Functional mimicry of the acetylated C-terminal tail of p53 by a SUMO-1 acetylated domain, SAD

Amrita Cheema, Chad D. Knights, Mahadev Rao, Jason Catania, Ricardo Perez, Brigitte Simons, Sivanesan Dakshanamurthy, Vamsi K. Kolukula, Maddalena Tilli, Priscilla A. Furth, Christopher Albanese, Maria Laura Avantaggiati

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The ubiquitin-like molecule, SUMO-1, a small protein essential for a variety of biological processes, is covalently conjugated to many intracellular proteins, especially to regulatory components of the transcriptional machinery, such as histones and transcription factors. Sumoylation provides either a stimulatory or an inhibitory signal for proliferation and for transcription, but the molecular mechanisms by which SUMO-1 achieves such versatility of effects are incompletely defined. The tumor suppressor and transcription regulator p53 is a relevant SUMO-1 target. Particularly, the C-terminal tail of p53 undergoes both sumoylation and acetylation. While the effects of sumoylation are still controversial, acetylation modifies p53 interaction with chromatin embedded promoters, and enforces p53 apoptotic activity. In this study, we show that the N-terminal region of SUMO-1 might functionally mimic this activity of the p53 C-terminal tail. We found that this SUMO-1 domain possesses similarity with the C-terminal acetylable p53 tail as well as with acetylable domains of other transcription factors. SUMO-1 is, indeed, acetylated when conjugated to its substrates and to p53. In the acetylable form SUMO-1 tunes the p53 response by modifying p53 transcriptional program, by promoting binding onto selected promoters and by favoring apoptosis. By contrast, when non-acetylable, SUMO-1 enforces cell-cycle arrest and p53 binding to a different sets of genes. These data demonstrate for the first time that SUMO-1, a post-translational modification is, in turn, modified by acetylation. Further, they imply that the pleiotropy of effects by which SUMO-1 influences various cellular outcomes and the activity of p53 depends upon its acetylation state.

Original languageEnglish
Pages (from-to)371-384
Number of pages14
JournalJournal of Cellular Physiology
Volume225
Issue number2
DOIs
Publication statusPublished - 2010

Fingerprint

Acetylation
Sumoylation
Transcription
Transcription Factors
Biological Phenomena
Post Translational Protein Processing
Ubiquitin
Cell Cycle Checkpoints
Histones
Chromatin
Machinery
Tumors
Proteins
Genes
Cells
Apoptosis
Molecules
Substrates
Neoplasms

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry
  • Cell Biology
  • Physiology
  • Medicine(all)

Cite this

Cheema, A., Knights, C. D., Rao, M., Catania, J., Perez, R., Simons, B., ... Avantaggiati, M. L. (2010). Functional mimicry of the acetylated C-terminal tail of p53 by a SUMO-1 acetylated domain, SAD. Journal of Cellular Physiology, 225(2), 371-384. https://doi.org/10.1002/jcp.22224
Cheema, Amrita ; Knights, Chad D. ; Rao, Mahadev ; Catania, Jason ; Perez, Ricardo ; Simons, Brigitte ; Dakshanamurthy, Sivanesan ; Kolukula, Vamsi K. ; Tilli, Maddalena ; Furth, Priscilla A. ; Albanese, Christopher ; Avantaggiati, Maria Laura. / Functional mimicry of the acetylated C-terminal tail of p53 by a SUMO-1 acetylated domain, SAD. In: Journal of Cellular Physiology. 2010 ; Vol. 225, No. 2. pp. 371-384.
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Cheema, A, Knights, CD, Rao, M, Catania, J, Perez, R, Simons, B, Dakshanamurthy, S, Kolukula, VK, Tilli, M, Furth, PA, Albanese, C & Avantaggiati, ML 2010, 'Functional mimicry of the acetylated C-terminal tail of p53 by a SUMO-1 acetylated domain, SAD', Journal of Cellular Physiology, vol. 225, no. 2, pp. 371-384. https://doi.org/10.1002/jcp.22224

Functional mimicry of the acetylated C-terminal tail of p53 by a SUMO-1 acetylated domain, SAD. / Cheema, Amrita; Knights, Chad D.; Rao, Mahadev; Catania, Jason; Perez, Ricardo; Simons, Brigitte; Dakshanamurthy, Sivanesan; Kolukula, Vamsi K.; Tilli, Maddalena; Furth, Priscilla A.; Albanese, Christopher; Avantaggiati, Maria Laura.

In: Journal of Cellular Physiology, Vol. 225, No. 2, 2010, p. 371-384.

Research output: Contribution to journalArticle

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T1 - Functional mimicry of the acetylated C-terminal tail of p53 by a SUMO-1 acetylated domain, SAD

AU - Cheema, Amrita

AU - Knights, Chad D.

AU - Rao, Mahadev

AU - Catania, Jason

AU - Perez, Ricardo

AU - Simons, Brigitte

AU - Dakshanamurthy, Sivanesan

AU - Kolukula, Vamsi K.

AU - Tilli, Maddalena

AU - Furth, Priscilla A.

AU - Albanese, Christopher

AU - Avantaggiati, Maria Laura

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