Expression of tenascin and nucleolar organizer region in ameloblastoma and ameloblastic fibroma

Sunitha Carnelio, Hitesh Vij

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

BACKGROUND: The aim of this study was to assess the expression, distribution and comparison of tenascin, a glycoprotein of the extracellular matrix in ameloblastoma and ameloblastic fibroma, both odontogenic neoplasms with diverse biological behavior and to understand the proliferative activity by using the morphometric analysis. METHODS: Paraffin embedded tissue from 25 cases of odontogenic tumors i.e., ameloblastoma (n = 15) and ameloblastic fibroma (n = 10) were used. The expression of tenascin was evaluated using immunohistochemistry. Morphometric analysis of nucleolar organizer regions (NORs) from ameloblastoma and ameloblastic fibroma was carried out by silver staining. RESULTS: A heterogeneous expression of tenascin was found in ameloblastoma which was mainly localized at the epithelialmesenchymal interface and a patchy distribution was observed in the stroma (80%), while strong positivity was observed in the stroma and at the basement membrane zone of ameloblastic fibroma (100%). argyrophilic nucleolar organizer regions (AgNORs) revealed higher mean counts in ameloblastoma (3.093 ± 0.902) when compared with those of ameloblastic fibroma (1.553 ± 0.250). Ameloblastoma presented more than two NORs (two to five) per nucleus in majority of the cells, while ameloblastic fibroma exhibited only one NORs per nucleus. CONCLUSIONS: Expression of tenascin in these neoplasms suggest that it could play a role in epithelialmesenchymal interaction, while AgNORs reveal that ameloblastomas are more aggressive when compared with ameloblastic fibromas.

Original languageEnglish
Pages (from-to)223-229
Number of pages7
JournalJournal of Oral Pathology and Medicine
Volume39
Issue number3
DOIs
Publication statusPublished - 01-03-2010

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Ameloblastoma
Nucleolus Organizer Region
Tenascin
Fibroma
Odontogenic Tumors
Silver Staining
Basement Membrane
Paraffin
Extracellular Matrix
Neoplasms
Glycoproteins
Immunohistochemistry

All Science Journal Classification (ASJC) codes

  • Cancer Research
  • Pathology and Forensic Medicine
  • Otorhinolaryngology
  • Oral Surgery
  • Periodontics

Cite this

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title = "Expression of tenascin and nucleolar organizer region in ameloblastoma and ameloblastic fibroma",
abstract = "BACKGROUND: The aim of this study was to assess the expression, distribution and comparison of tenascin, a glycoprotein of the extracellular matrix in ameloblastoma and ameloblastic fibroma, both odontogenic neoplasms with diverse biological behavior and to understand the proliferative activity by using the morphometric analysis. METHODS: Paraffin embedded tissue from 25 cases of odontogenic tumors i.e., ameloblastoma (n = 15) and ameloblastic fibroma (n = 10) were used. The expression of tenascin was evaluated using immunohistochemistry. Morphometric analysis of nucleolar organizer regions (NORs) from ameloblastoma and ameloblastic fibroma was carried out by silver staining. RESULTS: A heterogeneous expression of tenascin was found in ameloblastoma which was mainly localized at the epithelialmesenchymal interface and a patchy distribution was observed in the stroma (80{\%}), while strong positivity was observed in the stroma and at the basement membrane zone of ameloblastic fibroma (100{\%}). argyrophilic nucleolar organizer regions (AgNORs) revealed higher mean counts in ameloblastoma (3.093 ± 0.902) when compared with those of ameloblastic fibroma (1.553 ± 0.250). Ameloblastoma presented more than two NORs (two to five) per nucleus in majority of the cells, while ameloblastic fibroma exhibited only one NORs per nucleus. CONCLUSIONS: Expression of tenascin in these neoplasms suggest that it could play a role in epithelialmesenchymal interaction, while AgNORs reveal that ameloblastomas are more aggressive when compared with ameloblastic fibromas.",
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Expression of tenascin and nucleolar organizer region in ameloblastoma and ameloblastic fibroma. / Carnelio, Sunitha; Vij, Hitesh.

In: Journal of Oral Pathology and Medicine, Vol. 39, No. 3, 01.03.2010, p. 223-229.

Research output: Contribution to journalArticle

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