Formulation and evaluation nasal insulin gel

T. Vamshi Krishna, Y. Madhusudan Rao

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The objective of the present study was to formulate insulin gel for intranasal administration and to evaluate it with respect to its invitro release studies and hypoglycemic activity in rabbits. The insulin gel was formulated using chitosan as gelling agent. The invivo efficacy of insulin gel administered intranasally was assessed by measuring the blood glucose levels at specified time intervals. The use of bioadhesive nasal gel containing insulin not only promoted the prolonged contact between the drug and the absorptive sites in the nasal cavity but also facilitated direct absorption of medicament through the nasal mucosa. Absorption of the drug through the nasal mucosa was high in the first 0.5 to 1.5 hours of the study with a sharp decline in blood glucose and the relative bioavailability of nasal insulin gel was found to be 19.48% of a marketed subcutaneous solution of insulin. This study further demonstrates that administration of insulin intranasally in gel form is a pleasant and painless alternative to injectable insulin.
Original languageEnglish
Pages (from-to)161-164
Number of pages4
JournalInternational Journal of Pharmacy and Pharmaceutical Sciences
Volume2
Issue number3
Publication statusPublished - 2010

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Nose
Gels
Insulin
Nasal Mucosa
Blood Glucose
Intranasal Administration
Nasal Cavity
Chitosan
Hypoglycemic Agents
Pharmaceutical Preparations
Biological Availability
Rabbits
Injections

Cite this

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title = "Formulation and evaluation nasal insulin gel",
abstract = "The objective of the present study was to formulate insulin gel for intranasal administration and to evaluate it with respect to its invitro release studies and hypoglycemic activity in rabbits. The insulin gel was formulated using chitosan as gelling agent. The invivo efficacy of insulin gel administered intranasally was assessed by measuring the blood glucose levels at specified time intervals. The use of bioadhesive nasal gel containing insulin not only promoted the prolonged contact between the drug and the absorptive sites in the nasal cavity but also facilitated direct absorption of medicament through the nasal mucosa. Absorption of the drug through the nasal mucosa was high in the first 0.5 to 1.5 hours of the study with a sharp decline in blood glucose and the relative bioavailability of nasal insulin gel was found to be 19.48{\%} of a marketed subcutaneous solution of insulin. This study further demonstrates that administration of insulin intranasally in gel form is a pleasant and painless alternative to injectable insulin.",
author = "{Vamshi Krishna}, T. and {Madhusudan Rao}, Y.",
note = "Cited By :6 Export Date: 10 November 2017 Correspondence Address: Vamshi Krishna, T.; Department of Pharmaceutics, Manipal College of Pharmaceutical Sciences, Manipal University, Udupi, Karnataka, India; email: krissrcm@gmail.com Chemicals/CAS: chitosan, 9012-76-4; glucose, 50-99-7, 84778-64-3; insulin, 9004-10-8; neutral insulin, 9004-14-2 Tradenames: actrapid Manufacturers: Torrent, India References: Varshosaz, J., Sadrai, H., Heidari, A., Nasal Delivery of Insulin using Bioadhesive Chitosan Gels (2006) Drug Delivery, 13, pp. 31-38; Souza-Reshma, D., Mutalik, S., Vidyasagar, S., Venkatesh, M., Udupa, N., Nasal insulin gel as an alternate to parenteral insulin (2005) AAPS PharmSciTech, 6 (2), p. 27; Najefabadi, A.H., Moslemi, P., Tajerzadeh, H., (2004) Intranasal Bioavailability of Insulin From Carbopol Based Gel Spray In Rabbit;Drug Delivery, 11, pp. 295-300; Chein, Y.W., Su, K.S.E., Chang, S.F., Nasal systemic drug delivery (1989) Dekker, pp. 1-77; William, T., Cefalu, M.D., Concept, strategies and feasibility of non invasive insulin delivery (2004) Diabetes Care, 27, pp. 239-246; Thanou, M., Verhoef, J.C., Junginger, H.E., Oral drug absorption enhancement by chitosan and its derivatives, Adv (2001) Drug Delivery, 52, pp. 117-126; Heinemann, L., Pfutzner, A., Heise, T., Alternative routes of administration as an approach to improve insulin therapy: Update on dermal, oral, nasal and pulmonary insulin delivery (2001) Curr Pharm Res, 7, pp. 1327-1351; Dodane, V., Amin-Khan, M., Merwin, J.R., Effect of chitosan on epithelial permeability and structure (1999) Int. J. Pharm. 182 21-, p. 32; Beht, Optimization of systemic nasal drug delivery with pharmaceutical excipients (1998) Adv. Drug. Del. Rev, 29, pp. 117-133; Kadam, S.S., Mahadik, K.R., Pawar, A.P., Paradkar, A.R., Transnasal delivery of peptides - a review (1993) The East Pharm, pp. 47-49; Lee, W.A., Narog, B.A., Patapoff, T.W., Wang, Y.J., Intranasal bioavailability of insulin powder formulation: Effect of permeation enhancer to protein ratio (1991) J. Pharm. Sci. 80,, pp. 725-729; Schipper, The nasal mucociliary clearance: Relevance to nasal drug delivery{"} (1991) Pharma. Res, 7, pp. 807-814",
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Formulation and evaluation nasal insulin gel. / Vamshi Krishna, T.; Madhusudan Rao, Y.

In: International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 2, No. 3, 2010, p. 161-164.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Formulation and evaluation nasal insulin gel

AU - Vamshi Krishna, T.

AU - Madhusudan Rao, Y.

N1 - Cited By :6 Export Date: 10 November 2017 Correspondence Address: Vamshi Krishna, T.; Department of Pharmaceutics, Manipal College of Pharmaceutical Sciences, Manipal University, Udupi, Karnataka, India; email: krissrcm@gmail.com Chemicals/CAS: chitosan, 9012-76-4; glucose, 50-99-7, 84778-64-3; insulin, 9004-10-8; neutral insulin, 9004-14-2 Tradenames: actrapid Manufacturers: Torrent, India References: Varshosaz, J., Sadrai, H., Heidari, A., Nasal Delivery of Insulin using Bioadhesive Chitosan Gels (2006) Drug Delivery, 13, pp. 31-38; Souza-Reshma, D., Mutalik, S., Vidyasagar, S., Venkatesh, M., Udupa, N., Nasal insulin gel as an alternate to parenteral insulin (2005) AAPS PharmSciTech, 6 (2), p. 27; Najefabadi, A.H., Moslemi, P., Tajerzadeh, H., (2004) Intranasal Bioavailability of Insulin From Carbopol Based Gel Spray In Rabbit;Drug Delivery, 11, pp. 295-300; Chein, Y.W., Su, K.S.E., Chang, S.F., Nasal systemic drug delivery (1989) Dekker, pp. 1-77; William, T., Cefalu, M.D., Concept, strategies and feasibility of non invasive insulin delivery (2004) Diabetes Care, 27, pp. 239-246; Thanou, M., Verhoef, J.C., Junginger, H.E., Oral drug absorption enhancement by chitosan and its derivatives, Adv (2001) Drug Delivery, 52, pp. 117-126; Heinemann, L., Pfutzner, A., Heise, T., Alternative routes of administration as an approach to improve insulin therapy: Update on dermal, oral, nasal and pulmonary insulin delivery (2001) Curr Pharm Res, 7, pp. 1327-1351; Dodane, V., Amin-Khan, M., Merwin, J.R., Effect of chitosan on epithelial permeability and structure (1999) Int. J. Pharm. 182 21-, p. 32; Beht, Optimization of systemic nasal drug delivery with pharmaceutical excipients (1998) Adv. Drug. Del. Rev, 29, pp. 117-133; Kadam, S.S., Mahadik, K.R., Pawar, A.P., Paradkar, A.R., Transnasal delivery of peptides - a review (1993) The East Pharm, pp. 47-49; Lee, W.A., Narog, B.A., Patapoff, T.W., Wang, Y.J., Intranasal bioavailability of insulin powder formulation: Effect of permeation enhancer to protein ratio (1991) J. Pharm. Sci. 80,, pp. 725-729; Schipper, The nasal mucociliary clearance: Relevance to nasal drug delivery" (1991) Pharma. Res, 7, pp. 807-814

PY - 2010

Y1 - 2010

N2 - The objective of the present study was to formulate insulin gel for intranasal administration and to evaluate it with respect to its invitro release studies and hypoglycemic activity in rabbits. The insulin gel was formulated using chitosan as gelling agent. The invivo efficacy of insulin gel administered intranasally was assessed by measuring the blood glucose levels at specified time intervals. The use of bioadhesive nasal gel containing insulin not only promoted the prolonged contact between the drug and the absorptive sites in the nasal cavity but also facilitated direct absorption of medicament through the nasal mucosa. Absorption of the drug through the nasal mucosa was high in the first 0.5 to 1.5 hours of the study with a sharp decline in blood glucose and the relative bioavailability of nasal insulin gel was found to be 19.48% of a marketed subcutaneous solution of insulin. This study further demonstrates that administration of insulin intranasally in gel form is a pleasant and painless alternative to injectable insulin.

AB - The objective of the present study was to formulate insulin gel for intranasal administration and to evaluate it with respect to its invitro release studies and hypoglycemic activity in rabbits. The insulin gel was formulated using chitosan as gelling agent. The invivo efficacy of insulin gel administered intranasally was assessed by measuring the blood glucose levels at specified time intervals. The use of bioadhesive nasal gel containing insulin not only promoted the prolonged contact between the drug and the absorptive sites in the nasal cavity but also facilitated direct absorption of medicament through the nasal mucosa. Absorption of the drug through the nasal mucosa was high in the first 0.5 to 1.5 hours of the study with a sharp decline in blood glucose and the relative bioavailability of nasal insulin gel was found to be 19.48% of a marketed subcutaneous solution of insulin. This study further demonstrates that administration of insulin intranasally in gel form is a pleasant and painless alternative to injectable insulin.

M3 - Article

VL - 2

SP - 161

EP - 164

JO - International Journal of Pharmacy and Pharmaceutical Sciences

JF - International Journal of Pharmacy and Pharmaceutical Sciences

SN - 0975-1491

IS - 3

ER -