Fluorescence lifetime imaging with pulsed diode laser enabled stimulated emission

Jianhong Ge, Yixin Wang, Cuifang Kuang, Shin Shian Lee, Nirmal Mazumder, Fu Jen Kao

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present here a stimulated emission based fluorescence lifetime imaging (FLIM) scheme using a pair of synchronized diode lasers operating at gain switched pulse mode. The two semiconductor lasers, with wavelengths at 635 nm and 700 nm, serve as the excitation and the stimulation light sources for the ATTO647N labeled sample, respectively. FLIM is readily achieved with their relative time delay controlled electronically. The coherent nature of the stimulated emission signal also allows FLIM at long working distance. In this way, a high performance all-semiconductor FLIM module is realized in a flexible, compact, and cost effective configuration.

Original languageEnglish
Title of host publicationMultiphoton Microscopy in the Biomedical Sciences XIII
Volume8588
DOIs
Publication statusPublished - 2013
EventMultiphoton Microscopy in the Biomedical Sciences XIII - San Francisco, CA, United States
Duration: 03-02-201305-02-2013

Conference

ConferenceMultiphoton Microscopy in the Biomedical Sciences XIII
CountryUnited States
CitySan Francisco, CA
Period03-02-1305-02-13

Fingerprint

Semiconductor Lasers
Stimulated emission
Optical Imaging
stimulated emission
Semiconductor lasers
Lasers
Fluorescence
semiconductor lasers
Imaging techniques
life (durability)
fluorescence
Semiconductors
stimulation
Light sources
Time delay
light sources
time lag
modules
Semiconductor materials
costs

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Ge, J., Wang, Y., Kuang, C., Lee, S. S., Mazumder, N., & Kao, F. J. (2013). Fluorescence lifetime imaging with pulsed diode laser enabled stimulated emission. In Multiphoton Microscopy in the Biomedical Sciences XIII (Vol. 8588). [858832] https://doi.org/10.1117/12.2009387
Ge, Jianhong ; Wang, Yixin ; Kuang, Cuifang ; Lee, Shin Shian ; Mazumder, Nirmal ; Kao, Fu Jen. / Fluorescence lifetime imaging with pulsed diode laser enabled stimulated emission. Multiphoton Microscopy in the Biomedical Sciences XIII. Vol. 8588 2013.
@inproceedings{3b7dcfd408dd4e4488d55f8488747fe5,
title = "Fluorescence lifetime imaging with pulsed diode laser enabled stimulated emission",
abstract = "We present here a stimulated emission based fluorescence lifetime imaging (FLIM) scheme using a pair of synchronized diode lasers operating at gain switched pulse mode. The two semiconductor lasers, with wavelengths at 635 nm and 700 nm, serve as the excitation and the stimulation light sources for the ATTO647N labeled sample, respectively. FLIM is readily achieved with their relative time delay controlled electronically. The coherent nature of the stimulated emission signal also allows FLIM at long working distance. In this way, a high performance all-semiconductor FLIM module is realized in a flexible, compact, and cost effective configuration.",
author = "Jianhong Ge and Yixin Wang and Cuifang Kuang and Lee, {Shin Shian} and Nirmal Mazumder and Kao, {Fu Jen}",
year = "2013",
doi = "10.1117/12.2009387",
language = "English",
isbn = "9780819493576",
volume = "8588",
booktitle = "Multiphoton Microscopy in the Biomedical Sciences XIII",

}

Ge, J, Wang, Y, Kuang, C, Lee, SS, Mazumder, N & Kao, FJ 2013, Fluorescence lifetime imaging with pulsed diode laser enabled stimulated emission. in Multiphoton Microscopy in the Biomedical Sciences XIII. vol. 8588, 858832, Multiphoton Microscopy in the Biomedical Sciences XIII, San Francisco, CA, United States, 03-02-13. https://doi.org/10.1117/12.2009387

Fluorescence lifetime imaging with pulsed diode laser enabled stimulated emission. / Ge, Jianhong; Wang, Yixin; Kuang, Cuifang; Lee, Shin Shian; Mazumder, Nirmal; Kao, Fu Jen.

Multiphoton Microscopy in the Biomedical Sciences XIII. Vol. 8588 2013. 858832.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

TY - GEN

T1 - Fluorescence lifetime imaging with pulsed diode laser enabled stimulated emission

AU - Ge, Jianhong

AU - Wang, Yixin

AU - Kuang, Cuifang

AU - Lee, Shin Shian

AU - Mazumder, Nirmal

AU - Kao, Fu Jen

PY - 2013

Y1 - 2013

N2 - We present here a stimulated emission based fluorescence lifetime imaging (FLIM) scheme using a pair of synchronized diode lasers operating at gain switched pulse mode. The two semiconductor lasers, with wavelengths at 635 nm and 700 nm, serve as the excitation and the stimulation light sources for the ATTO647N labeled sample, respectively. FLIM is readily achieved with their relative time delay controlled electronically. The coherent nature of the stimulated emission signal also allows FLIM at long working distance. In this way, a high performance all-semiconductor FLIM module is realized in a flexible, compact, and cost effective configuration.

AB - We present here a stimulated emission based fluorescence lifetime imaging (FLIM) scheme using a pair of synchronized diode lasers operating at gain switched pulse mode. The two semiconductor lasers, with wavelengths at 635 nm and 700 nm, serve as the excitation and the stimulation light sources for the ATTO647N labeled sample, respectively. FLIM is readily achieved with their relative time delay controlled electronically. The coherent nature of the stimulated emission signal also allows FLIM at long working distance. In this way, a high performance all-semiconductor FLIM module is realized in a flexible, compact, and cost effective configuration.

UR - http://www.scopus.com/inward/record.url?scp=84878706564&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84878706564&partnerID=8YFLogxK

U2 - 10.1117/12.2009387

DO - 10.1117/12.2009387

M3 - Conference contribution

SN - 9780819493576

VL - 8588

BT - Multiphoton Microscopy in the Biomedical Sciences XIII

ER -

Ge J, Wang Y, Kuang C, Lee SS, Mazumder N, Kao FJ. Fluorescence lifetime imaging with pulsed diode laser enabled stimulated emission. In Multiphoton Microscopy in the Biomedical Sciences XIII. Vol. 8588. 2013. 858832 https://doi.org/10.1117/12.2009387