Expression dynamics of the poly-γ-glutamic acid biosynthesis genes of Bacillus subtilis in response to glucose and glutamic acid-a pilot study

Deepika Pandey Tiwari, Poonam Mishra Chatterjee, Harish Rotti, Bipin Chand, Ritu Raval, Ashok Kumar Dubey

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Poly-γ-glutamic acid (PGA) is biosynthesized by various Bacillus species through PGA synthetase, encoded by the PGA operon comprised of the ywsC and ywtABC genes. Due to the minimal available knowledge, understanding the expression pattern of PGA operon genes is pivotal. In this study, the effect of glucose and glutamic acid on the global gene expression profile of Bacillus subtilis Natto3 was investigated using high throughput microarray, with an emphasis on the PGA operon and genes influencing PGA production. Two treatment groups (set1-in the presence of glutamic acid and set2-in the presence of glutamic acid + glucose) were analyzed against the control (in the presence of glucose). In the microarray, both the groups showed a trend of up-regulation for ywsC and ywtA genes (log2 fold change of 0.55, P = 0.0194, 0.92, P = 0.0069 in set1 and 0.78, P = 0.0023, 0.59, P = 0.0172 in set2, respectively) and down-regulation of ywtB and ywtC genes (log2 fold change of -1.83, P = 0.0001, -1.42, P = 0.0017 in set1 and -1.52, P = 0.0012, -0.55, P = 0.1112 in set2, respectively), supporting the indispensability of the ywsC and ywtA genes in PGA production. Interestingly, the ywtB and ywtC genes, belonging to the same operon, were down-regulated in both the conditions (set1 and set2). To the best of our knowledge, this expression pattern of PGA operon genes is a unique observation.

Original languageEnglish
JournalFEMS Microbiology Letters
Volume365
Issue number22
DOIs
Publication statusPublished - 01-11-2018

Fingerprint

Bacillus subtilis
Glutamic Acid
Glucose
Operon
Genes
Ligases
Transcriptome
Bacillus
Up-Regulation
Down-Regulation
Observation

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Genetics

Cite this

Tiwari, Deepika Pandey ; Chatterjee, Poonam Mishra ; Rotti, Harish ; Chand, Bipin ; Raval, Ritu ; Dubey, Ashok Kumar. / Expression dynamics of the poly-γ-glutamic acid biosynthesis genes of Bacillus subtilis in response to glucose and glutamic acid-a pilot study. In: FEMS Microbiology Letters. 2018 ; Vol. 365, No. 22.
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abstract = "Poly-γ-glutamic acid (PGA) is biosynthesized by various Bacillus species through PGA synthetase, encoded by the PGA operon comprised of the ywsC and ywtABC genes. Due to the minimal available knowledge, understanding the expression pattern of PGA operon genes is pivotal. In this study, the effect of glucose and glutamic acid on the global gene expression profile of Bacillus subtilis Natto3 was investigated using high throughput microarray, with an emphasis on the PGA operon and genes influencing PGA production. Two treatment groups (set1-in the presence of glutamic acid and set2-in the presence of glutamic acid + glucose) were analyzed against the control (in the presence of glucose). In the microarray, both the groups showed a trend of up-regulation for ywsC and ywtA genes (log2 fold change of 0.55, P = 0.0194, 0.92, P = 0.0069 in set1 and 0.78, P = 0.0023, 0.59, P = 0.0172 in set2, respectively) and down-regulation of ywtB and ywtC genes (log2 fold change of -1.83, P = 0.0001, -1.42, P = 0.0017 in set1 and -1.52, P = 0.0012, -0.55, P = 0.1112 in set2, respectively), supporting the indispensability of the ywsC and ywtA genes in PGA production. Interestingly, the ywtB and ywtC genes, belonging to the same operon, were down-regulated in both the conditions (set1 and set2). To the best of our knowledge, this expression pattern of PGA operon genes is a unique observation.",
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Expression dynamics of the poly-γ-glutamic acid biosynthesis genes of Bacillus subtilis in response to glucose and glutamic acid-a pilot study. / Tiwari, Deepika Pandey; Chatterjee, Poonam Mishra; Rotti, Harish; Chand, Bipin; Raval, Ritu; Dubey, Ashok Kumar.

In: FEMS Microbiology Letters, Vol. 365, No. 22, 01.11.2018.

Research output: Contribution to journalArticle

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