Evaluation of the radioprotective effect of Aegle marmelos (L.) Correa in cultured human peripheral blood lymphocytes exposed to different doses of γ-radiation: A micronucleus study

Ganesh Chandra Jagetia, Ponemone Venkatesh, Manjeshwar Shrinath Baliga

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

The radioprotective effect of a hydroalcoholic extract of Aegle marmelos (AME) was evaluated in cultured human peripheral blood lymphocytes (HPBLs) by the micronucleus assay. The optimum protective dose of the extract was selected by treating HPBLs with 1.25, 2.5, 5, 6.25, 10, 20, 40, 60, 80 and 100 μg/ml AME before exposure to 3 Gy γ-radiation and then evaluating the micronucleus frequency in cytokinesis blocked HPBLs. Treatment of HPBLs with different doses of AME reduced the frequency of radiation-induced micronuclei significantly, with the greatest reduction in micronucleus induction being observed for 5 μg/ml AME. Therefore, this dose of AME was considered as the optimum dose for radioprotection and further studies were carried out treating the HPBLs with 5 μg/ml AME before exposure to different doses (0, 0.5, 1, 2, 3 and 4 Gy) of γ-radiation. The irradiation of HPBLs with different doses of γ-radiation caused a dose-dependent increase in the frequency of lymphocytes bearing one, two and multiple micronuclei, while treatment of HPBLs with 5 μg/ml AME significantly reduced the frequency of lymphocytes bearing one, two and multiple micronuclei when compared with the irradiated control. The dose-response relationship for both groups was linear. To understand the mechanism of action of AME separate experiments were conducted to evaluate the free radical scavenging of OH, O2-, DPPH, ABTS+ and NO in vitro. AME was found to inhibit free radicals in a dose-dependent manner up to a dose of 200 μg/ml for the majority of radicals and plateaued thereafter. Our study demonstrates that AME at 5 μg/ml protected HPBLs against radiation-induced DNA damage and genomic instability and its radioprotective activity may be by scavenging of radiation-induced free radicals and increased oxidant status.

Original languageEnglish
Pages (from-to)387-393
Number of pages7
JournalMutagenesis
Volume18
Issue number4
DOIs
Publication statusPublished - 01-07-2003
Externally publishedYes

Fingerprint

Aegle
Lymphocytes
Dosimetry
Blood
Radiation
Bearings (structural)
Free Radicals
Radiation Dosage
Scavenging
Micronucleus Tests
Cytokinesis
Genomic Instability
Oxidants
DNA Damage
Assays

All Science Journal Classification (ASJC) codes

  • Genetics
  • Toxicology
  • Genetics(clinical)
  • Health, Toxicology and Mutagenesis

Cite this

@article{5e2d594306ef40bda5d4ceeed0d123bf,
title = "Evaluation of the radioprotective effect of Aegle marmelos (L.) Correa in cultured human peripheral blood lymphocytes exposed to different doses of γ-radiation: A micronucleus study",
abstract = "The radioprotective effect of a hydroalcoholic extract of Aegle marmelos (AME) was evaluated in cultured human peripheral blood lymphocytes (HPBLs) by the micronucleus assay. The optimum protective dose of the extract was selected by treating HPBLs with 1.25, 2.5, 5, 6.25, 10, 20, 40, 60, 80 and 100 μg/ml AME before exposure to 3 Gy γ-radiation and then evaluating the micronucleus frequency in cytokinesis blocked HPBLs. Treatment of HPBLs with different doses of AME reduced the frequency of radiation-induced micronuclei significantly, with the greatest reduction in micronucleus induction being observed for 5 μg/ml AME. Therefore, this dose of AME was considered as the optimum dose for radioprotection and further studies were carried out treating the HPBLs with 5 μg/ml AME before exposure to different doses (0, 0.5, 1, 2, 3 and 4 Gy) of γ-radiation. The irradiation of HPBLs with different doses of γ-radiation caused a dose-dependent increase in the frequency of lymphocytes bearing one, two and multiple micronuclei, while treatment of HPBLs with 5 μg/ml AME significantly reduced the frequency of lymphocytes bearing one, two and multiple micronuclei when compared with the irradiated control. The dose-response relationship for both groups was linear. To understand the mechanism of action of AME separate experiments were conducted to evaluate the free radical scavenging of OH, O2-, DPPH, ABTS+ and NO in vitro. AME was found to inhibit free radicals in a dose-dependent manner up to a dose of 200 μg/ml for the majority of radicals and plateaued thereafter. Our study demonstrates that AME at 5 μg/ml protected HPBLs against radiation-induced DNA damage and genomic instability and its radioprotective activity may be by scavenging of radiation-induced free radicals and increased oxidant status.",
author = "Jagetia, {Ganesh Chandra} and Ponemone Venkatesh and Baliga, {Manjeshwar Shrinath}",
year = "2003",
month = "7",
day = "1",
doi = "10.1093/mutage/geg011",
language = "English",
volume = "18",
pages = "387--393",
journal = "Mutagenesis",
issn = "0267-8357",
publisher = "Oxford University Press",
number = "4",

}

Evaluation of the radioprotective effect of Aegle marmelos (L.) Correa in cultured human peripheral blood lymphocytes exposed to different doses of γ-radiation : A micronucleus study. / Jagetia, Ganesh Chandra; Venkatesh, Ponemone; Baliga, Manjeshwar Shrinath.

In: Mutagenesis, Vol. 18, No. 4, 01.07.2003, p. 387-393.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Evaluation of the radioprotective effect of Aegle marmelos (L.) Correa in cultured human peripheral blood lymphocytes exposed to different doses of γ-radiation

T2 - A micronucleus study

AU - Jagetia, Ganesh Chandra

AU - Venkatesh, Ponemone

AU - Baliga, Manjeshwar Shrinath

PY - 2003/7/1

Y1 - 2003/7/1

N2 - The radioprotective effect of a hydroalcoholic extract of Aegle marmelos (AME) was evaluated in cultured human peripheral blood lymphocytes (HPBLs) by the micronucleus assay. The optimum protective dose of the extract was selected by treating HPBLs with 1.25, 2.5, 5, 6.25, 10, 20, 40, 60, 80 and 100 μg/ml AME before exposure to 3 Gy γ-radiation and then evaluating the micronucleus frequency in cytokinesis blocked HPBLs. Treatment of HPBLs with different doses of AME reduced the frequency of radiation-induced micronuclei significantly, with the greatest reduction in micronucleus induction being observed for 5 μg/ml AME. Therefore, this dose of AME was considered as the optimum dose for radioprotection and further studies were carried out treating the HPBLs with 5 μg/ml AME before exposure to different doses (0, 0.5, 1, 2, 3 and 4 Gy) of γ-radiation. The irradiation of HPBLs with different doses of γ-radiation caused a dose-dependent increase in the frequency of lymphocytes bearing one, two and multiple micronuclei, while treatment of HPBLs with 5 μg/ml AME significantly reduced the frequency of lymphocytes bearing one, two and multiple micronuclei when compared with the irradiated control. The dose-response relationship for both groups was linear. To understand the mechanism of action of AME separate experiments were conducted to evaluate the free radical scavenging of OH, O2-, DPPH, ABTS+ and NO in vitro. AME was found to inhibit free radicals in a dose-dependent manner up to a dose of 200 μg/ml for the majority of radicals and plateaued thereafter. Our study demonstrates that AME at 5 μg/ml protected HPBLs against radiation-induced DNA damage and genomic instability and its radioprotective activity may be by scavenging of radiation-induced free radicals and increased oxidant status.

AB - The radioprotective effect of a hydroalcoholic extract of Aegle marmelos (AME) was evaluated in cultured human peripheral blood lymphocytes (HPBLs) by the micronucleus assay. The optimum protective dose of the extract was selected by treating HPBLs with 1.25, 2.5, 5, 6.25, 10, 20, 40, 60, 80 and 100 μg/ml AME before exposure to 3 Gy γ-radiation and then evaluating the micronucleus frequency in cytokinesis blocked HPBLs. Treatment of HPBLs with different doses of AME reduced the frequency of radiation-induced micronuclei significantly, with the greatest reduction in micronucleus induction being observed for 5 μg/ml AME. Therefore, this dose of AME was considered as the optimum dose for radioprotection and further studies were carried out treating the HPBLs with 5 μg/ml AME before exposure to different doses (0, 0.5, 1, 2, 3 and 4 Gy) of γ-radiation. The irradiation of HPBLs with different doses of γ-radiation caused a dose-dependent increase in the frequency of lymphocytes bearing one, two and multiple micronuclei, while treatment of HPBLs with 5 μg/ml AME significantly reduced the frequency of lymphocytes bearing one, two and multiple micronuclei when compared with the irradiated control. The dose-response relationship for both groups was linear. To understand the mechanism of action of AME separate experiments were conducted to evaluate the free radical scavenging of OH, O2-, DPPH, ABTS+ and NO in vitro. AME was found to inhibit free radicals in a dose-dependent manner up to a dose of 200 μg/ml for the majority of radicals and plateaued thereafter. Our study demonstrates that AME at 5 μg/ml protected HPBLs against radiation-induced DNA damage and genomic instability and its radioprotective activity may be by scavenging of radiation-induced free radicals and increased oxidant status.

UR - http://www.scopus.com/inward/record.url?scp=0038494640&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0038494640&partnerID=8YFLogxK

U2 - 10.1093/mutage/geg011

DO - 10.1093/mutage/geg011

M3 - Article

C2 - 12840113

AN - SCOPUS:0038494640

VL - 18

SP - 387

EP - 393

JO - Mutagenesis

JF - Mutagenesis

SN - 0267-8357

IS - 4

ER -