Fluorescence lifetime imaging with pulsed diode laser enabled stimulated emission

Jianhong Ge, Yixin Wang, Cuifang Kuang, Shin Shian Lee, Nirmal Mazumder, Fu Jen Kao

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present here a stimulated emission based fluorescence lifetime imaging (FLIM) scheme using a pair of synchronized diode lasers operating at gain switched pulse mode. The two semiconductor lasers, with wavelengths at 635 nm and 700 nm, serve as the excitation and the stimulation light sources for the ATTO647N labeled sample, respectively. FLIM is readily achieved with their relative time delay controlled electronically. The coherent nature of the stimulated emission signal also allows FLIM at long working distance. In this way, a high performance all-semiconductor FLIM module is realized in a flexible, compact, and cost effective configuration.

Original languageEnglish
Title of host publicationMultiphoton Microscopy in the Biomedical Sciences XIII
Volume8588
DOIs
Publication statusPublished - 2013
EventMultiphoton Microscopy in the Biomedical Sciences XIII - San Francisco, CA, United States
Duration: 03-02-201305-02-2013

Conference

ConferenceMultiphoton Microscopy in the Biomedical Sciences XIII
CountryUnited States
CitySan Francisco, CA
Period03-02-1305-02-13

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All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Ge, J., Wang, Y., Kuang, C., Lee, S. S., Mazumder, N., & Kao, F. J. (2013). Fluorescence lifetime imaging with pulsed diode laser enabled stimulated emission. In Multiphoton Microscopy in the Biomedical Sciences XIII (Vol. 8588). [858832] https://doi.org/10.1117/12.2009387