Fish Meal Extract Bile Esculin Agar (FMBE) A Selective Medium for Bacteroides Fragilis Group

V. K. Beena, Sugandhi Rao, Mohan Kotian, P. G. Shivananda

Research output: Contribution to journalArticle

Abstract

Fish meal extract Bile Esculin agar (FMBE) is prepared using Fish meal extract concentrate as the basal substance, for the selective isolation and presumptive identification of B.fragilis group. The efficiency of the medium was evaluated by growing stock cultures of B.fragilis groups as well as inoculating clinical specimens and comparing the results with Bacteroides bile esculin agar (BBE). All the 87 stock cultures of B.fragilis grew on FMBE and BBE. No other anaerobes tested grew on the medium. However 7 out of 65 neomycin resistant aerobes grew on the FMBE. From the 100 clinical samples, 62 strains of B. Fragilis group were recovered on FMBE and BBE, and 53 strains on supplemented BHIBA. The cost effectiveness, selectivity and the ability to detect esculin hydrolysis will enable FMBE as a suitable medium as comparable to that of BBE, if not superior.

Original languageEnglish
Pages (from-to)361-364
Number of pages4
JournalIndian Journal of Pathology and Microbiology
Volume40
Issue number3
Publication statusPublished - 01-12-1997
Externally publishedYes

Fingerprint

Esculin
Bacteroides fragilis
Bile
Agar
Meals
Fishes
Bacteroides
Neomycin
Cost-Benefit Analysis
Hydrolysis

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Microbiology (medical)

Cite this

Beena, V. K. ; Rao, Sugandhi ; Kotian, Mohan ; Shivananda, P. G. / Fish Meal Extract Bile Esculin Agar (FMBE) A Selective Medium for Bacteroides Fragilis Group. In: Indian Journal of Pathology and Microbiology. 1997 ; Vol. 40, No. 3. pp. 361-364.
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Fish Meal Extract Bile Esculin Agar (FMBE) A Selective Medium for Bacteroides Fragilis Group. / Beena, V. K.; Rao, Sugandhi; Kotian, Mohan; Shivananda, P. G.

In: Indian Journal of Pathology and Microbiology, Vol. 40, No. 3, 01.12.1997, p. 361-364.

Research output: Contribution to journalArticle

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