Expression of biologically active Hemagglutinin-neuraminidase protein of Peste des petits ruminants virus in transgenic pigeonpea [Cajanus cajan (L) Millsp.]

V. Prasad, V. V. Satyavathi, Sanjaya, K. M. Valli, Abha Khandelwal, M. S. Shaila, G. Lakshmi Sita

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Hemagglutinin-neuraminidase (HN) gene of Peste des petits ruminants virus (PPRV) has been expressed in pigeonpea for the development of an edible vaccine for Peste des Petits ruminant (PPR). PPRV, causes PPR disease in sheep and goats with high mortality rate. The two surface glycoproteins of PPRV Hemagglutinin-neuraminidase and fusion protein (F) confer protective immunity. We report the successful generation of transgenic pigeonpea (Cajanus cajan (L.) Millsp.) plants and expression of HN protein having biological activity. A 2kb fragment containing the coding region of the HN gene from an Indian isolate was cloned into the binary vector pBI121 and mobilized into Agrobacterium tumefaciens strain GV3 101. Cotyledonary nodes from germinated seeds of pigeonpea were used for transformation. The presence of transgenes, NPTII and HN in the plants was confirmed by PCR. The expression of HN protein in the transgenic lines was further confirmed by Western blot analysis using polyclonal monospecific antibody to HN and more importantly plant-derived HN protein was shown to be biologically active as demonstrated by neuraminidase activity. Transgenic plants were fertile and PCR of T1 plants confirmed the inheritance of the transgene.

Original languageEnglish
Pages (from-to)199-205
Number of pages7
JournalPlant Science
Volume166
Issue number1
DOIs
Publication statusPublished - 01-01-2004
Externally publishedYes

Fingerprint

Peste-des-petits-ruminants virus
Cajanus
sialidase
Cajanus cajan
Hemagglutinins
Neuraminidase
hemagglutinins
genetically modified organisms
Proteins
proteins
Peste-des-Petits-Ruminants
Plant Lectins
peste des petits ruminants
Transgenes
transgenes
Edible Vaccines
Goat Diseases
edible vaccines
Sheep Diseases
Far-Western Blotting

All Science Journal Classification (ASJC) codes

  • Genetics
  • Agronomy and Crop Science
  • Plant Science

Cite this

Prasad, V. ; Satyavathi, V. V. ; Sanjaya ; Valli, K. M. ; Khandelwal, Abha ; Shaila, M. S. ; Sita, G. Lakshmi. / Expression of biologically active Hemagglutinin-neuraminidase protein of Peste des petits ruminants virus in transgenic pigeonpea [Cajanus cajan (L) Millsp.]. In: Plant Science. 2004 ; Vol. 166, No. 1. pp. 199-205.
@article{7ba0f096fd2c4eceabb680b89467c627,
title = "Expression of biologically active Hemagglutinin-neuraminidase protein of Peste des petits ruminants virus in transgenic pigeonpea [Cajanus cajan (L) Millsp.]",
abstract = "Hemagglutinin-neuraminidase (HN) gene of Peste des petits ruminants virus (PPRV) has been expressed in pigeonpea for the development of an edible vaccine for Peste des Petits ruminant (PPR). PPRV, causes PPR disease in sheep and goats with high mortality rate. The two surface glycoproteins of PPRV Hemagglutinin-neuraminidase and fusion protein (F) confer protective immunity. We report the successful generation of transgenic pigeonpea (Cajanus cajan (L.) Millsp.) plants and expression of HN protein having biological activity. A 2kb fragment containing the coding region of the HN gene from an Indian isolate was cloned into the binary vector pBI121 and mobilized into Agrobacterium tumefaciens strain GV3 101. Cotyledonary nodes from germinated seeds of pigeonpea were used for transformation. The presence of transgenes, NPTII and HN in the plants was confirmed by PCR. The expression of HN protein in the transgenic lines was further confirmed by Western blot analysis using polyclonal monospecific antibody to HN and more importantly plant-derived HN protein was shown to be biologically active as demonstrated by neuraminidase activity. Transgenic plants were fertile and PCR of T1 plants confirmed the inheritance of the transgene.",
author = "V. Prasad and Satyavathi, {V. V.} and Sanjaya and Valli, {K. M.} and Abha Khandelwal and Shaila, {M. S.} and Sita, {G. Lakshmi}",
year = "2004",
month = "1",
day = "1",
doi = "10.1016/j.plantsci.2003.08.020",
language = "English",
volume = "166",
pages = "199--205",
journal = "Plant Science",
issn = "0168-9452",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

Expression of biologically active Hemagglutinin-neuraminidase protein of Peste des petits ruminants virus in transgenic pigeonpea [Cajanus cajan (L) Millsp.]. / Prasad, V.; Satyavathi, V. V.; Sanjaya; Valli, K. M.; Khandelwal, Abha; Shaila, M. S.; Sita, G. Lakshmi.

In: Plant Science, Vol. 166, No. 1, 01.01.2004, p. 199-205.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Expression of biologically active Hemagglutinin-neuraminidase protein of Peste des petits ruminants virus in transgenic pigeonpea [Cajanus cajan (L) Millsp.]

AU - Prasad, V.

AU - Satyavathi, V. V.

AU - Sanjaya,

AU - Valli, K. M.

AU - Khandelwal, Abha

AU - Shaila, M. S.

AU - Sita, G. Lakshmi

PY - 2004/1/1

Y1 - 2004/1/1

N2 - Hemagglutinin-neuraminidase (HN) gene of Peste des petits ruminants virus (PPRV) has been expressed in pigeonpea for the development of an edible vaccine for Peste des Petits ruminant (PPR). PPRV, causes PPR disease in sheep and goats with high mortality rate. The two surface glycoproteins of PPRV Hemagglutinin-neuraminidase and fusion protein (F) confer protective immunity. We report the successful generation of transgenic pigeonpea (Cajanus cajan (L.) Millsp.) plants and expression of HN protein having biological activity. A 2kb fragment containing the coding region of the HN gene from an Indian isolate was cloned into the binary vector pBI121 and mobilized into Agrobacterium tumefaciens strain GV3 101. Cotyledonary nodes from germinated seeds of pigeonpea were used for transformation. The presence of transgenes, NPTII and HN in the plants was confirmed by PCR. The expression of HN protein in the transgenic lines was further confirmed by Western blot analysis using polyclonal monospecific antibody to HN and more importantly plant-derived HN protein was shown to be biologically active as demonstrated by neuraminidase activity. Transgenic plants were fertile and PCR of T1 plants confirmed the inheritance of the transgene.

AB - Hemagglutinin-neuraminidase (HN) gene of Peste des petits ruminants virus (PPRV) has been expressed in pigeonpea for the development of an edible vaccine for Peste des Petits ruminant (PPR). PPRV, causes PPR disease in sheep and goats with high mortality rate. The two surface glycoproteins of PPRV Hemagglutinin-neuraminidase and fusion protein (F) confer protective immunity. We report the successful generation of transgenic pigeonpea (Cajanus cajan (L.) Millsp.) plants and expression of HN protein having biological activity. A 2kb fragment containing the coding region of the HN gene from an Indian isolate was cloned into the binary vector pBI121 and mobilized into Agrobacterium tumefaciens strain GV3 101. Cotyledonary nodes from germinated seeds of pigeonpea were used for transformation. The presence of transgenes, NPTII and HN in the plants was confirmed by PCR. The expression of HN protein in the transgenic lines was further confirmed by Western blot analysis using polyclonal monospecific antibody to HN and more importantly plant-derived HN protein was shown to be biologically active as demonstrated by neuraminidase activity. Transgenic plants were fertile and PCR of T1 plants confirmed the inheritance of the transgene.

UR - http://www.scopus.com/inward/record.url?scp=1642444883&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1642444883&partnerID=8YFLogxK

U2 - 10.1016/j.plantsci.2003.08.020

DO - 10.1016/j.plantsci.2003.08.020

M3 - Article

AN - SCOPUS:1642444883

VL - 166

SP - 199

EP - 205

JO - Plant Science

JF - Plant Science

SN - 0168-9452

IS - 1

ER -