Factorial design-based fabrication of biopolymer-functionalized Asiatic acid-embedded liposomes: in-vitro characterization and evaluation

Namdev Dhas, Hosadurga Shantharam Preetha, Akhilesh Dubey, Gundawar Ravi, Induja Govindan, Annamalai Rama, Anup Naha, Srinivas Hebbar

Research output: Contribution to journalArticlepeer-review

Abstract

This study investigated the in-vitro comparison between Asiatic acid (AA) liposomes and surface-modified liposomes of AA (coated by chitosan and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-methoxy(polyethylene glycol (DSPE MPEG) separately) to treat Alzheimer’s disease. The optimized formula was obtained using Design-Expert software, and liposomes were formulated as per the thin-film hydration method. Chitosan-coated AA liposomes and stealth AA liposomes were fabricated using electrostatic interaction. The prepared formulations were evaluated for compatibility, liposomal vesicle size, drug entrapment, drug content, dispersibility index, and surface morphology (transmission electron microscopy and atomic force microscopy). Compared to AA, AA liposome (AAL), and stealth AA liposome (SAAL) demonstrated sustained-release and improved drug release rates, with 97.00 ± 0.56% and 86.42 ± 0.38% released in 18 hours, respectively. Chitosan-coated AA liposome (CAAL) showed an 85.45 ± 0.43%, better than the drug release rate in 24 hours. The ex -vivo AA permeation from CAAL was better than the other two forms of liposomes. The stability data, which signifies the vesicle size of the liposomes, drug content, and %EE of CAAL and SAAL, were consistent and showed that CAAL was more stable in simulated gastric fluid. This study suggested that chitosan-coated AA liposomes showed better stability and sustained drug release than AAL and SAAL.

Original languageEnglish
Pages (from-to)71-81
Number of pages11
JournalJournal of Applied Pharmaceutical Science
Volume12
Issue number11
DOIs
Publication statusPublished - 11-2022

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Pharmacology (medical)

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