Fungal chondroitinase: Production and prospects for therapeutic application

Kasinathan Narayanan, Nagarathenam Sivagurunathan, Volety Mallikarjuna Subrahmanyam, Josyula Venkata Rao

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Chondroitinase is useful in treating glial scars and controlling tumour progression. Chondroitinase being a glycosylated protein, fungi could be an ideal host for production of chondroitinase. However, the work reported on bioprospecting of fungi capable of utilizing chondroitin sulphate and improvement of the cultural conditions is sparse. In this study, soil samples collected around various places of Udupi, India, were screened for isolating fungi capable of utilizing chondroitin sulphate and improving the enzyme yield. Seventy-six isolates obtained through soil enrichment were checked for chondroitinase activity by rapid plate technique. Among the 15 positive isolates, MSSS-1 was selected for further studies based on the isolate stability, consistency, yield and growth pattern. The enzyme yield was improved systematically through optimization of the cultural conditions, viz. one-factor-at-a-time method, factorial design andBox-Behnken design. The crude enzyme obtained by salt precipitation at 80% saturation controlled proliferation of HepG2, MCT-116 and MCF-7 cells. Maximum activity was observed against MCF-7 cells lines with an IC50 of 0.68 U/ml.

Original languageEnglish
Title of host publicationRecent advances in Applied Microbiology
PublisherSpringer Science+Business Media Singapore Private Limited
Pages275-290
Number of pages16
ISBN (Electronic)9789811052750
ISBN (Print)9789811052743
DOIs
Publication statusPublished - 07-11-2017

All Science Journal Classification (ASJC) codes

  • Medicine(all)
  • Health Professions(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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  • Cite this

    Narayanan, K., Sivagurunathan, N., Subrahmanyam, V. M., & Rao, J. V. (2017). Fungal chondroitinase: Production and prospects for therapeutic application. In Recent advances in Applied Microbiology (pp. 275-290). Springer Science+Business Media Singapore Private Limited. https://doi.org/10.1007/978-981-10-5275-0_14