Expansion and characterization of bone marrow derived human mesenchymal stromal cells in serum free conditions

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Abstract

Bone marrow-derived mesenchymal stromal cells (BM-MSCs) are gaining increasing importance in the feld of regenerative medicine. Although therapeutic value of MSCs is now being established through many clinical trials, issues have been raised regarding their expansion as per regulatory guidelines. Fetal bovine serum usage in cell therapy poses difculties due to its less-defned, highly variable
composition and safety issues. Hence, there is a need for transition from serum-based to serum-free media (SFM). Since SFM are cell type-specifc, a precise analysis of the properties of MSCs cultured in SFM is required to determine the most suitable one. Six diferent commercially available low serum/SFM with two diferent seeding densities were evaluated to explore their ability to support the growth and expansion of BM-MSCs and assess the characteristics of BM-MSCs cultured in these media. Except for one of the SFM, all other media tested supported the growth of BM-MSCs at a low seeding density. No signifcant diferences were observed in the expression of MSC specifc markers among the various media tested. In contrary, the population doubling time, cell yield, potency, colony-forming ability, diferentiation potential, and immunosuppressive properties of MSCs varied with one another. We show that SFM tested supports the growth and expansion of BM-MSCs even at low seeding density and may serve as possible replacement for animal-derived serum.
Original languageEnglish
Article numberhttps://doi.org/10.1038/s41598-021-83088-1
JournalScientific Reports
Volume11
Issue number3403
Publication statusPublished - 09-02-2021

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