TY - JOUR
T1 - Factorial design-based fabrication of biopolymer-functionalized Asiatic acid-embedded liposomes
T2 - in-vitro characterization and evaluation
AU - Dhas, Namdev
AU - Preetha, Hosadurga Shantharam
AU - Dubey, Akhilesh
AU - Ravi, Gundawar
AU - Govindan, Induja
AU - Rama, Annamalai
AU - Naha, Anup
AU - Hebbar, Srinivas
N1 - Funding Information:
The authors would like to thank the support of Manipal College of Pharmaceutical Sciences and NGSM Institute of Pharmaceutical Sciences.
Publisher Copyright:
© 2022 Namdev Dhas et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/).
PY - 2022/11
Y1 - 2022/11
N2 - This study investigated the in-vitro comparison between Asiatic acid (AA) liposomes and surface-modified liposomes of AA (coated by chitosan and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-methoxy(polyethylene glycol (DSPE MPEG) separately) to treat Alzheimer’s disease. The optimized formula was obtained using Design-Expert software, and liposomes were formulated as per the thin-film hydration method. Chitosan-coated AA liposomes and stealth AA liposomes were fabricated using electrostatic interaction. The prepared formulations were evaluated for compatibility, liposomal vesicle size, drug entrapment, drug content, dispersibility index, and surface morphology (transmission electron microscopy and atomic force microscopy). Compared to AA, AA liposome (AAL), and stealth AA liposome (SAAL) demonstrated sustained-release and improved drug release rates, with 97.00 ± 0.56% and 86.42 ± 0.38% released in 18 hours, respectively. Chitosan-coated AA liposome (CAAL) showed an 85.45 ± 0.43%, better than the drug release rate in 24 hours. The ex -vivo AA permeation from CAAL was better than the other two forms of liposomes. The stability data, which signifies the vesicle size of the liposomes, drug content, and %EE of CAAL and SAAL, were consistent and showed that CAAL was more stable in simulated gastric fluid. This study suggested that chitosan-coated AA liposomes showed better stability and sustained drug release than AAL and SAAL.
AB - This study investigated the in-vitro comparison between Asiatic acid (AA) liposomes and surface-modified liposomes of AA (coated by chitosan and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-methoxy(polyethylene glycol (DSPE MPEG) separately) to treat Alzheimer’s disease. The optimized formula was obtained using Design-Expert software, and liposomes were formulated as per the thin-film hydration method. Chitosan-coated AA liposomes and stealth AA liposomes were fabricated using electrostatic interaction. The prepared formulations were evaluated for compatibility, liposomal vesicle size, drug entrapment, drug content, dispersibility index, and surface morphology (transmission electron microscopy and atomic force microscopy). Compared to AA, AA liposome (AAL), and stealth AA liposome (SAAL) demonstrated sustained-release and improved drug release rates, with 97.00 ± 0.56% and 86.42 ± 0.38% released in 18 hours, respectively. Chitosan-coated AA liposome (CAAL) showed an 85.45 ± 0.43%, better than the drug release rate in 24 hours. The ex -vivo AA permeation from CAAL was better than the other two forms of liposomes. The stability data, which signifies the vesicle size of the liposomes, drug content, and %EE of CAAL and SAAL, were consistent and showed that CAAL was more stable in simulated gastric fluid. This study suggested that chitosan-coated AA liposomes showed better stability and sustained drug release than AAL and SAAL.
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U2 - 10.7324/JAPS.2022.121108
DO - 10.7324/JAPS.2022.121108
M3 - Article
AN - SCOPUS:85142256238
VL - 12
SP - 71
EP - 81
JO - Journal of Applied Pharmaceutical Science
JF - Journal of Applied Pharmaceutical Science
SN - 2231-3354
IS - 11
ER -