Experimental therapeutic intervention with alpha tocopherol in ethanol induced testicular injuries in rats

M. Maneesh, Sanjiba Dutta, Amit Chakrabarti, D. M. Vasudevan

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Infertility is well-established harmful effect in chronic alcoholism and so far, there is no effective treatment for this condition. The study was conducted to determine the effects of alpha tocopherol on ethanol induced testicular injuries in male albino rats of Wistar strain. Five groups (n=6) of animals were used. Group I served as control. Group II received daily 1.6g ethanol/kg body weight/day for 4 weeks orally. Group III received 1.6g ethanol + 80mg alpha tocopherol/kg body weight/day for four weeks orally. Group IV received 1.6g ethanol /kg body weight for/day 4 weeks and followed by 80mg alpha tocopherol/kg body weight/day for four weeks orally. Group V received 1.6g ethanol/kg body weight/day orally for 4 weeks, followed by 4 weeks abstinence. Twentyfour hours after the last treatment the rats were sacrificed using anesthetic ether. Testes were removed and used for the estimation of extent of lipid peroxidation and tissue levels of antioxidants and steroidogenic enzymes. Alpha tocopherol treatment increased the activities of testicular Δ5, 3β-HSD and 17β-HSD. Moreover, the treatment was also associated with significant decrease in testicular oxidative stress. Ethanol-induced oxidative stress and decreased steroidogenesis can be reversed by treatment with alpha tocopherol.

Original languageEnglish
Pages (from-to)138-142
Number of pages5
JournalIndian Journal of Clinical Biochemistry
Volume22
Issue number1
DOIs
Publication statusPublished - 19-03-2007
Externally publishedYes

Fingerprint

alpha-Tocopherol
Rats
Ethanol
Body Weight
Wounds and Injuries
Oxidative stress
Oxidative Stress
Therapeutics
Ether
Infertility
Lipid Peroxidation
Alcoholism
Anesthetics
Testis
Wistar Rats
Animals
Antioxidants
Tissue
Lipids
Enzymes

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry

Cite this

Maneesh, M. ; Dutta, Sanjiba ; Chakrabarti, Amit ; Vasudevan, D. M. / Experimental therapeutic intervention with alpha tocopherol in ethanol induced testicular injuries in rats. In: Indian Journal of Clinical Biochemistry. 2007 ; Vol. 22, No. 1. pp. 138-142.
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Experimental therapeutic intervention with alpha tocopherol in ethanol induced testicular injuries in rats. / Maneesh, M.; Dutta, Sanjiba; Chakrabarti, Amit; Vasudevan, D. M.

In: Indian Journal of Clinical Biochemistry, Vol. 22, No. 1, 19.03.2007, p. 138-142.

Research output: Contribution to journalArticle

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