Formulation of thermoreversible gel of cranberry juice concentrate

Evaluation, biocompatibility studies and its antimicrobial activity against periodontal pathogens

H. R. Rajeshwari, Dinesh Dhamecha, Satveer Jagwani, Dhanashree Patil, Satisha Hegde, Raksha Potdar, Renuka Metgud, Sunil Jalalpure, Subarna Roy, Kiran Jadhav, Neeraj Kumar Tiwari, Sravani Koduru, Shweta Hugar, Suneel Dodamani

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The present work aims to investigate the efficacy of thermoreversible gel of cranberry juice concentrate (CJC) as local drug delivery for the treatment of periodontitis. CJC was initially tested for its antimicrobial activities like MIC, MBC, antiadhesion, antibiofilm and time kill assay against the panel of organisms (S. mutans (SM), E. faecalis (EF), A. actinomycetemcomitans (AA), P. gingivalis (PG), T. forsythia (TF)) responsible for periapical and periodontal infections. Antimicrobial activity of CJC showed MIC value of 50 mg/ml and MBC value of 100 mg/ml with desirable antiadhesion (83–90%) and antibiofilm activity (70–85%). CJC was evaluated for its biocompatibility using periodontal fibroblasts by cell based MTT assay and found to be nontoxic. Influence of CJC on periodontopathogen PG derived virulence factors (fimA and kgp) was studied using real time polymerase chain reaction (RT-PCR) technique wherein down regulation of selected genes demonstrated inhibitory effect against PG virulence factors. Thermoreversible gel of CJC was formulated by cold method using poloxamer 407 as thermosensitive polymer and carbopol 934 as mucoadhesive polymer and evaluated for its gelation temperature, viscosity, gel strength and mucoadhesive strength. Comparison of optimized thermoreversible gel of CJC (500 mg/ml) with commercially available chlorhexidine gluconate gel (0.2%) using agar well diffusion demonstrated equal zone of inhibition against SM, EF, AA, PG & TF. Hence the formulated thermoreversible gel of CJC could serve as a novel herbal alternative to currently available periodontal treatment modalities.

Original languageEnglish
Pages (from-to)1506-1514
Number of pages9
JournalMaterials Science and Engineering C
Volume75
DOIs
Publication statusPublished - 01-06-2017

Fingerprint

juices
pathogens
biocompatibility
Pathogens
Biocompatibility
Gels
gels
formulations
evaluation
virulence
Virulence Factors
Assays
Polymers
Poloxamer
Polymerase chain reaction
Gelation
Fibroblasts
Drug delivery
polymerase chain reaction
Agar

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Rajeshwari, H. R. ; Dhamecha, Dinesh ; Jagwani, Satveer ; Patil, Dhanashree ; Hegde, Satisha ; Potdar, Raksha ; Metgud, Renuka ; Jalalpure, Sunil ; Roy, Subarna ; Jadhav, Kiran ; Tiwari, Neeraj Kumar ; Koduru, Sravani ; Hugar, Shweta ; Dodamani, Suneel. / Formulation of thermoreversible gel of cranberry juice concentrate : Evaluation, biocompatibility studies and its antimicrobial activity against periodontal pathogens. In: Materials Science and Engineering C. 2017 ; Vol. 75. pp. 1506-1514.
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abstract = "The present work aims to investigate the efficacy of thermoreversible gel of cranberry juice concentrate (CJC) as local drug delivery for the treatment of periodontitis. CJC was initially tested for its antimicrobial activities like MIC, MBC, antiadhesion, antibiofilm and time kill assay against the panel of organisms (S. mutans (SM), E. faecalis (EF), A. actinomycetemcomitans (AA), P. gingivalis (PG), T. forsythia (TF)) responsible for periapical and periodontal infections. Antimicrobial activity of CJC showed MIC value of 50 mg/ml and MBC value of 100 mg/ml with desirable antiadhesion (83–90{\%}) and antibiofilm activity (70–85{\%}). CJC was evaluated for its biocompatibility using periodontal fibroblasts by cell based MTT assay and found to be nontoxic. Influence of CJC on periodontopathogen PG derived virulence factors (fimA and kgp) was studied using real time polymerase chain reaction (RT-PCR) technique wherein down regulation of selected genes demonstrated inhibitory effect against PG virulence factors. Thermoreversible gel of CJC was formulated by cold method using poloxamer 407 as thermosensitive polymer and carbopol 934 as mucoadhesive polymer and evaluated for its gelation temperature, viscosity, gel strength and mucoadhesive strength. Comparison of optimized thermoreversible gel of CJC (500 mg/ml) with commercially available chlorhexidine gluconate gel (0.2{\%}) using agar well diffusion demonstrated equal zone of inhibition against SM, EF, AA, PG & TF. Hence the formulated thermoreversible gel of CJC could serve as a novel herbal alternative to currently available periodontal treatment modalities.",
author = "Rajeshwari, {H. R.} and Dinesh Dhamecha and Satveer Jagwani and Dhanashree Patil and Satisha Hegde and Raksha Potdar and Renuka Metgud and Sunil Jalalpure and Subarna Roy and Kiran Jadhav and Tiwari, {Neeraj Kumar} and Sravani Koduru and Shweta Hugar and Suneel Dodamani",
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Rajeshwari, HR, Dhamecha, D, Jagwani, S, Patil, D, Hegde, S, Potdar, R, Metgud, R, Jalalpure, S, Roy, S, Jadhav, K, Tiwari, NK, Koduru, S, Hugar, S & Dodamani, S 2017, 'Formulation of thermoreversible gel of cranberry juice concentrate: Evaluation, biocompatibility studies and its antimicrobial activity against periodontal pathogens', Materials Science and Engineering C, vol. 75, pp. 1506-1514. https://doi.org/10.1016/j.msec.2017.03.054

Formulation of thermoreversible gel of cranberry juice concentrate : Evaluation, biocompatibility studies and its antimicrobial activity against periodontal pathogens. / Rajeshwari, H. R.; Dhamecha, Dinesh; Jagwani, Satveer; Patil, Dhanashree; Hegde, Satisha; Potdar, Raksha; Metgud, Renuka; Jalalpure, Sunil; Roy, Subarna; Jadhav, Kiran; Tiwari, Neeraj Kumar; Koduru, Sravani; Hugar, Shweta; Dodamani, Suneel.

In: Materials Science and Engineering C, Vol. 75, 01.06.2017, p. 1506-1514.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Formulation of thermoreversible gel of cranberry juice concentrate

T2 - Evaluation, biocompatibility studies and its antimicrobial activity against periodontal pathogens

AU - Rajeshwari, H. R.

AU - Dhamecha, Dinesh

AU - Jagwani, Satveer

AU - Patil, Dhanashree

AU - Hegde, Satisha

AU - Potdar, Raksha

AU - Metgud, Renuka

AU - Jalalpure, Sunil

AU - Roy, Subarna

AU - Jadhav, Kiran

AU - Tiwari, Neeraj Kumar

AU - Koduru, Sravani

AU - Hugar, Shweta

AU - Dodamani, Suneel

PY - 2017/6/1

Y1 - 2017/6/1

N2 - The present work aims to investigate the efficacy of thermoreversible gel of cranberry juice concentrate (CJC) as local drug delivery for the treatment of periodontitis. CJC was initially tested for its antimicrobial activities like MIC, MBC, antiadhesion, antibiofilm and time kill assay against the panel of organisms (S. mutans (SM), E. faecalis (EF), A. actinomycetemcomitans (AA), P. gingivalis (PG), T. forsythia (TF)) responsible for periapical and periodontal infections. Antimicrobial activity of CJC showed MIC value of 50 mg/ml and MBC value of 100 mg/ml with desirable antiadhesion (83–90%) and antibiofilm activity (70–85%). CJC was evaluated for its biocompatibility using periodontal fibroblasts by cell based MTT assay and found to be nontoxic. Influence of CJC on periodontopathogen PG derived virulence factors (fimA and kgp) was studied using real time polymerase chain reaction (RT-PCR) technique wherein down regulation of selected genes demonstrated inhibitory effect against PG virulence factors. Thermoreversible gel of CJC was formulated by cold method using poloxamer 407 as thermosensitive polymer and carbopol 934 as mucoadhesive polymer and evaluated for its gelation temperature, viscosity, gel strength and mucoadhesive strength. Comparison of optimized thermoreversible gel of CJC (500 mg/ml) with commercially available chlorhexidine gluconate gel (0.2%) using agar well diffusion demonstrated equal zone of inhibition against SM, EF, AA, PG & TF. Hence the formulated thermoreversible gel of CJC could serve as a novel herbal alternative to currently available periodontal treatment modalities.

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