Ficus racemosa stem bark extract: A potent antioxidant and a probable natural radioprotector

V. P. Veerapur, K. R. Prabhakar, Vipan Kumar Parihar, Machendar Reddy Kandadi, S. Ramakrishana, B. Mishra, B. S. Satish Rao, K. K. Srinivasan, K. I. Priyadarsini, M. K. Unnikrishnan

Research output: Contribution to journalArticlepeer-review

74 Citations (Scopus)

Abstract

Ethanol extract (FRE) and water extract (FRW) of Ficus racemosa (family: Moraceae) were subjected to free radical scavenging both by steady state and time resolved methods such as nanosecond pulse radiolysis and stopped-flow spectrophotometric analyses. FRE exhibited significantly higher steady state antioxidant activity than FRW. FRE exhibited concentration dependent DPPH, ABTS•-, hydroxyl radical and superoxide radical scavenging and inhibition of lipid peroxidation with IC50 comparable with tested standard compounds. In vitro radioprotective potential of FRE was studied using micronucleus assay in irradiated Chinese hamster lung fibroblast cells (V79). Pretreatment with different doses of FRE 1h prior to 2 Gy γ-radiation resulted in a significant (P < 0.001) decrease in the percentage of micronucleated binuclear V79 cells. Maximum radioprotection was observed at 20 μg/ml of FRE. The radioprotection was found to be significant (P < 0.01) when cells were treated with optimum dose of FRE (20 μg/ml) 1 h prior to 0.5, 1, 2, 3 and 4 Gy γ-irradiation compared to the respective radiation controls. The cytokinesis-block proliferative index indicated that FRE does not alter radiation induced cell cycle delay. Based on all these results we conclude that the ethanol extract of F. racemosa acts as a potent antioxidant and a probable radioprotector.

Original languageEnglish
Pages (from-to)317-324
Number of pages8
JournalEvidence-based Complementary and Alternative Medicine
Volume6
Issue number3
DOIs
Publication statusPublished - 09-2009

All Science Journal Classification (ASJC) codes

  • Complementary and alternative medicine

Fingerprint Dive into the research topics of 'Ficus racemosa stem bark extract: A potent antioxidant and a probable natural radioprotector'. Together they form a unique fingerprint.

Cite this