Free radical scavenging activity of aqueous root extract of Argyreia nervosa (Burm.f.) Boj. (Convolvulaceae)

C.S. Shreedhara, H.N. Aswatha Ram, S.B. Zanwar, G.P. Falguni

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Aqueous extract of Argyreia nervosa, which is widely used in the indigenous system of medicine, was studied for its in vitro scavenging activity by different methods viz. DPPH radical scavenging, ABTS radical scavenging, lipid peroxidation, iron chelating activity, superoxide scavenging, total antioxidant capacity, Nitric oxide scavenging and Non-enzymatic Glycosylation of Haemoglobin assay. The results were analyzed statistically by regression method. Its antioxidant activity was estimated by IC50 value and the values are 88.86 μg/ml for DPPH radical scavenging, 66.64 μg/ml for ABTS radical scavenging, 31.57 μg/ml for lipid peroxidation, 8.0 μg/ml for iron chelating activity, 113.20 μg/ml for Nitric oxide scavenging and 7.51 μg/ml for superoxide scavenging. Total antioxidant capacity was found to be 22.0 μg/ml. In Haemoglobin glycosylation the % scavenging was found to be 43.77% and 64.42% for concentration 0.5 mg/ml. and 1.0 mg/ml respectively. In all the methods, the extract showed its ability to scavenge free radicals in a concentration dependent manner. The results indicate that Argyreia nervosa has significant antioxidant activity.
Original languageEnglish
Pages (from-to)216-223
Number of pages8
JournalJournal of Natural Remedies
Volume9
Issue number2
Publication statusPublished - 2009

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Convolvulaceae
Free Radicals
Antioxidants
Glycosylation
Superoxides
Lipid Peroxidation
Nitric Oxide
Hemoglobins
Iron
Traditional Medicine
Systems Analysis
Inhibitory Concentration 50

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@article{48c5cdd6dcd74b2da4fc3031002f7e30,
title = "Free radical scavenging activity of aqueous root extract of Argyreia nervosa (Burm.f.) Boj. (Convolvulaceae)",
abstract = "Aqueous extract of Argyreia nervosa, which is widely used in the indigenous system of medicine, was studied for its in vitro scavenging activity by different methods viz. DPPH radical scavenging, ABTS radical scavenging, lipid peroxidation, iron chelating activity, superoxide scavenging, total antioxidant capacity, Nitric oxide scavenging and Non-enzymatic Glycosylation of Haemoglobin assay. The results were analyzed statistically by regression method. Its antioxidant activity was estimated by IC50 value and the values are 88.86 μg/ml for DPPH radical scavenging, 66.64 μg/ml for ABTS radical scavenging, 31.57 μg/ml for lipid peroxidation, 8.0 μg/ml for iron chelating activity, 113.20 μg/ml for Nitric oxide scavenging and 7.51 μg/ml for superoxide scavenging. Total antioxidant capacity was found to be 22.0 μg/ml. In Haemoglobin glycosylation the {\%} scavenging was found to be 43.77{\%} and 64.42{\%} for concentration 0.5 mg/ml. and 1.0 mg/ml respectively. In all the methods, the extract showed its ability to scavenge free radicals in a concentration dependent manner. The results indicate that Argyreia nervosa has significant antioxidant activity.",
author = "C.S. Shreedhara and {Aswatha Ram}, H.N. and S.B. Zanwar and G.P. Falguni",
note = "Cited By :3 Export Date: 10 November 2017 CODEN: JNROA Correspondence Address: Shreedhara, C. S.; Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal - 576 104, India; email: cssncp@rediffmail.com Chemicals/CAS: 1,1 diphenyl 2 picrylhydrazyl, 1898-66-4; 2,2' azinobis(3 ethylbenzothiazoline 6 sulfonic acid), 28752-68-3; alpha tocopherol, 1406-18-4, 1406-70-8, 52225-20-4, 58-95-7, 59-02-9; ascorbic acid, 134-03-2, 15421-15-5, 50-81-7; hemoglobin, 9008-02-0; nitric oxide, 10102-43-9; superoxide, 11062-77-4 References: Shirwaikar, A., Shirwaikar, A., Punitha, I.S.R., (2007) Natural Product Sciences., 13 (1), pp. 40-45; Longman, O., (2003) Indian Medicinal Plants, a Compendium of 500 Species, 4, p. 264; Molyneux, P., (2004) Sci. and Technol., 26 (2), pp. 212-219; Schlesier, K., Harwart, M., Bohm, V., Bitsch, R., (2002) Free Radic.Res., 30 (2), pp. 177-185; Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., Rice Evans, C., (1998) Free Radic. Biol. Med., 72, pp. 1231-1237; Blois, (1958) Nature, 26, pp. 1199-1201; Benzie, I.F.F., Strain, J.T., (1996) Anal. Biochem., 239, pp. 70-76; Benzie, I.F.F., Szeto, Y.T., (1999) J. Agri. Food Chem., 47, pp. 633-636; Govindrarajan, R., Vijaykumar, M., Rawat, A.K.S., Mehrotra, S., (2003) Indian J. Exp. Biol., 41, p. 875; Muthunjaya, K., Hukkeri, V.I., (2008) PHCOG MAG., 4 (13), pp. 42-51; Sreejayan, N., Rao, M.N.A., (1997) J. Pharm. Pharmacol., 49, pp. 105-107. , a; Sreejayan, N., Rao, M.N.A., (1996) Drug Res., 46, pp. 169-172. , b; Shirwaikar, A., Rajendra, K., Dinesh Kumar, C., (2004) Indian J. Exp. Biol., 42, pp. 803-807; Preito, P., Pinedo, M., Aguilar, M., (1999) Anal. Biochem., 269, pp. 337-341; Pal, D.K., Dutta, S., (2006) Indian Journal of Pharmaceutical Sciences., 68 (2), pp. 256-258; Marx, J.L., (1987) Science., 235, pp. 512-529; Arora, A., Sairam, R.K., Srivastava, G.C., (2002) Cur. Sci., 82, pp. 1227-1229; Geesin, J.G., Gordon, J.S., Berg, R.A., (1990) Arch. Biochem. Biphy., 278, pp. 352-355; Badmi, S., Gupta, M.K., Suresh, B., (2003) J. Ethanopharmacol., 85, pp. 227-230; Sanchez-Morino, C., (2002) Food Sci. and Technol. Int., 8, pp. 122-126; Mahakunakorn, P., Tohda, M., Murakami, Y., Matsumoto, K., Watanabe, H., (2004) Biol.Pharm. Bull., 27 (1), pp. 38-46; Chang, L.W., Yen, W.J., Huang, S.C., Duh, P.D., (2002) Food Chem., 78, pp. 347-354; Halliwell, B., (1991) Am. J. Med., 91, pp. 14S-22S; Duh, P.D., Tu, Y.Y., Yen, G.C., (1999) Lebensmittel-Wissenschaft and Technologie, 32, pp. 269-277; Gordon, M.H., The mechanism of the antioxidant action in vitro (1990) Food Antioxidants, pp. 1-18. , Hudson, BJF (Ed.), Ekseveir, London; Rice-Evans, C., Miller, N.J., (1997) Free Radic. Res., 195, pp. 26-27; Hussain, S.A., Ray, G., (2002) Indian J. Exp. Biol., 40, pp. 1213-1232; Ialenti, A., Moncada, S., Di Rosa, M., (1993) British J. Pharmacol., 362, pp. 801-805; Cross, A.R., Jones, O.T.G., (1991) Biophys. Acta., 387, pp. 281-285; Wiseman, H., Halliwell, B., (1996) Biochem. J., 313, pp. 17-19; Shirwaikar, A., Prabhu, K.S., Punitha, I.S.R., (2006) Indian J. Exp. Biol., 44, pp. 993-996",
year = "2009",
language = "English",
volume = "9",
pages = "216--223",
journal = "Journal of Natural Remedies",
issn = "0972-5547",
publisher = "Natural Remedies Private Limited",
number = "2",

}

Free radical scavenging activity of aqueous root extract of Argyreia nervosa (Burm.f.) Boj. (Convolvulaceae). / Shreedhara, C.S.; Aswatha Ram, H.N.; Zanwar, S.B.; Falguni, G.P.

In: Journal of Natural Remedies, Vol. 9, No. 2, 2009, p. 216-223.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Free radical scavenging activity of aqueous root extract of Argyreia nervosa (Burm.f.) Boj. (Convolvulaceae)

AU - Shreedhara, C.S.

AU - Aswatha Ram, H.N.

AU - Zanwar, S.B.

AU - Falguni, G.P.

N1 - Cited By :3 Export Date: 10 November 2017 CODEN: JNROA Correspondence Address: Shreedhara, C. S.; Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal - 576 104, India; email: cssncp@rediffmail.com Chemicals/CAS: 1,1 diphenyl 2 picrylhydrazyl, 1898-66-4; 2,2' azinobis(3 ethylbenzothiazoline 6 sulfonic acid), 28752-68-3; alpha tocopherol, 1406-18-4, 1406-70-8, 52225-20-4, 58-95-7, 59-02-9; ascorbic acid, 134-03-2, 15421-15-5, 50-81-7; hemoglobin, 9008-02-0; nitric oxide, 10102-43-9; superoxide, 11062-77-4 References: Shirwaikar, A., Shirwaikar, A., Punitha, I.S.R., (2007) Natural Product Sciences., 13 (1), pp. 40-45; Longman, O., (2003) Indian Medicinal Plants, a Compendium of 500 Species, 4, p. 264; Molyneux, P., (2004) Sci. and Technol., 26 (2), pp. 212-219; Schlesier, K., Harwart, M., Bohm, V., Bitsch, R., (2002) Free Radic.Res., 30 (2), pp. 177-185; Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., Rice Evans, C., (1998) Free Radic. Biol. Med., 72, pp. 1231-1237; Blois, (1958) Nature, 26, pp. 1199-1201; Benzie, I.F.F., Strain, J.T., (1996) Anal. Biochem., 239, pp. 70-76; Benzie, I.F.F., Szeto, Y.T., (1999) J. Agri. Food Chem., 47, pp. 633-636; Govindrarajan, R., Vijaykumar, M., Rawat, A.K.S., Mehrotra, S., (2003) Indian J. Exp. Biol., 41, p. 875; Muthunjaya, K., Hukkeri, V.I., (2008) PHCOG MAG., 4 (13), pp. 42-51; Sreejayan, N., Rao, M.N.A., (1997) J. Pharm. Pharmacol., 49, pp. 105-107. , a; Sreejayan, N., Rao, M.N.A., (1996) Drug Res., 46, pp. 169-172. , b; Shirwaikar, A., Rajendra, K., Dinesh Kumar, C., (2004) Indian J. Exp. Biol., 42, pp. 803-807; Preito, P., Pinedo, M., Aguilar, M., (1999) Anal. Biochem., 269, pp. 337-341; Pal, D.K., Dutta, S., (2006) Indian Journal of Pharmaceutical Sciences., 68 (2), pp. 256-258; Marx, J.L., (1987) Science., 235, pp. 512-529; Arora, A., Sairam, R.K., Srivastava, G.C., (2002) Cur. Sci., 82, pp. 1227-1229; Geesin, J.G., Gordon, J.S., Berg, R.A., (1990) Arch. Biochem. Biphy., 278, pp. 352-355; Badmi, S., Gupta, M.K., Suresh, B., (2003) J. Ethanopharmacol., 85, pp. 227-230; Sanchez-Morino, C., (2002) Food Sci. and Technol. Int., 8, pp. 122-126; Mahakunakorn, P., Tohda, M., Murakami, Y., Matsumoto, K., Watanabe, H., (2004) Biol.Pharm. Bull., 27 (1), pp. 38-46; Chang, L.W., Yen, W.J., Huang, S.C., Duh, P.D., (2002) Food Chem., 78, pp. 347-354; Halliwell, B., (1991) Am. J. Med., 91, pp. 14S-22S; Duh, P.D., Tu, Y.Y., Yen, G.C., (1999) Lebensmittel-Wissenschaft and Technologie, 32, pp. 269-277; Gordon, M.H., The mechanism of the antioxidant action in vitro (1990) Food Antioxidants, pp. 1-18. , Hudson, BJF (Ed.), Ekseveir, London; Rice-Evans, C., Miller, N.J., (1997) Free Radic. Res., 195, pp. 26-27; Hussain, S.A., Ray, G., (2002) Indian J. Exp. Biol., 40, pp. 1213-1232; Ialenti, A., Moncada, S., Di Rosa, M., (1993) British J. Pharmacol., 362, pp. 801-805; Cross, A.R., Jones, O.T.G., (1991) Biophys. Acta., 387, pp. 281-285; Wiseman, H., Halliwell, B., (1996) Biochem. J., 313, pp. 17-19; Shirwaikar, A., Prabhu, K.S., Punitha, I.S.R., (2006) Indian J. Exp. Biol., 44, pp. 993-996

PY - 2009

Y1 - 2009

N2 - Aqueous extract of Argyreia nervosa, which is widely used in the indigenous system of medicine, was studied for its in vitro scavenging activity by different methods viz. DPPH radical scavenging, ABTS radical scavenging, lipid peroxidation, iron chelating activity, superoxide scavenging, total antioxidant capacity, Nitric oxide scavenging and Non-enzymatic Glycosylation of Haemoglobin assay. The results were analyzed statistically by regression method. Its antioxidant activity was estimated by IC50 value and the values are 88.86 μg/ml for DPPH radical scavenging, 66.64 μg/ml for ABTS radical scavenging, 31.57 μg/ml for lipid peroxidation, 8.0 μg/ml for iron chelating activity, 113.20 μg/ml for Nitric oxide scavenging and 7.51 μg/ml for superoxide scavenging. Total antioxidant capacity was found to be 22.0 μg/ml. In Haemoglobin glycosylation the % scavenging was found to be 43.77% and 64.42% for concentration 0.5 mg/ml. and 1.0 mg/ml respectively. In all the methods, the extract showed its ability to scavenge free radicals in a concentration dependent manner. The results indicate that Argyreia nervosa has significant antioxidant activity.

AB - Aqueous extract of Argyreia nervosa, which is widely used in the indigenous system of medicine, was studied for its in vitro scavenging activity by different methods viz. DPPH radical scavenging, ABTS radical scavenging, lipid peroxidation, iron chelating activity, superoxide scavenging, total antioxidant capacity, Nitric oxide scavenging and Non-enzymatic Glycosylation of Haemoglobin assay. The results were analyzed statistically by regression method. Its antioxidant activity was estimated by IC50 value and the values are 88.86 μg/ml for DPPH radical scavenging, 66.64 μg/ml for ABTS radical scavenging, 31.57 μg/ml for lipid peroxidation, 8.0 μg/ml for iron chelating activity, 113.20 μg/ml for Nitric oxide scavenging and 7.51 μg/ml for superoxide scavenging. Total antioxidant capacity was found to be 22.0 μg/ml. In Haemoglobin glycosylation the % scavenging was found to be 43.77% and 64.42% for concentration 0.5 mg/ml. and 1.0 mg/ml respectively. In all the methods, the extract showed its ability to scavenge free radicals in a concentration dependent manner. The results indicate that Argyreia nervosa has significant antioxidant activity.

M3 - Article

VL - 9

SP - 216

EP - 223

JO - Journal of Natural Remedies

JF - Journal of Natural Remedies

SN - 0972-5547

IS - 2

ER -