Generation of integration free human iPSC line SORMi002-A from neonatal foreskin fibroblasts

Akshatha Shivaraj; Shagufta Parveen

doi:10.1016/j.scr.2019.101534

Generation of integration free human iPSC line SORMi002-A from neonatal foreskin fibroblasts

Akshatha Shivaraj, Shagufta Parveen

Manipal Institute of Regenerative Medicine, Bangalore

Research output: Contribution to journal › Article › peer-review

Abstract

Circumcision is a common surgical procedure usually performed on neonates worldwide. Skin removed after circumcision is abundant and often discarded. Neonatal foreskin fibroblasts (NFFs) can be easily isolated from it for application in biomedical research including induced pluripotent stem cell (iPSC) generation. In this study NFFs were reprogrammed to iPSCs using non-integrating episomal reprogramming plasmids. The iPSCs generated express pluripotency markers. They form embryoid bodies and differentiate in vitro into cells of endoderm, mesoderm and ectoderm. They have a normal male karyotype, are genetically identical to the parent fibroblasts and are transgene free.

Original language	English
Article number	101534
Journal	Stem Cell Research
Volume	40
DOIs	https://doi.org/10.1016/j.scr.2019.101534
Publication status	Published - 10-2019

All Science Journal Classification (ASJC) codes

Developmental Biology
Cell Biology

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abstract = "Circumcision is a common surgical procedure usually performed on neonates worldwide. Skin removed after circumcision is abundant and often discarded. Neonatal foreskin fibroblasts (NFFs) can be easily isolated from it for application in biomedical research including induced pluripotent stem cell (iPSC) generation. In this study NFFs were reprogrammed to iPSCs using non-integrating episomal reprogramming plasmids. The iPSCs generated express pluripotency markers. They form embryoid bodies and differentiate in vitro into cells of endoderm, mesoderm and ectoderm. They have a normal male karyotype, are genetically identical to the parent fibroblasts and are transgene free.",

author = "Akshatha Shivaraj and Shagufta Parveen",

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AB - Circumcision is a common surgical procedure usually performed on neonates worldwide. Skin removed after circumcision is abundant and often discarded. Neonatal foreskin fibroblasts (NFFs) can be easily isolated from it for application in biomedical research including induced pluripotent stem cell (iPSC) generation. In this study NFFs were reprogrammed to iPSCs using non-integrating episomal reprogramming plasmids. The iPSCs generated express pluripotency markers. They form embryoid bodies and differentiate in vitro into cells of endoderm, mesoderm and ectoderm. They have a normal male karyotype, are genetically identical to the parent fibroblasts and are transgene free.

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Generation of integration free human iPSC line SORMi002-A from neonatal foreskin fibroblasts

Abstract

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