Expression of hemagglutinin protein of Rinderpest virus in transgenic pigeon pea [Cajanus cajan (L.) Millsp.] plants

V. V. Satyavathi, V. Prasad, Abha Khandelwal, M. S. Shaila, G. Lakshmi Sita

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Rinderpest virus is the causative agent of a devastating, often fatal disease in wild and domestic bovids that is endemic in Africa, the Middle East and South Asia. The existing live attenuated vaccine is heat-labile, and thus there is a need for the development of new strategies for vaccination. This paper reports the development of transgenic pigeon pea [Cajanus cajun (L.) Millsp.] expressing one of the protective antigens, the hemagglutinin (H) protein of Rinderpest virus. A 2-kb fragment containing the coding region of the H protein was cloned into pBI121 and mobilized into Agrobacterium tumefaciens strain EHA105. Embryonic axes and cotyledonary nodes from germinated seeds of pigeon pea were used for transformation. The presence of the transo gene in transgenic plants was confirmed by Southern blots, and the specific transcription of the marker gene in the plants was demonstrated by reverse transcription-polymerase chain reaction. Integration of the H gene into the pigeon pea genome was confirmed by Southern hybridization. The expression of the H protein in the transgenic lines was confirmed by Western blot analysis using a polyclonal monospecific antibody to the H protein. The highest level of expression of the hemagglutinin protein in leaves of pigeon pea was 0.49% of the total soluble protein. The transgenic plants were fertile and the transgene expressed in the progeny.

Original languageEnglish
Pages (from-to)651-658
Number of pages8
JournalPlant Cell Reports
Volume21
Issue number7
Publication statusPublished - 01-03-2003
Externally publishedYes

Fingerprint

Rinderpest virus
pigeon peas
Cajanus cajan
hemagglutinins
genetically modified organisms
live vaccines
proteins
Southern blotting
transgenic plants
Cajanus
leaf protein
South Asia
Middle East
Agrobacterium radiobacter
polyclonal antibodies
East Asia
transgenes
Western blotting
genes
transcription (genetics)

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Plant Science

Cite this

Satyavathi, V. V., Prasad, V., Khandelwal, A., Shaila, M. S., & Sita, G. L. (2003). Expression of hemagglutinin protein of Rinderpest virus in transgenic pigeon pea [Cajanus cajan (L.) Millsp.] plants. Plant Cell Reports, 21(7), 651-658.
Satyavathi, V. V. ; Prasad, V. ; Khandelwal, Abha ; Shaila, M. S. ; Sita, G. Lakshmi. / Expression of hemagglutinin protein of Rinderpest virus in transgenic pigeon pea [Cajanus cajan (L.) Millsp.] plants. In: Plant Cell Reports. 2003 ; Vol. 21, No. 7. pp. 651-658.
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Satyavathi, VV, Prasad, V, Khandelwal, A, Shaila, MS & Sita, GL 2003, 'Expression of hemagglutinin protein of Rinderpest virus in transgenic pigeon pea [Cajanus cajan (L.) Millsp.] plants', Plant Cell Reports, vol. 21, no. 7, pp. 651-658.

Expression of hemagglutinin protein of Rinderpest virus in transgenic pigeon pea [Cajanus cajan (L.) Millsp.] plants. / Satyavathi, V. V.; Prasad, V.; Khandelwal, Abha; Shaila, M. S.; Sita, G. Lakshmi.

In: Plant Cell Reports, Vol. 21, No. 7, 01.03.2003, p. 651-658.

Research output: Contribution to journalArticle

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AU - Satyavathi, V. V.

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