chamallamudi, Mallikarjuna Rao

AU - Nandakumar, Krishnadas

AU - Shetty, Raghavendra

AU - Gill, Meghna

AU - Sumalatha, Suhani

AU - Devkar, Raviraj

AU - Gourishetti, Karthik

AU - Kumar, Nitesh

PY - 2018/1/10

Y1 - 2018/1/10

N2 - Ethnopharmacological relevance Tamarindus indica is an ingredient in the traditional aphrodisiac formulations in Africa and India. It is also a widely used food ingredient in other tropical countries. Aim of the study The present study was aimed to evaluate the aphrodisiac potential and reproductive safety profile of aqueous extract of Tamarindus indica in male Wistar rats. Materials and methods The aqueous extract was prepared by maceration of pulp followed by reduction of volume in rotavapor under heat followed by freeze drying. The prepared extract was characterized for contents of total phenol, flavonoid, and saponin. It was also subjected to phytoconstituent analysis using GCMS. Further, the extract was evaluated for acute toxicity study. The aphrodisiac and reproductive toxicity potential were evaluated in animals after grouping them in four with six animals each namely, normal control, standard (Sildenafil citrate, 4 mg/kg p.o.) and extract of Tamarindus indica treated groups at two dose levels, 125 and 250 mg/kg p.o. The study was conducted for 54 days with daily once dosing of extract and standard. Equal number of females was grouped without treatment for evaluation of parameters of sexual desire (mount frequency and intromission frequency) and parameters of sexual arousal (mount latency and intromission latency). These parameters were evaluated on day 14, 28, 42 and 54. Animals were sacrificed on day 54, testes were removed and studied for histopathological changes. Results The extract showed 6.6 mg gallic acid equivalent/g of total phenol, 2.3 mg catechin equivalent/g of flavonoid and 11.6% saponin. Forty chemical constituents were identified by GCMS analysis. In acute toxicity study, the extract was found to be safe till 2000 mg/kg p.o. Efficacy study showed significant (p<0.05) improvement in parameters of sexual desire (mount frequency and intromission frequency) and parameters of sexual arousal on all observed days except mount frequency for 125 mg/kg on 42nd day and intromission frequency for both doses of tamarind compared to normal control. Improvements in these parameters were comparable to the standard drug. Histopathology study and sperm count suggested an increase in sperm production without any sign of toxicity in testis. Sperm motility significantly (p<0.05) increased in the treatment groups that received extract at 250 mg/kg compared to normal control. Conclusion Aqueous extract of Tamarindus indica possessed aphrodisiac activity together with spermatogenic potential.

AB - Ethnopharmacological relevance Tamarindus indica is an ingredient in the traditional aphrodisiac formulations in Africa and India. It is also a widely used food ingredient in other tropical countries. Aim of the study The present study was aimed to evaluate the aphrodisiac potential and reproductive safety profile of aqueous extract of Tamarindus indica in male Wistar rats. Materials and methods The aqueous extract was prepared by maceration of pulp followed by reduction of volume in rotavapor under heat followed by freeze drying. The prepared extract was characterized for contents of total phenol, flavonoid, and saponin. It was also subjected to phytoconstituent analysis using GCMS. Further, the extract was evaluated for acute toxicity study. The aphrodisiac and reproductive toxicity potential were evaluated in animals after grouping them in four with six animals each namely, normal control, standard (Sildenafil citrate, 4 mg/kg p.o.) and extract of Tamarindus indica treated groups at two dose levels, 125 and 250 mg/kg p.o. The study was conducted for 54 days with daily once dosing of extract and standard. Equal number of females was grouped without treatment for evaluation of parameters of sexual desire (mount frequency and intromission frequency) and parameters of sexual arousal (mount latency and intromission latency). These parameters were evaluated on day 14, 28, 42 and 54. Animals were sacrificed on day 54, testes were removed and studied for histopathological changes. Results The extract showed 6.6 mg gallic acid equivalent/g of total phenol, 2.3 mg catechin equivalent/g of flavonoid and 11.6% saponin. Forty chemical constituents were identified by GCMS analysis. In acute toxicity study, the extract was found to be safe till 2000 mg/kg p.o. Efficacy study showed significant (p<0.05) improvement in parameters of sexual desire (mount frequency and intromission frequency) and parameters of sexual arousal on all observed days except mount frequency for 125 mg/kg on 42nd day and intromission frequency for both doses of tamarind compared to normal control. Improvements in these parameters were comparable to the standard drug. Histopathology study and sperm count suggested an increase in sperm production without any sign of toxicity in testis. Sperm motility significantly (p<0.05) increased in the treatment groups that received extract at 250 mg/kg compared to normal control. Conclusion Aqueous extract of Tamarindus indica possessed aphrodisiac activity together with spermatogenic potential.

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U2 - 10.1016/j.jep.2017.08.016

DO - 10.1016/j.jep.2017.08.016

M3 - Article

VL - 210

SP - 118

EP - 124

JO - Journal of Ethnopharmacology

JF - Journal of Ethnopharmacology

SN - 0378-8741

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