Fourier transform infrared spectroscopic studies on modulation of n-nitrosodiethylamine-induced hepatocarcinogenesis by azadirachta indica

Sanjay Bharati, Praveen Rishi, Ashwani Koul

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Fourier transform infrared spectroscopy was employed in the present study to obtain information about the molecular composition of hepatic tumor versus hepatic tissue. A hepatic cancer model was developed by administering N-nitrosodiethylamine (NDEA) to male Balb/c mice. The results revealed that NDEA-induced hepatic cancer tumor tissue had altered molecular composition compared with normal liver tissue. Compared with the normal tissue, the saturation level of membrane phospholipids was observed to be decreased in tumors along with an abnormal distribution of protein secondary structures. A signifcant decrease in glycogen and a signifcant increase in total nucleic acid content were also observed in tumor cells. The administration of aqueous Azadirachta indica leaf extract (AAILE) prior to NDEA treatment resulted in the normalization of saturation levels in phospholipids and total nucleic acid content and in the distribution of protein secondary structures in tumors. A signifcant increase in the amount of stored glycogen was observed in AAILE cotreated tumors compared with NDEA-induced tumors, which might indicate that AAILE cotreatment impeded the ability of tumor cells to consume glucose at a faster rate. The normalization of molecular composition upon AAILE cotreatment in hepatic tumors might indicate that AAILE hampered the process of evolution of tumors, which could be responsible for its observed chemopreventive action.

Original languageEnglish
Pages (from-to)227-236
Number of pages10
JournalJournal of Environmental Pathology, Toxicology and Oncology
Volume34
Issue number3
DOIs
Publication statusPublished - 29-06-2015

Fingerprint

Azadirachta
Diethylnitrosamine
Fourier Analysis
Tumors
Fourier transforms
Modulation
Infrared radiation
Neoplasms
Tissue
Secondary Protein Structure
Liver
Liver Neoplasms
Glycogen
Nucleic Acids
Phospholipids
Chemical analysis
Cells
Fourier Transform Infrared Spectroscopy
Proteins

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

@article{b6630bd4f2994f278471ab4acd7a8c94,
title = "Fourier transform infrared spectroscopic studies on modulation of n-nitrosodiethylamine-induced hepatocarcinogenesis by azadirachta indica",
abstract = "Fourier transform infrared spectroscopy was employed in the present study to obtain information about the molecular composition of hepatic tumor versus hepatic tissue. A hepatic cancer model was developed by administering N-nitrosodiethylamine (NDEA) to male Balb/c mice. The results revealed that NDEA-induced hepatic cancer tumor tissue had altered molecular composition compared with normal liver tissue. Compared with the normal tissue, the saturation level of membrane phospholipids was observed to be decreased in tumors along with an abnormal distribution of protein secondary structures. A signifcant decrease in glycogen and a signifcant increase in total nucleic acid content were also observed in tumor cells. The administration of aqueous Azadirachta indica leaf extract (AAILE) prior to NDEA treatment resulted in the normalization of saturation levels in phospholipids and total nucleic acid content and in the distribution of protein secondary structures in tumors. A signifcant increase in the amount of stored glycogen was observed in AAILE cotreated tumors compared with NDEA-induced tumors, which might indicate that AAILE cotreatment impeded the ability of tumor cells to consume glucose at a faster rate. The normalization of molecular composition upon AAILE cotreatment in hepatic tumors might indicate that AAILE hampered the process of evolution of tumors, which could be responsible for its observed chemopreventive action.",
author = "Sanjay Bharati and Praveen Rishi and Ashwani Koul",
year = "2015",
month = "6",
day = "29",
doi = "10.1615/JEnvironPatholToxicolOncol.2015012145",
language = "English",
volume = "34",
pages = "227--236",
journal = "Journal of Environmental Pathology, Toxicology and Oncology",
issn = "0731-8898",
publisher = "Begell House Inc.",
number = "3",

}

TY - JOUR

T1 - Fourier transform infrared spectroscopic studies on modulation of n-nitrosodiethylamine-induced hepatocarcinogenesis by azadirachta indica

AU - Bharati, Sanjay

AU - Rishi, Praveen

AU - Koul, Ashwani

PY - 2015/6/29

Y1 - 2015/6/29

N2 - Fourier transform infrared spectroscopy was employed in the present study to obtain information about the molecular composition of hepatic tumor versus hepatic tissue. A hepatic cancer model was developed by administering N-nitrosodiethylamine (NDEA) to male Balb/c mice. The results revealed that NDEA-induced hepatic cancer tumor tissue had altered molecular composition compared with normal liver tissue. Compared with the normal tissue, the saturation level of membrane phospholipids was observed to be decreased in tumors along with an abnormal distribution of protein secondary structures. A signifcant decrease in glycogen and a signifcant increase in total nucleic acid content were also observed in tumor cells. The administration of aqueous Azadirachta indica leaf extract (AAILE) prior to NDEA treatment resulted in the normalization of saturation levels in phospholipids and total nucleic acid content and in the distribution of protein secondary structures in tumors. A signifcant increase in the amount of stored glycogen was observed in AAILE cotreated tumors compared with NDEA-induced tumors, which might indicate that AAILE cotreatment impeded the ability of tumor cells to consume glucose at a faster rate. The normalization of molecular composition upon AAILE cotreatment in hepatic tumors might indicate that AAILE hampered the process of evolution of tumors, which could be responsible for its observed chemopreventive action.

AB - Fourier transform infrared spectroscopy was employed in the present study to obtain information about the molecular composition of hepatic tumor versus hepatic tissue. A hepatic cancer model was developed by administering N-nitrosodiethylamine (NDEA) to male Balb/c mice. The results revealed that NDEA-induced hepatic cancer tumor tissue had altered molecular composition compared with normal liver tissue. Compared with the normal tissue, the saturation level of membrane phospholipids was observed to be decreased in tumors along with an abnormal distribution of protein secondary structures. A signifcant decrease in glycogen and a signifcant increase in total nucleic acid content were also observed in tumor cells. The administration of aqueous Azadirachta indica leaf extract (AAILE) prior to NDEA treatment resulted in the normalization of saturation levels in phospholipids and total nucleic acid content and in the distribution of protein secondary structures in tumors. A signifcant increase in the amount of stored glycogen was observed in AAILE cotreated tumors compared with NDEA-induced tumors, which might indicate that AAILE cotreatment impeded the ability of tumor cells to consume glucose at a faster rate. The normalization of molecular composition upon AAILE cotreatment in hepatic tumors might indicate that AAILE hampered the process of evolution of tumors, which could be responsible for its observed chemopreventive action.

UR - http://www.scopus.com/inward/record.url?scp=84933531606&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84933531606&partnerID=8YFLogxK

U2 - 10.1615/JEnvironPatholToxicolOncol.2015012145

DO - 10.1615/JEnvironPatholToxicolOncol.2015012145

M3 - Article

C2 - 26349605

AN - SCOPUS:84933531606

VL - 34

SP - 227

EP - 236

JO - Journal of Environmental Pathology, Toxicology and Oncology

JF - Journal of Environmental Pathology, Toxicology and Oncology

SN - 0731-8898

IS - 3

ER -