Frozen-thawed spermatozoa from oligozoospermic ejaculates are susceptible to in situ DNA fragmentation in polyvinylpyrrolidone-based sperm-immobilization medium

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Objective: To elucidate the effect of sperm immobilization media that are and are not based on polyvinylpyrrolidone (PVP) on the DNA integrity of fresh and frozen-thawed spermatozoa during standard intracytoplasmic sperm injection (ICSI) conditions. Design: Experimental prospective study. Setting: Embryology research laboratory. Patient(s): Forty-six ejaculates from normozoospermic and oligozoospermic men. Intervention(s): Assessment of sperm DNA fragmentation by single-cell gel electrophoresis assay. Main Outcome Measure(s): DNA integrity of fresh and frozen-thawed spermatozoa from normozoospermic and oligozoospermic ejaculates exposed to PVP-based and non-PVP-based media. Result(s): Exposure of fresh and frozen thawed spermatozoa from normozoospermic and oligozoospermic ejaculates to PVP-based medium in an ICSI dish for 30 minutes statistically significantly increased the DNA fragmentation. In contrast, the extent of DNA fragmentation in non-PVP-based medium did not statistically significantly differ from control. Conclusion(s): A PVP-based medium can induce a statistically significant amount of sperm DNA fragmentation in an ICSI dish, and frozen-thawed sperm from oligozoospermic ejaculates are more susceptible to in situ DNA fragmentation.

Original languageEnglish
Pages (from-to)321-325
Number of pages5
JournalFertility and Sterility
Volume98
Issue number2
DOIs
Publication statusPublished - 08-2012

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Povidone
DNA Fragmentation
Immobilization
Spermatozoa
Intracytoplasmic Sperm Injections
Comet Assay
Embryology
DNA
Research Design
Outcome Assessment (Health Care)
Prospective Studies
Research

All Science Journal Classification (ASJC) codes

  • Obstetrics and Gynaecology
  • Reproductive Medicine

Cite this

@article{f673653ef39a4325ab8132721e2b4831,
title = "Frozen-thawed spermatozoa from oligozoospermic ejaculates are susceptible to in situ DNA fragmentation in polyvinylpyrrolidone-based sperm-immobilization medium",
abstract = "Objective: To elucidate the effect of sperm immobilization media that are and are not based on polyvinylpyrrolidone (PVP) on the DNA integrity of fresh and frozen-thawed spermatozoa during standard intracytoplasmic sperm injection (ICSI) conditions. Design: Experimental prospective study. Setting: Embryology research laboratory. Patient(s): Forty-six ejaculates from normozoospermic and oligozoospermic men. Intervention(s): Assessment of sperm DNA fragmentation by single-cell gel electrophoresis assay. Main Outcome Measure(s): DNA integrity of fresh and frozen-thawed spermatozoa from normozoospermic and oligozoospermic ejaculates exposed to PVP-based and non-PVP-based media. Result(s): Exposure of fresh and frozen thawed spermatozoa from normozoospermic and oligozoospermic ejaculates to PVP-based medium in an ICSI dish for 30 minutes statistically significantly increased the DNA fragmentation. In contrast, the extent of DNA fragmentation in non-PVP-based medium did not statistically significantly differ from control. Conclusion(s): A PVP-based medium can induce a statistically significant amount of sperm DNA fragmentation in an ICSI dish, and frozen-thawed sperm from oligozoospermic ejaculates are more susceptible to in situ DNA fragmentation.",
author = "Salian, {Sujit Raj} and Guruprasad Kalthur and Shubhashree Uppangala and Pratap Kumar and Adiga, {Satish Kumar}",
year = "2012",
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doi = "10.1016/j.fertnstert.2012.04.040",
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T1 - Frozen-thawed spermatozoa from oligozoospermic ejaculates are susceptible to in situ DNA fragmentation in polyvinylpyrrolidone-based sperm-immobilization medium

AU - Salian, Sujit Raj

AU - Kalthur, Guruprasad

AU - Uppangala, Shubhashree

AU - Kumar, Pratap

AU - Adiga, Satish Kumar

PY - 2012/8

Y1 - 2012/8

N2 - Objective: To elucidate the effect of sperm immobilization media that are and are not based on polyvinylpyrrolidone (PVP) on the DNA integrity of fresh and frozen-thawed spermatozoa during standard intracytoplasmic sperm injection (ICSI) conditions. Design: Experimental prospective study. Setting: Embryology research laboratory. Patient(s): Forty-six ejaculates from normozoospermic and oligozoospermic men. Intervention(s): Assessment of sperm DNA fragmentation by single-cell gel electrophoresis assay. Main Outcome Measure(s): DNA integrity of fresh and frozen-thawed spermatozoa from normozoospermic and oligozoospermic ejaculates exposed to PVP-based and non-PVP-based media. Result(s): Exposure of fresh and frozen thawed spermatozoa from normozoospermic and oligozoospermic ejaculates to PVP-based medium in an ICSI dish for 30 minutes statistically significantly increased the DNA fragmentation. In contrast, the extent of DNA fragmentation in non-PVP-based medium did not statistically significantly differ from control. Conclusion(s): A PVP-based medium can induce a statistically significant amount of sperm DNA fragmentation in an ICSI dish, and frozen-thawed sperm from oligozoospermic ejaculates are more susceptible to in situ DNA fragmentation.

AB - Objective: To elucidate the effect of sperm immobilization media that are and are not based on polyvinylpyrrolidone (PVP) on the DNA integrity of fresh and frozen-thawed spermatozoa during standard intracytoplasmic sperm injection (ICSI) conditions. Design: Experimental prospective study. Setting: Embryology research laboratory. Patient(s): Forty-six ejaculates from normozoospermic and oligozoospermic men. Intervention(s): Assessment of sperm DNA fragmentation by single-cell gel electrophoresis assay. Main Outcome Measure(s): DNA integrity of fresh and frozen-thawed spermatozoa from normozoospermic and oligozoospermic ejaculates exposed to PVP-based and non-PVP-based media. Result(s): Exposure of fresh and frozen thawed spermatozoa from normozoospermic and oligozoospermic ejaculates to PVP-based medium in an ICSI dish for 30 minutes statistically significantly increased the DNA fragmentation. In contrast, the extent of DNA fragmentation in non-PVP-based medium did not statistically significantly differ from control. Conclusion(s): A PVP-based medium can induce a statistically significant amount of sperm DNA fragmentation in an ICSI dish, and frozen-thawed sperm from oligozoospermic ejaculates are more susceptible to in situ DNA fragmentation.

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