Ficus racemosa stem bark extract: A potent antioxidant and a probable natural radioprotector

V. P. Veerapur, K. R. Prabhakar, Vipan Kumar Parihar, Machendar Reddy Kandadi, S. Ramakrishana, B. Mishra, B. S. Satish Rao, K. K. Srinivasan, K. I. Priyadarsini, M. K. Unnikrishnan

Research output: Contribution to journalArticle

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Abstract

Ethanol extract (FRE) and water extract (FRW) of Ficus racemosa (family: Moraceae) were subjected to free radical scavenging both by steady state and time resolved methods such as nanosecond pulse radiolysis and stopped-flow spectrophotometric analyses. FRE exhibited significantly higher steady state antioxidant activity than FRW. FRE exhibited concentration dependent DPPH, ABTS•-, hydroxyl radical and superoxide radical scavenging and inhibition of lipid peroxidation with IC50 comparable with tested standard compounds. In vitro radioprotective potential of FRE was studied using micronucleus assay in irradiated Chinese hamster lung fibroblast cells (V79). Pretreatment with different doses of FRE 1h prior to 2 Gy γ-radiation resulted in a significant (P < 0.001) decrease in the percentage of micronucleated binuclear V79 cells. Maximum radioprotection was observed at 20 μg/ml of FRE. The radioprotection was found to be significant (P < 0.01) when cells were treated with optimum dose of FRE (20 μg/ml) 1 h prior to 0.5, 1, 2, 3 and 4 Gy γ-irradiation compared to the respective radiation controls. The cytokinesis-block proliferative index indicated that FRE does not alter radiation induced cell cycle delay. Based on all these results we conclude that the ethanol extract of F. racemosa acts as a potent antioxidant and a probable radioprotector.

Original languageEnglish
Pages (from-to)317-324
Number of pages8
JournalEvidence-based Complementary and Alternative Medicine
Volume6
Issue number3
DOIs
Publication statusPublished - 09-2009

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Ficus
Antioxidants
Ethanol
Moraceae
Pulse Radiolysis
Radiation
Radiation Dosage
Micronucleus Tests
Cytokinesis
Water
Cricetulus
Superoxides
Hydroxyl Radical
Lipid Peroxidation
Inhibitory Concentration 50
Free Radicals
Cell Cycle
Fibroblasts
Lung

All Science Journal Classification (ASJC) codes

  • Complementary and alternative medicine

Cite this

Veerapur, V. P., Prabhakar, K. R., Parihar, V. K., Kandadi, M. R., Ramakrishana, S., Mishra, B., ... Unnikrishnan, M. K. (2009). Ficus racemosa stem bark extract: A potent antioxidant and a probable natural radioprotector. Evidence-based Complementary and Alternative Medicine, 6(3), 317-324. https://doi.org/10.1093/ecam/nem119
Veerapur, V. P. ; Prabhakar, K. R. ; Parihar, Vipan Kumar ; Kandadi, Machendar Reddy ; Ramakrishana, S. ; Mishra, B. ; Satish Rao, B. S. ; Srinivasan, K. K. ; Priyadarsini, K. I. ; Unnikrishnan, M. K. / Ficus racemosa stem bark extract : A potent antioxidant and a probable natural radioprotector. In: Evidence-based Complementary and Alternative Medicine. 2009 ; Vol. 6, No. 3. pp. 317-324.
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Veerapur, VP, Prabhakar, KR, Parihar, VK, Kandadi, MR, Ramakrishana, S, Mishra, B, Satish Rao, BS, Srinivasan, KK, Priyadarsini, KI & Unnikrishnan, MK 2009, 'Ficus racemosa stem bark extract: A potent antioxidant and a probable natural radioprotector', Evidence-based Complementary and Alternative Medicine, vol. 6, no. 3, pp. 317-324. https://doi.org/10.1093/ecam/nem119

Ficus racemosa stem bark extract : A potent antioxidant and a probable natural radioprotector. / Veerapur, V. P.; Prabhakar, K. R.; Parihar, Vipan Kumar; Kandadi, Machendar Reddy; Ramakrishana, S.; Mishra, B.; Satish Rao, B. S.; Srinivasan, K. K.; Priyadarsini, K. I.; Unnikrishnan, M. K.

In: Evidence-based Complementary and Alternative Medicine, Vol. 6, No. 3, 09.2009, p. 317-324.

Research output: Contribution to journalArticle

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T1 - Ficus racemosa stem bark extract

T2 - A potent antioxidant and a probable natural radioprotector

AU - Veerapur, V. P.

AU - Prabhakar, K. R.

AU - Parihar, Vipan Kumar

AU - Kandadi, Machendar Reddy

AU - Ramakrishana, S.

AU - Mishra, B.

AU - Satish Rao, B. S.

AU - Srinivasan, K. K.

AU - Priyadarsini, K. I.

AU - Unnikrishnan, M. K.

PY - 2009/9

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