Fetal central nervous system and thymic alterations following cyclophosphamide treatment of pregnant mice

Prakash, Gajendra Singh, Sukh Mahendra Singh

Research output: Contribution to journalArticle

Abstract

The present study assessed central nervous system (CNS) and immune system changes in murine fetuses after cyclophosphamide (CP) exposure during intrauterine life. A single CP dose of 0, 10, or 20mg/kg body weight was administered by intraperitoneal injection to pregnant mice (20/group) on day 11 of gestation (GD 11) and fetuses were evaluated on day 19 of gestation (GD 19). Fetuses were examined for external changes, and then the brains and thymuses were removed for further evaluations of histological changes, protein content, apoptotic cell count, DNA fragmentation, and in vitro cell proliferation using 1 fetus/litter for each assessment. Brains and thymuses from CP-exposed fetuses were smaller in size and distorted in overall shape compared to those from the control group. Estimated mean protein content (mg/mL) of brains was decreased in the CP-exposed groups. In both brain cells and thymocytes there was an increase in mean apoptotic cell counts and in mean percent DNA fragmentation in the exposed groups. The in vitro cell proliferation assays conducted with cells from exposed fetuses exhibited a mean decrease in the number of both brain cells and thymocytes generated. These findings indicate that maternal CP treatment on GD 11 in mice results in marked fetal toxicity characterized by reduced live litter size, fetal body weights as well as brain and thymic weights and malformations which are accompanied by changes in brain protein content, brain and thymic apoptosis, DNA fragmentation and in vitro cell proliferation at term.

Original languageEnglish
Pages (from-to)775-787
Number of pages13
JournalInternational Journal of Morphology
Volume25
Issue number4
Publication statusPublished - 01-12-2007
Externally publishedYes

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Cyclophosphamide
Central Nervous System
Fetus
Brain
DNA Fragmentation
Cell Proliferation
Thymocytes
Therapeutics
Thymus Gland
Cell Count
Pregnancy
Litter Size
Fetal Weight
Proteins
Intraperitoneal Injections
Immune System
Body Weight
Mothers
Apoptosis
Weights and Measures

All Science Journal Classification (ASJC) codes

  • Anatomy

Cite this

Prakash ; Singh, Gajendra ; Singh, Sukh Mahendra. / Fetal central nervous system and thymic alterations following cyclophosphamide treatment of pregnant mice. In: International Journal of Morphology. 2007 ; Vol. 25, No. 4. pp. 775-787.
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Fetal central nervous system and thymic alterations following cyclophosphamide treatment of pregnant mice. / Prakash; Singh, Gajendra; Singh, Sukh Mahendra.

In: International Journal of Morphology, Vol. 25, No. 4, 01.12.2007, p. 775-787.

Research output: Contribution to journalArticle

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