Evidence that estrogen directly and indirectly modulates C1 adrenergic bulbospinal neurons in the rostral ventrolateral medulla

Gang Wang, Carrie T. Drake, Mariya Rozenblit, Ping Zhou, Stephen E. Alves, Scott P. Herrick, Shinji Hayashi, Sudha Warrier, Costantino Iadecola, Teresa A. Milner

Research output: Contribution to journalArticle

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Abstract

Blood pressure in women increases after menopause, and sympathetic tone in female rats decreases with estrogen injections in the rostral ventrolateral medulla (RVLM) region that contains bulbospinal C1 adrenergic neurons and is involved in blood pressure control. We investigated the anatomical and physiological basis for estrogen effects in the RVLM. Neurons with α- or β-subtypes of estrogen receptor (ER) immunoreactivity (-ir) overlapped in distribution with tyrosine hydroxylase (TH)-containing C1 neurons. Immunoelectron microscopy revealed that ERα- and ERβ-ir had distinct cellular and subcellular distributions. ERα-ir was most commonly in TH-lacking profiles, many of which were axons and peptide-containing afferents that contacted TH-containing dendrites. ERα-ir was also in some TH-containing dendrites. ERβ-ir was most frequently in TH-containing somata and dendrites, particularly on endoplasmic reticula, mitochondria, and plasma membranes. In whole-cell patch clamp recordings from isolated bulbospinal RVLM neurons, 17β-estradiol dose-dependently reduced voltage-gated Ca++ currents, especially the long-lasting (L-type) component. This inhibition was reversed by washing or prevented by adding the non-subtype-selective ER antagonist ICI182780. An ERβ-selective agonist, but not an ERα-selective agonist, reproduced the Ca++ current inhibition. The data indicate that estrogens can modulate the function of RVLM C1 bulbospinal neurons either directly, through extranuclear ERβ, or indirectly through extranuclear ERα in selected afferents. Moreover, Ca++ current inhibition may underlie the decrease in sympathetic tone evoked by local 17β-estradiol application. These findings provide a structural and functional basis for the effects of estrogens on blood pressure control and suggest a mechanism for the modulation of cardiovascular function by estrogen in women.

Original languageEnglish
Pages (from-to)163-178
Number of pages16
JournalBrain Research
Volume1094
Issue number1
DOIs
Publication statusPublished - 13-06-2006
Externally publishedYes

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Adrenergic Neurons
Estrogen Receptors
Estrogens
Tyrosine 3-Monooxygenase
Dendrites
Neurons
Blood Pressure
Estradiol
Immunoelectron Microscopy
Carisoprodol
Menopause
Endoplasmic Reticulum
Axons
Mitochondria
Cell Membrane
Peptides
Injections

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Wang, G., Drake, C. T., Rozenblit, M., Zhou, P., Alves, S. E., Herrick, S. P., ... Milner, T. A. (2006). Evidence that estrogen directly and indirectly modulates C1 adrenergic bulbospinal neurons in the rostral ventrolateral medulla. Brain Research, 1094(1), 163-178. https://doi.org/10.1016/j.brainres.2006.03.089
Wang, Gang ; Drake, Carrie T. ; Rozenblit, Mariya ; Zhou, Ping ; Alves, Stephen E. ; Herrick, Scott P. ; Hayashi, Shinji ; Warrier, Sudha ; Iadecola, Costantino ; Milner, Teresa A. / Evidence that estrogen directly and indirectly modulates C1 adrenergic bulbospinal neurons in the rostral ventrolateral medulla. In: Brain Research. 2006 ; Vol. 1094, No. 1. pp. 163-178.
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Wang, G, Drake, CT, Rozenblit, M, Zhou, P, Alves, SE, Herrick, SP, Hayashi, S, Warrier, S, Iadecola, C & Milner, TA 2006, 'Evidence that estrogen directly and indirectly modulates C1 adrenergic bulbospinal neurons in the rostral ventrolateral medulla', Brain Research, vol. 1094, no. 1, pp. 163-178. https://doi.org/10.1016/j.brainres.2006.03.089

Evidence that estrogen directly and indirectly modulates C1 adrenergic bulbospinal neurons in the rostral ventrolateral medulla. / Wang, Gang; Drake, Carrie T.; Rozenblit, Mariya; Zhou, Ping; Alves, Stephen E.; Herrick, Scott P.; Hayashi, Shinji; Warrier, Sudha; Iadecola, Costantino; Milner, Teresa A.

In: Brain Research, Vol. 1094, No. 1, 13.06.2006, p. 163-178.

Research output: Contribution to journalArticle

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T1 - Evidence that estrogen directly and indirectly modulates C1 adrenergic bulbospinal neurons in the rostral ventrolateral medulla

AU - Wang, Gang

AU - Drake, Carrie T.

AU - Rozenblit, Mariya

AU - Zhou, Ping

AU - Alves, Stephen E.

AU - Herrick, Scott P.

AU - Hayashi, Shinji

AU - Warrier, Sudha

AU - Iadecola, Costantino

AU - Milner, Teresa A.

PY - 2006/6/13

Y1 - 2006/6/13

N2 - Blood pressure in women increases after menopause, and sympathetic tone in female rats decreases with estrogen injections in the rostral ventrolateral medulla (RVLM) region that contains bulbospinal C1 adrenergic neurons and is involved in blood pressure control. We investigated the anatomical and physiological basis for estrogen effects in the RVLM. Neurons with α- or β-subtypes of estrogen receptor (ER) immunoreactivity (-ir) overlapped in distribution with tyrosine hydroxylase (TH)-containing C1 neurons. Immunoelectron microscopy revealed that ERα- and ERβ-ir had distinct cellular and subcellular distributions. ERα-ir was most commonly in TH-lacking profiles, many of which were axons and peptide-containing afferents that contacted TH-containing dendrites. ERα-ir was also in some TH-containing dendrites. ERβ-ir was most frequently in TH-containing somata and dendrites, particularly on endoplasmic reticula, mitochondria, and plasma membranes. In whole-cell patch clamp recordings from isolated bulbospinal RVLM neurons, 17β-estradiol dose-dependently reduced voltage-gated Ca++ currents, especially the long-lasting (L-type) component. This inhibition was reversed by washing or prevented by adding the non-subtype-selective ER antagonist ICI182780. An ERβ-selective agonist, but not an ERα-selective agonist, reproduced the Ca++ current inhibition. The data indicate that estrogens can modulate the function of RVLM C1 bulbospinal neurons either directly, through extranuclear ERβ, or indirectly through extranuclear ERα in selected afferents. Moreover, Ca++ current inhibition may underlie the decrease in sympathetic tone evoked by local 17β-estradiol application. These findings provide a structural and functional basis for the effects of estrogens on blood pressure control and suggest a mechanism for the modulation of cardiovascular function by estrogen in women.

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