Fluorescence lifetime imaging with pulsed diode laser enabled stimulated emission

Jianhong Ge; Yixin Wang; Cuifang Kuang; Shin Shian Lee; Nirmal Mazumder; Fu Jen Kao

doi:10.1117/12.2009387

Fluorescence lifetime imaging with pulsed diode laser enabled stimulated emission

Jianhong Ge, Yixin Wang, Cuifang Kuang, Shin Shian Lee, Nirmal Mazumder, Fu Jen Kao

Department of Biophysics, Manipal School of Life Sciences, Manipal

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We present here a stimulated emission based fluorescence lifetime imaging (FLIM) scheme using a pair of synchronized diode lasers operating at gain switched pulse mode. The two semiconductor lasers, with wavelengths at 635 nm and 700 nm, serve as the excitation and the stimulation light sources for the ATTO647N labeled sample, respectively. FLIM is readily achieved with their relative time delay controlled electronically. The coherent nature of the stimulated emission signal also allows FLIM at long working distance. In this way, a high performance all-semiconductor FLIM module is realized in a flexible, compact, and cost effective configuration.

Original language	English
Title of host publication	Multiphoton Microscopy in the Biomedical Sciences XIII
Volume	8588
DOIs	https://doi.org/10.1117/12.2009387
Publication status	Published - 2013
Event	Multiphoton Microscopy in the Biomedical Sciences XIII - San Francisco, CA, United States Duration: 03-02-2013 → 05-02-2013

Conference

Conference	Multiphoton Microscopy in the Biomedical Sciences XIII
Country/Territory	United States
City	San Francisco, CA
Period	03-02-13 → 05-02-13

All Science Journal Classification (ASJC) codes

Atomic and Molecular Physics, and Optics
Electronic, Optical and Magnetic Materials
Biomaterials
Radiology Nuclear Medicine and imaging

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10.1117/12.2009387

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title = "Fluorescence lifetime imaging with pulsed diode laser enabled stimulated emission",

abstract = "We present here a stimulated emission based fluorescence lifetime imaging (FLIM) scheme using a pair of synchronized diode lasers operating at gain switched pulse mode. The two semiconductor lasers, with wavelengths at 635 nm and 700 nm, serve as the excitation and the stimulation light sources for the ATTO647N labeled sample, respectively. FLIM is readily achieved with their relative time delay controlled electronically. The coherent nature of the stimulated emission signal also allows FLIM at long working distance. In this way, a high performance all-semiconductor FLIM module is realized in a flexible, compact, and cost effective configuration.",

author = "Jianhong Ge and Yixin Wang and Cuifang Kuang and Lee, {Shin Shian} and Nirmal Mazumder and Kao, {Fu Jen}",

year = "2013",

doi = "10.1117/12.2009387",

language = "English",

isbn = "9780819493576",

volume = "8588",

booktitle = "Multiphoton Microscopy in the Biomedical Sciences XIII",

note = "Multiphoton Microscopy in the Biomedical Sciences XIII ; Conference date: 03-02-2013 Through 05-02-2013",

}

TY - GEN

T1 - Fluorescence lifetime imaging with pulsed diode laser enabled stimulated emission

AU - Ge, Jianhong

AU - Wang, Yixin

AU - Kuang, Cuifang

AU - Lee, Shin Shian

AU - Mazumder, Nirmal

AU - Kao, Fu Jen

PY - 2013

Y1 - 2013

N2 - We present here a stimulated emission based fluorescence lifetime imaging (FLIM) scheme using a pair of synchronized diode lasers operating at gain switched pulse mode. The two semiconductor lasers, with wavelengths at 635 nm and 700 nm, serve as the excitation and the stimulation light sources for the ATTO647N labeled sample, respectively. FLIM is readily achieved with their relative time delay controlled electronically. The coherent nature of the stimulated emission signal also allows FLIM at long working distance. In this way, a high performance all-semiconductor FLIM module is realized in a flexible, compact, and cost effective configuration.

AB - We present here a stimulated emission based fluorescence lifetime imaging (FLIM) scheme using a pair of synchronized diode lasers operating at gain switched pulse mode. The two semiconductor lasers, with wavelengths at 635 nm and 700 nm, serve as the excitation and the stimulation light sources for the ATTO647N labeled sample, respectively. FLIM is readily achieved with their relative time delay controlled electronically. The coherent nature of the stimulated emission signal also allows FLIM at long working distance. In this way, a high performance all-semiconductor FLIM module is realized in a flexible, compact, and cost effective configuration.

UR - http://www.scopus.com/inward/record.url?scp=84878706564&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84878706564&partnerID=8YFLogxK

U2 - 10.1117/12.2009387

DO - 10.1117/12.2009387

M3 - Conference contribution

AN - SCOPUS:84878706564

SN - 9780819493576

VL - 8588

BT - Multiphoton Microscopy in the Biomedical Sciences XIII

T2 - Multiphoton Microscopy in the Biomedical Sciences XIII

Y2 - 3 February 2013 through 5 February 2013

ER -

Fluorescence lifetime imaging with pulsed diode laser enabled stimulated emission

Abstract

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All Science Journal Classification (ASJC) codes

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