Expression of Laminin in oral squamous cell carcinomas

Shweta Yellapurkar, Srikant Natarajan, Karen Boaz, Nidhi Manaktala, Mohan Baliga, Premalatha Shetty, Mukul Prasad, Mahalakshmi Ravi

Research output: Contribution to journalArticle

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Abstract

Background and objectives: Laminin is a significant basement membrane (BM) glycoprotein, the expression of which reflects BM integrity more precisely than do other ECM proteins. The present study aimed to evaluate laminin expression in oral squamous cell carcinomas OSCC and to determine any associations with clinico-pathological parameters (surgical margin status, lymph node involvement, survival and recurrence). Methods: Laminin expression was evaluated in 31 cases of biopsy-proven OSCC by immunohistochemical staining and its association with prognosticators and the Brynes grading system was determined by appropriate statistical analysis. Results: We observed a significant increase in linear staining pattern (p < 0.001) at the tumour-host interface in well-differentiated OSCC cases, in contrast to poorly differentiated lesions which exhibited intense cytoplasmic expression within tumour cells. Higher cytoplasmic laminin expression was seen in 33.3% of cases with involved surgical margins and 69.2% of cases with lymph node metastasis (along with weak/absent staining of laminin around the tumour-host interface - Basement membrane around tumour islands). Similarly, in 60% of the cases who died and in 81.8% of cases with tumour recurrence, moderately intense cytoplasmic laminin expression was seen within tumour cells. On comparing variables of the Brynes grading system, significant cytoplasmic expression of laminin was linked with mild inflammation (p < 0.0016) and increased mitotic activity (p < 0.008). Conclusion: Based on these observations, immunohistochemical expression of laminin might be useful to evaluate histological differentiation and aggressiveness of OSCCs.

Original languageEnglish
Pages (from-to)407-413
Number of pages7
JournalAsian Pacific Journal of Cancer Prevention
Volume19
Issue number2
DOIs
Publication statusPublished - 01-02-2018

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Laminin
Squamous Cell Carcinoma
Basement Membrane
Neoplasms
Staining and Labeling
Lymph Nodes
Recurrence
Membrane Glycoproteins
Islands
Neoplasm Metastasis
Inflammation
Biopsy

All Science Journal Classification (ASJC) codes

  • Epidemiology
  • Oncology
  • Public Health, Environmental and Occupational Health
  • Cancer Research

Cite this

Yellapurkar, Shweta ; Natarajan, Srikant ; Boaz, Karen ; Manaktala, Nidhi ; Baliga, Mohan ; Shetty, Premalatha ; Prasad, Mukul ; Ravi, Mahalakshmi. / Expression of Laminin in oral squamous cell carcinomas. In: Asian Pacific Journal of Cancer Prevention. 2018 ; Vol. 19, No. 2. pp. 407-413.
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abstract = "Background and objectives: Laminin is a significant basement membrane (BM) glycoprotein, the expression of which reflects BM integrity more precisely than do other ECM proteins. The present study aimed to evaluate laminin expression in oral squamous cell carcinomas OSCC and to determine any associations with clinico-pathological parameters (surgical margin status, lymph node involvement, survival and recurrence). Methods: Laminin expression was evaluated in 31 cases of biopsy-proven OSCC by immunohistochemical staining and its association with prognosticators and the Brynes grading system was determined by appropriate statistical analysis. Results: We observed a significant increase in linear staining pattern (p < 0.001) at the tumour-host interface in well-differentiated OSCC cases, in contrast to poorly differentiated lesions which exhibited intense cytoplasmic expression within tumour cells. Higher cytoplasmic laminin expression was seen in 33.3{\%} of cases with involved surgical margins and 69.2{\%} of cases with lymph node metastasis (along with weak/absent staining of laminin around the tumour-host interface - Basement membrane around tumour islands). Similarly, in 60{\%} of the cases who died and in 81.8{\%} of cases with tumour recurrence, moderately intense cytoplasmic laminin expression was seen within tumour cells. On comparing variables of the Brynes grading system, significant cytoplasmic expression of laminin was linked with mild inflammation (p < 0.0016) and increased mitotic activity (p < 0.008). Conclusion: Based on these observations, immunohistochemical expression of laminin might be useful to evaluate histological differentiation and aggressiveness of OSCCs.",
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Expression of Laminin in oral squamous cell carcinomas. / Yellapurkar, Shweta; Natarajan, Srikant; Boaz, Karen; Manaktala, Nidhi; Baliga, Mohan; Shetty, Premalatha; Prasad, Mukul; Ravi, Mahalakshmi.

In: Asian Pacific Journal of Cancer Prevention, Vol. 19, No. 2, 01.02.2018, p. 407-413.

Research output: Contribution to journalArticle

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T1 - Expression of Laminin in oral squamous cell carcinomas

AU - Yellapurkar, Shweta

AU - Natarajan, Srikant

AU - Boaz, Karen

AU - Manaktala, Nidhi

AU - Baliga, Mohan

AU - Shetty, Premalatha

AU - Prasad, Mukul

AU - Ravi, Mahalakshmi

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N2 - Background and objectives: Laminin is a significant basement membrane (BM) glycoprotein, the expression of which reflects BM integrity more precisely than do other ECM proteins. The present study aimed to evaluate laminin expression in oral squamous cell carcinomas OSCC and to determine any associations with clinico-pathological parameters (surgical margin status, lymph node involvement, survival and recurrence). Methods: Laminin expression was evaluated in 31 cases of biopsy-proven OSCC by immunohistochemical staining and its association with prognosticators and the Brynes grading system was determined by appropriate statistical analysis. Results: We observed a significant increase in linear staining pattern (p < 0.001) at the tumour-host interface in well-differentiated OSCC cases, in contrast to poorly differentiated lesions which exhibited intense cytoplasmic expression within tumour cells. Higher cytoplasmic laminin expression was seen in 33.3% of cases with involved surgical margins and 69.2% of cases with lymph node metastasis (along with weak/absent staining of laminin around the tumour-host interface - Basement membrane around tumour islands). Similarly, in 60% of the cases who died and in 81.8% of cases with tumour recurrence, moderately intense cytoplasmic laminin expression was seen within tumour cells. On comparing variables of the Brynes grading system, significant cytoplasmic expression of laminin was linked with mild inflammation (p < 0.0016) and increased mitotic activity (p < 0.008). Conclusion: Based on these observations, immunohistochemical expression of laminin might be useful to evaluate histological differentiation and aggressiveness of OSCCs.

AB - Background and objectives: Laminin is a significant basement membrane (BM) glycoprotein, the expression of which reflects BM integrity more precisely than do other ECM proteins. The present study aimed to evaluate laminin expression in oral squamous cell carcinomas OSCC and to determine any associations with clinico-pathological parameters (surgical margin status, lymph node involvement, survival and recurrence). Methods: Laminin expression was evaluated in 31 cases of biopsy-proven OSCC by immunohistochemical staining and its association with prognosticators and the Brynes grading system was determined by appropriate statistical analysis. Results: We observed a significant increase in linear staining pattern (p < 0.001) at the tumour-host interface in well-differentiated OSCC cases, in contrast to poorly differentiated lesions which exhibited intense cytoplasmic expression within tumour cells. Higher cytoplasmic laminin expression was seen in 33.3% of cases with involved surgical margins and 69.2% of cases with lymph node metastasis (along with weak/absent staining of laminin around the tumour-host interface - Basement membrane around tumour islands). Similarly, in 60% of the cases who died and in 81.8% of cases with tumour recurrence, moderately intense cytoplasmic laminin expression was seen within tumour cells. On comparing variables of the Brynes grading system, significant cytoplasmic expression of laminin was linked with mild inflammation (p < 0.0016) and increased mitotic activity (p < 0.008). Conclusion: Based on these observations, immunohistochemical expression of laminin might be useful to evaluate histological differentiation and aggressiveness of OSCCs.

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