Evaluation of wound healing activity of ethanolic extract of Lantana camara in streptozotocin induced diabetic rats

Sivanageswararao Mekala, M. Kumar Naresh, Lipika Das, Nishmitha Shetty, Arul Amuthan, Venkatarao Vulli, Narasimharao Bhogireddy

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: Lantana camara (Verbanacea) is a commonly available medicinal plant throughout India. Wound healing property of the plant in various wound models has been studied. Thorough literature survey revealed that the wound healing property of Lantana camara in diabetic wound was not studied. This study was aimed to evaluate the wound healing property of Lantana camara in diabetic rats. Methods: Group-1 rats served as normal control in which excision wound was created in normal, non-diabetic rats and wound was topically applied with vehicle. To induce diabetes mellitus in group 2-5, a single injecting of streptozotocin (45 mg/kg, i.p.) prepared by dissolving in 0.9% ice cold citrate buffer was given. Excision wound was inflicted in the back of the rats. Group-2 was the diabetic control in which diabetic rats received vehicle ointment topically. Group 3, 4 and 5 were the test drug groups in which diabetic rats were topically applied ethanolic extract of Lantana camara in three doses 10%, 15% and 20% respectively. Wound healing parameters such as percentage of wound contraction rate and epithelialization period were observed. Data was analyzed using SPSS software by one way ANOVA and the statistical significance was fixed as p < 0.005. Results: There was a delay in wound healing in diabetic rats compared to non-diabetic rats. The extract showed dose dependent increase in wound contraction rate and hastened the epithelialization period. Extracts enhanced contraction rate only during later phase of wound healing process. High dose (20%) extract showed maximum healing effect. Conclusion: Topical application of ethanolic extract of Lantana camara showed dose dependent wound healing activity in diabetic rats.

Original languageEnglish
Pages (from-to)631-633
Number of pages3
JournalInternational Journal of Pharmacy and Pharmaceutical Sciences
Volume6
Issue number1
Publication statusPublished - 16-01-2014

Fingerprint

Lantana
Streptozocin
Wound Healing
Wounds and Injuries
Medicinal Plants
Ice
Ointments
Citric Acid
India
Diabetes Mellitus
Analysis of Variance
Buffers
Software

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science

Cite this

Mekala, Sivanageswararao ; Kumar Naresh, M. ; Das, Lipika ; Shetty, Nishmitha ; Amuthan, Arul ; Vulli, Venkatarao ; Bhogireddy, Narasimharao. / Evaluation of wound healing activity of ethanolic extract of Lantana camara in streptozotocin induced diabetic rats. In: International Journal of Pharmacy and Pharmaceutical Sciences. 2014 ; Vol. 6, No. 1. pp. 631-633.
@article{ca07c7462e8d4874b4572a055e1e8cf7,
title = "Evaluation of wound healing activity of ethanolic extract of Lantana camara in streptozotocin induced diabetic rats",
abstract = "Background: Lantana camara (Verbanacea) is a commonly available medicinal plant throughout India. Wound healing property of the plant in various wound models has been studied. Thorough literature survey revealed that the wound healing property of Lantana camara in diabetic wound was not studied. This study was aimed to evaluate the wound healing property of Lantana camara in diabetic rats. Methods: Group-1 rats served as normal control in which excision wound was created in normal, non-diabetic rats and wound was topically applied with vehicle. To induce diabetes mellitus in group 2-5, a single injecting of streptozotocin (45 mg/kg, i.p.) prepared by dissolving in 0.9{\%} ice cold citrate buffer was given. Excision wound was inflicted in the back of the rats. Group-2 was the diabetic control in which diabetic rats received vehicle ointment topically. Group 3, 4 and 5 were the test drug groups in which diabetic rats were topically applied ethanolic extract of Lantana camara in three doses 10{\%}, 15{\%} and 20{\%} respectively. Wound healing parameters such as percentage of wound contraction rate and epithelialization period were observed. Data was analyzed using SPSS software by one way ANOVA and the statistical significance was fixed as p < 0.005. Results: There was a delay in wound healing in diabetic rats compared to non-diabetic rats. The extract showed dose dependent increase in wound contraction rate and hastened the epithelialization period. Extracts enhanced contraction rate only during later phase of wound healing process. High dose (20{\%}) extract showed maximum healing effect. Conclusion: Topical application of ethanolic extract of Lantana camara showed dose dependent wound healing activity in diabetic rats.",
author = "Sivanageswararao Mekala and {Kumar Naresh}, M. and Lipika Das and Nishmitha Shetty and Arul Amuthan and Venkatarao Vulli and Narasimharao Bhogireddy",
year = "2014",
month = "1",
day = "16",
language = "English",
volume = "6",
pages = "631--633",
journal = "International Journal of Pharmacy and Pharmaceutical Sciences",
issn = "0975-1491",
publisher = "IJPPS",
number = "1",

}

Evaluation of wound healing activity of ethanolic extract of Lantana camara in streptozotocin induced diabetic rats. / Mekala, Sivanageswararao; Kumar Naresh, M.; Das, Lipika; Shetty, Nishmitha; Amuthan, Arul; Vulli, Venkatarao; Bhogireddy, Narasimharao.

In: International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 6, No. 1, 16.01.2014, p. 631-633.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Evaluation of wound healing activity of ethanolic extract of Lantana camara in streptozotocin induced diabetic rats

AU - Mekala, Sivanageswararao

AU - Kumar Naresh, M.

AU - Das, Lipika

AU - Shetty, Nishmitha

AU - Amuthan, Arul

AU - Vulli, Venkatarao

AU - Bhogireddy, Narasimharao

PY - 2014/1/16

Y1 - 2014/1/16

N2 - Background: Lantana camara (Verbanacea) is a commonly available medicinal plant throughout India. Wound healing property of the plant in various wound models has been studied. Thorough literature survey revealed that the wound healing property of Lantana camara in diabetic wound was not studied. This study was aimed to evaluate the wound healing property of Lantana camara in diabetic rats. Methods: Group-1 rats served as normal control in which excision wound was created in normal, non-diabetic rats and wound was topically applied with vehicle. To induce diabetes mellitus in group 2-5, a single injecting of streptozotocin (45 mg/kg, i.p.) prepared by dissolving in 0.9% ice cold citrate buffer was given. Excision wound was inflicted in the back of the rats. Group-2 was the diabetic control in which diabetic rats received vehicle ointment topically. Group 3, 4 and 5 were the test drug groups in which diabetic rats were topically applied ethanolic extract of Lantana camara in three doses 10%, 15% and 20% respectively. Wound healing parameters such as percentage of wound contraction rate and epithelialization period were observed. Data was analyzed using SPSS software by one way ANOVA and the statistical significance was fixed as p < 0.005. Results: There was a delay in wound healing in diabetic rats compared to non-diabetic rats. The extract showed dose dependent increase in wound contraction rate and hastened the epithelialization period. Extracts enhanced contraction rate only during later phase of wound healing process. High dose (20%) extract showed maximum healing effect. Conclusion: Topical application of ethanolic extract of Lantana camara showed dose dependent wound healing activity in diabetic rats.

AB - Background: Lantana camara (Verbanacea) is a commonly available medicinal plant throughout India. Wound healing property of the plant in various wound models has been studied. Thorough literature survey revealed that the wound healing property of Lantana camara in diabetic wound was not studied. This study was aimed to evaluate the wound healing property of Lantana camara in diabetic rats. Methods: Group-1 rats served as normal control in which excision wound was created in normal, non-diabetic rats and wound was topically applied with vehicle. To induce diabetes mellitus in group 2-5, a single injecting of streptozotocin (45 mg/kg, i.p.) prepared by dissolving in 0.9% ice cold citrate buffer was given. Excision wound was inflicted in the back of the rats. Group-2 was the diabetic control in which diabetic rats received vehicle ointment topically. Group 3, 4 and 5 were the test drug groups in which diabetic rats were topically applied ethanolic extract of Lantana camara in three doses 10%, 15% and 20% respectively. Wound healing parameters such as percentage of wound contraction rate and epithelialization period were observed. Data was analyzed using SPSS software by one way ANOVA and the statistical significance was fixed as p < 0.005. Results: There was a delay in wound healing in diabetic rats compared to non-diabetic rats. The extract showed dose dependent increase in wound contraction rate and hastened the epithelialization period. Extracts enhanced contraction rate only during later phase of wound healing process. High dose (20%) extract showed maximum healing effect. Conclusion: Topical application of ethanolic extract of Lantana camara showed dose dependent wound healing activity in diabetic rats.

UR - http://www.scopus.com/inward/record.url?scp=84891914481&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84891914481&partnerID=8YFLogxK

M3 - Article

VL - 6

SP - 631

EP - 633

JO - International Journal of Pharmacy and Pharmaceutical Sciences

JF - International Journal of Pharmacy and Pharmaceutical Sciences

SN - 0975-1491

IS - 1

ER -