Fluorimetric determination of rivastigmine in rat plasma by a reverse phase - High performance liquid chromatographic method: Application to a pharmacokinetic study

A. Karthik, G.S. Subramanian, M. Surulivelrajan, A. Ranjithkumar, S.B. Kamat

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A sensitive and selective high performance liquid chromatographic (HPLC) method was developed and validated for the rapid quantification of rivastigmine (CAS 123441-03-2) in micro quantity in rat plasma samples. The chromatographic separation was achieved with a reverse phase monomeric column C 18 (4.6 x 250 mm, 5 μm) and the mobile phase consisted of acetonitrile and 20 mmol/L phosphate buffer pH 3.0 (25:75) with a flow rate of 1 mL/min. The effluents were measured by fluorimetric detection with excitation and emission wavelengths at 220 nm and 293 nm, respectively. The calibration curve was linear (r 2 >0.99) ranging from 25-3000 ng/mL and the lower limit of quantification was 25 ng/mL. The method was validated with excellent sensitivity, selectivity, accuracy, precision, recovery and stability. The method has been successfully applied in a pharmacokinetic study of rivastigmine in rats. © ECV Editio Cantor Verlag.
Original languageEnglish
Pages (from-to)205-210
Number of pages6
JournalArzneimittel-Forschung/Drug Research
Issue number5
Publication statusPublished - 2008


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