Expression of epithelial cell adhesion molecule (EpCAM) in oral squamous cell carcinoma

Subhalakshmi Sen, Sunitha Carnelio

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Aims: To study the expression of epithelial cell adhesion molecule (EpCAM) in oral squamous cell carcinoma (OSCC) by immunohistochemistry and investigate its association with various clinicopathological parameters, including patient survival. Methods and results: This cross-sectional study was carried out on 60 histologically confirmed cases of OSCC. EpCAM expression was evaluated by the indirect streptavidin-biotin method and was then assessed semiquantitatively in terms of staining proportion, staining intensity and total staining. The immunoprofile of EpCAM was correlated with clinicopathological parameters such as gender, habit, tumour size, site, regional lymph node and distant metastasis, clinical stage, histological grade, local tumour recurrence and patient survival. EpCAM expression was found to be absent in normal oral mucosa. However, positive staining was seen in 51 of 60 OSCC cases and EpCAM overexpression in 11 specimens. Overexpression of EpCAM was associated significantly with tumour size, histological grade, local recurrence of tumour and patient survival. Conclusions: EpCAM serves as a potential biomarker of prognostic significance that could be used to identify patients at high risk and to predict patient survival. Moreover, EpCAM antigen represents an attractive target for immunotherapeutic approaches in a subgroup of patients with EpCAM-positive OSCC.

Original languageEnglish
Pages (from-to)897-904
Number of pages8
JournalHistopathology
Volume68
Issue number6
DOIs
Publication statusPublished - 01-05-2016

Fingerprint

Squamous Cell Carcinoma
Staining and Labeling
Survival
Neoplasms
Epithelial Cell Adhesion Molecule
Recurrence
Streptavidin
Mouth Mucosa
Biotin
Habits
Cross-Sectional Studies
Biomarkers
Lymph Nodes
Immunohistochemistry
Neoplasm Metastasis
Antigens

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Histology

Cite this

@article{8aafe3e4817a4ca989ca86e747dbe27f,
title = "Expression of epithelial cell adhesion molecule (EpCAM) in oral squamous cell carcinoma",
abstract = "Aims: To study the expression of epithelial cell adhesion molecule (EpCAM) in oral squamous cell carcinoma (OSCC) by immunohistochemistry and investigate its association with various clinicopathological parameters, including patient survival. Methods and results: This cross-sectional study was carried out on 60 histologically confirmed cases of OSCC. EpCAM expression was evaluated by the indirect streptavidin-biotin method and was then assessed semiquantitatively in terms of staining proportion, staining intensity and total staining. The immunoprofile of EpCAM was correlated with clinicopathological parameters such as gender, habit, tumour size, site, regional lymph node and distant metastasis, clinical stage, histological grade, local tumour recurrence and patient survival. EpCAM expression was found to be absent in normal oral mucosa. However, positive staining was seen in 51 of 60 OSCC cases and EpCAM overexpression in 11 specimens. Overexpression of EpCAM was associated significantly with tumour size, histological grade, local recurrence of tumour and patient survival. Conclusions: EpCAM serves as a potential biomarker of prognostic significance that could be used to identify patients at high risk and to predict patient survival. Moreover, EpCAM antigen represents an attractive target for immunotherapeutic approaches in a subgroup of patients with EpCAM-positive OSCC.",
author = "Subhalakshmi Sen and Sunitha Carnelio",
year = "2016",
month = "5",
day = "1",
doi = "10.1111/his.12870",
language = "English",
volume = "68",
pages = "897--904",
journal = "Histopathology",
issn = "0309-0167",
publisher = "Wiley-Blackwell",
number = "6",

}

Expression of epithelial cell adhesion molecule (EpCAM) in oral squamous cell carcinoma. / Sen, Subhalakshmi; Carnelio, Sunitha.

In: Histopathology, Vol. 68, No. 6, 01.05.2016, p. 897-904.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Expression of epithelial cell adhesion molecule (EpCAM) in oral squamous cell carcinoma

AU - Sen, Subhalakshmi

AU - Carnelio, Sunitha

PY - 2016/5/1

Y1 - 2016/5/1

N2 - Aims: To study the expression of epithelial cell adhesion molecule (EpCAM) in oral squamous cell carcinoma (OSCC) by immunohistochemistry and investigate its association with various clinicopathological parameters, including patient survival. Methods and results: This cross-sectional study was carried out on 60 histologically confirmed cases of OSCC. EpCAM expression was evaluated by the indirect streptavidin-biotin method and was then assessed semiquantitatively in terms of staining proportion, staining intensity and total staining. The immunoprofile of EpCAM was correlated with clinicopathological parameters such as gender, habit, tumour size, site, regional lymph node and distant metastasis, clinical stage, histological grade, local tumour recurrence and patient survival. EpCAM expression was found to be absent in normal oral mucosa. However, positive staining was seen in 51 of 60 OSCC cases and EpCAM overexpression in 11 specimens. Overexpression of EpCAM was associated significantly with tumour size, histological grade, local recurrence of tumour and patient survival. Conclusions: EpCAM serves as a potential biomarker of prognostic significance that could be used to identify patients at high risk and to predict patient survival. Moreover, EpCAM antigen represents an attractive target for immunotherapeutic approaches in a subgroup of patients with EpCAM-positive OSCC.

AB - Aims: To study the expression of epithelial cell adhesion molecule (EpCAM) in oral squamous cell carcinoma (OSCC) by immunohistochemistry and investigate its association with various clinicopathological parameters, including patient survival. Methods and results: This cross-sectional study was carried out on 60 histologically confirmed cases of OSCC. EpCAM expression was evaluated by the indirect streptavidin-biotin method and was then assessed semiquantitatively in terms of staining proportion, staining intensity and total staining. The immunoprofile of EpCAM was correlated with clinicopathological parameters such as gender, habit, tumour size, site, regional lymph node and distant metastasis, clinical stage, histological grade, local tumour recurrence and patient survival. EpCAM expression was found to be absent in normal oral mucosa. However, positive staining was seen in 51 of 60 OSCC cases and EpCAM overexpression in 11 specimens. Overexpression of EpCAM was associated significantly with tumour size, histological grade, local recurrence of tumour and patient survival. Conclusions: EpCAM serves as a potential biomarker of prognostic significance that could be used to identify patients at high risk and to predict patient survival. Moreover, EpCAM antigen represents an attractive target for immunotherapeutic approaches in a subgroup of patients with EpCAM-positive OSCC.

UR - http://www.scopus.com/inward/record.url?scp=84948152604&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84948152604&partnerID=8YFLogxK

U2 - 10.1111/his.12870

DO - 10.1111/his.12870

M3 - Article

VL - 68

SP - 897

EP - 904

JO - Histopathology

JF - Histopathology

SN - 0309-0167

IS - 6

ER -