Expression studies of Bacillus licheniformis chitin deacetylase in E. coli Rosetta cells

Ritu Raval, Robin Simsa, Keyur Raval

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Chitin, the biopolymer of the N-acetylglucosamine, is the most abundant biopolymer on the planet after cellulose. However owing to its crystalline nature, its deacetylated derivative; chitosan is industrially more potent. This conversion on an enzymatic scale can be made using chitin deacetylase. The metagenomics library constructed from the soil exposed to chitin and chitosan yielded chitin modifying enzymes, one of them being chitin deacetylase (CDA) utilized for the present study. The gene was amplified and expressed using the pET 22b vector in E. coli Rosetta cells. The effect of two additives; chitin and glycerol on the CDA activity were studied. The inclusion of glycerol in the medium improved the biomass by 50% from the initial value of 1.25 g/l to 2.5 g/l. The activity of CDA increased from 90 μmol/min/ml to 343 μmol/min/ml. The CDA activity reported in the present paper is the highest observed for any strain. The addition of glycerol to the media not only helped improve the yield of the chitin deacetylase but also imparted value addition to the waste of the biofuel industry.

Original languageEnglish
Pages (from-to)1692-1696
Number of pages5
JournalInternational Journal of Biological Macromolecules
Volume104
DOIs
Publication statusPublished - 01-11-2017

Fingerprint

chitin deacetylase
Bacilli
Escherichia coli
Chitin
Glycerol
Biopolymers
Chitosan
Planets
Metagenomics
Acetylglucosamine
Biofuels
Cellulose
Biomass
Bacillus licheniformis
Industry
Soil
Genes
Crystalline materials
Derivatives

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biochemistry
  • Molecular Biology

Cite this

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abstract = "Chitin, the biopolymer of the N-acetylglucosamine, is the most abundant biopolymer on the planet after cellulose. However owing to its crystalline nature, its deacetylated derivative; chitosan is industrially more potent. This conversion on an enzymatic scale can be made using chitin deacetylase. The metagenomics library constructed from the soil exposed to chitin and chitosan yielded chitin modifying enzymes, one of them being chitin deacetylase (CDA) utilized for the present study. The gene was amplified and expressed using the pET 22b vector in E. coli Rosetta cells. The effect of two additives; chitin and glycerol on the CDA activity were studied. The inclusion of glycerol in the medium improved the biomass by 50{\%} from the initial value of 1.25 g/l to 2.5 g/l. The activity of CDA increased from 90 μmol/min/ml to 343 μmol/min/ml. The CDA activity reported in the present paper is the highest observed for any strain. The addition of glycerol to the media not only helped improve the yield of the chitin deacetylase but also imparted value addition to the waste of the biofuel industry.",
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Expression studies of Bacillus licheniformis chitin deacetylase in E. coli Rosetta cells. / Raval, Ritu; Simsa, Robin; Raval, Keyur.

In: International Journal of Biological Macromolecules, Vol. 104, 01.11.2017, p. 1692-1696.

Research output: Contribution to journalArticle

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