Fishmeal extract bile salt lactose agar - A differential medium for enteric bacteria

K. Subbannayya, J. Udayalaxmi, M. Anugraha

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Fishmeal extract bile salt lactose agar (FEBLA), a new differential medium for enteric bacteria was developed and evaluated for its ability to grow and differentiate lactose fermenters (LF) from non-lactose fermenters (NLF) in comparison with MacConkeys agar. Performance of FEBLA was at par with the latter. On FEBLA medium, the contrast between LF and NLF colonies was pronounced and Klebsiella pneumoniae produced more mucoid colonies than on MacConkeys agar (Hi Media). Unlike MacConkeys agar, a 24 h culture of K. pneumoniae cells on FEBLA were longer and thicker with abundant capsular material around the bacilli. Eschericliia coli produced long and thick cells but only after 48h. No change in cell morphology was evident with regard to Salmonella typhi, S. paratyphi A, Shigella flexneri, Pseudomonas aeruginosa, Proteus mirabilis, Proteus vulgaris, Citrobacter koseri and Acinetobacter baumannii. Performance of the medium was controlled using E. coli and S. flexneri. FEBLA is simple, cost effective and may be a suitable alternative in the preliminary identification of enteric bacteria.

Original languageEnglish
Pages (from-to)675-678
Number of pages4
JournalIndian Journal of Experimental Biology
Volume44
Issue number8
Publication statusPublished - 28-12-2006

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Enterobacteriaceae
Lactose
Bile Acids and Salts
Agar
Shigella flexneri
Klebsiella pneumoniae
Citrobacter koseri
Proteus vulgaris
Acinetobacter baumannii
Proteus mirabilis
Salmonella typhi
Pseudomonas aeruginosa
Bacillus
Escherichia coli
Costs and Cost Analysis

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Molecular Biology
  • Cell Biology

Cite this

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Fishmeal extract bile salt lactose agar - A differential medium for enteric bacteria. / Subbannayya, K.; Udayalaxmi, J.; Anugraha, M.

In: Indian Journal of Experimental Biology, Vol. 44, No. 8, 28.12.2006, p. 675-678.

Research output: Contribution to journalArticle

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