Abstract
Prepubertal Swiss albino mice of both sex were administered with first-line anti-tuberculosis drugs (ATDs) viz; rifampicin, isoniazid, pyrazinamide, streptomycin and ethambutol intraperitoneally, for 4 weeks. Two weeks after the completion of treatment, male mice were sacrificed to collect caudal spermatozoa and female mice were superovulated with pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG) to collect metaphase II (MII) oocytes from oviduct. Administration of ATDs not only decreased the count, motility and, nuclear maturity and also, increased the head abnormalities, mitochondrial damage and DNA damage in epididymal spermatozoa. Reduction in number of ovulated oocytes, increased degeneration rate and altered distribution pattern of cytoplasmic organelles was observed in oocytes of female mice. Presence of ATDs in in vitro maturation (IVM) medium increased abnormalities in meiotic resulted in abnormal spindle organization (except ethambutol) without affecting nuclear maturation. In conclusion, the result of this study indicates that ATDs have considerable adverse effects on the functional competence of male and female gametes, however, with varied degree of toxicity.
| Original language | English |
|---|---|
| Article number | 103292 |
| Journal | Environmental Toxicology and Pharmacology |
| Volume | 73 |
| DOIs | |
| Publication status | Published - 01-2020 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Toxicology
- Pharmacology
- Health, Toxicology and Mutagenesis
Cite this
}
Exposure to first line anti-tuberculosis drugs in prepubertal age reduces the quality and functional competence of spermatozoa and oocytes in Swiss albino mice. / Rao, Arpitha; Nayak, Guruprasad; Kumari, Sandhya; Kalthur, Sneha Guruprasad; Mutalik, Sadhana P.; Mutalik, Srinivas; Adiga, Satish Kumar; Kalthur, Guruprasad.
In: Environmental Toxicology and Pharmacology, Vol. 73, 103292, 01.2020.Research output: Contribution to journal › Article
TY - JOUR
T1 - Exposure to first line anti-tuberculosis drugs in prepubertal age reduces the quality and functional competence of spermatozoa and oocytes in Swiss albino mice
AU - Rao, Arpitha
AU - Nayak, Guruprasad
AU - Kumari, Sandhya
AU - Kalthur, Sneha Guruprasad
AU - Mutalik, Sadhana P.
AU - Mutalik, Srinivas
AU - Adiga, Satish Kumar
AU - Kalthur, Guruprasad
PY - 2020/1
Y1 - 2020/1
N2 - Prepubertal Swiss albino mice of both sex were administered with first-line anti-tuberculosis drugs (ATDs) viz; rifampicin, isoniazid, pyrazinamide, streptomycin and ethambutol intraperitoneally, for 4 weeks. Two weeks after the completion of treatment, male mice were sacrificed to collect caudal spermatozoa and female mice were superovulated with pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG) to collect metaphase II (MII) oocytes from oviduct. Administration of ATDs not only decreased the count, motility and, nuclear maturity and also, increased the head abnormalities, mitochondrial damage and DNA damage in epididymal spermatozoa. Reduction in number of ovulated oocytes, increased degeneration rate and altered distribution pattern of cytoplasmic organelles was observed in oocytes of female mice. Presence of ATDs in in vitro maturation (IVM) medium increased abnormalities in meiotic resulted in abnormal spindle organization (except ethambutol) without affecting nuclear maturation. In conclusion, the result of this study indicates that ATDs have considerable adverse effects on the functional competence of male and female gametes, however, with varied degree of toxicity.
AB - Prepubertal Swiss albino mice of both sex were administered with first-line anti-tuberculosis drugs (ATDs) viz; rifampicin, isoniazid, pyrazinamide, streptomycin and ethambutol intraperitoneally, for 4 weeks. Two weeks after the completion of treatment, male mice were sacrificed to collect caudal spermatozoa and female mice were superovulated with pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG) to collect metaphase II (MII) oocytes from oviduct. Administration of ATDs not only decreased the count, motility and, nuclear maturity and also, increased the head abnormalities, mitochondrial damage and DNA damage in epididymal spermatozoa. Reduction in number of ovulated oocytes, increased degeneration rate and altered distribution pattern of cytoplasmic organelles was observed in oocytes of female mice. Presence of ATDs in in vitro maturation (IVM) medium increased abnormalities in meiotic resulted in abnormal spindle organization (except ethambutol) without affecting nuclear maturation. In conclusion, the result of this study indicates that ATDs have considerable adverse effects on the functional competence of male and female gametes, however, with varied degree of toxicity.
UR - http://www.scopus.com/inward/record.url?scp=85075211348&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85075211348&partnerID=8YFLogxK
U2 - 10.1016/j.etap.2019.103292
DO - 10.1016/j.etap.2019.103292
M3 - Article
AN - SCOPUS:85075211348
VL - 73
JO - Environmental Toxicology and Pharmacology
JF - Environmental Toxicology and Pharmacology
SN - 1382-6689
M1 - 103292
ER -