Fluorescence and circular dichroism studies on binding and conformational aspects of an anti-leukemic drug with DNA

Ashwini H. Hegde, J. Seetharamappa

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

In the present study, we have explored the mode of binding of an anti-leukemic drug, imatinib (IMT) mesylate with DNA and resulting conformational changes in DNA double helix. UV-Vis absorption, fluorescence and circular dichroism spectroscopic techniques were employed to study these interactions. Spectroscopic results revealed that the intercalation was the primary mode of interaction between IMT and DNA. The binding constant value of 6.62 × 103 M-1 indicated the moderate interaction between IMT and DNA. Melting temperature of DNA increased from 75 to 80 °C upon interaction with IMT.

Original languageEnglish
Pages (from-to)67-71
Number of pages5
JournalMolecular Biology Reports
Volume41
Issue number1
DOIs
Publication statusPublished - 01-01-2014

Fingerprint

Circular Dichroism
Fluorescence
DNA
Pharmaceutical Preparations
Nucleic Acid Denaturation
Temperature
Imatinib Mesylate

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

@article{296e0f4dffc24dcd88b48aaf43afd114,
title = "Fluorescence and circular dichroism studies on binding and conformational aspects of an anti-leukemic drug with DNA",
abstract = "In the present study, we have explored the mode of binding of an anti-leukemic drug, imatinib (IMT) mesylate with DNA and resulting conformational changes in DNA double helix. UV-Vis absorption, fluorescence and circular dichroism spectroscopic techniques were employed to study these interactions. Spectroscopic results revealed that the intercalation was the primary mode of interaction between IMT and DNA. The binding constant value of 6.62 × 103 M-1 indicated the moderate interaction between IMT and DNA. Melting temperature of DNA increased from 75 to 80 °C upon interaction with IMT.",
author = "Hegde, {Ashwini H.} and J. Seetharamappa",
year = "2014",
month = "1",
day = "1",
doi = "10.1007/s11033-013-2838-2",
language = "English",
volume = "41",
pages = "67--71",
journal = "Molecular Biology Reports",
issn = "0301-4851",
publisher = "Springer Netherlands",
number = "1",

}

Fluorescence and circular dichroism studies on binding and conformational aspects of an anti-leukemic drug with DNA. / Hegde, Ashwini H.; Seetharamappa, J.

In: Molecular Biology Reports, Vol. 41, No. 1, 01.01.2014, p. 67-71.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Fluorescence and circular dichroism studies on binding and conformational aspects of an anti-leukemic drug with DNA

AU - Hegde, Ashwini H.

AU - Seetharamappa, J.

PY - 2014/1/1

Y1 - 2014/1/1

N2 - In the present study, we have explored the mode of binding of an anti-leukemic drug, imatinib (IMT) mesylate with DNA and resulting conformational changes in DNA double helix. UV-Vis absorption, fluorescence and circular dichroism spectroscopic techniques were employed to study these interactions. Spectroscopic results revealed that the intercalation was the primary mode of interaction between IMT and DNA. The binding constant value of 6.62 × 103 M-1 indicated the moderate interaction between IMT and DNA. Melting temperature of DNA increased from 75 to 80 °C upon interaction with IMT.

AB - In the present study, we have explored the mode of binding of an anti-leukemic drug, imatinib (IMT) mesylate with DNA and resulting conformational changes in DNA double helix. UV-Vis absorption, fluorescence and circular dichroism spectroscopic techniques were employed to study these interactions. Spectroscopic results revealed that the intercalation was the primary mode of interaction between IMT and DNA. The binding constant value of 6.62 × 103 M-1 indicated the moderate interaction between IMT and DNA. Melting temperature of DNA increased from 75 to 80 °C upon interaction with IMT.

UR - http://www.scopus.com/inward/record.url?scp=84891933430&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84891933430&partnerID=8YFLogxK

U2 - 10.1007/s11033-013-2838-2

DO - 10.1007/s11033-013-2838-2

M3 - Article

VL - 41

SP - 67

EP - 71

JO - Molecular Biology Reports

JF - Molecular Biology Reports

SN - 0301-4851

IS - 1

ER -