Genetic diversity of Indian Plasmodium vivax isolates based on the analysis of PvMSP3β polymorphic marker

V. M. Anantabotla, Hiasindh Ashmi Antony, Noyal Maria Joseph, Subhash Chandra Parija, Nonika Rajkumari, Jyoti R. Kini, Radhakrishna Manipura, Vijaya Lakshmi Nag, R. S. Gadepalli, Nirupama Chayani, Somi Patro

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background: Malaria is one of the major communicable diseases in India and worldwide. PvMSP3β is a highly polymorphic gene due to its large insertions and deletions in the central alanine-rich region, which, in turn, makes it a valuable marker for population genetic analysis. Very few studies are available from India about the genetic diversity of Plasmodium vivax based on PvMSP3β gene, and hence, this study was designed to understand the molecular diversity of the P. vivax malaria parasite. The accumulating epidemiological data provide insights into the circulating genetic variants of P. vivax in India, and ultimately benefits the vaccine development. Materials and Methods: A total of 268 samples confirmed to be positive by microscopy, rapid diagnostic test, and quantitative buffy coat test were collected from four different regions of India (Puducherry, Mangaluru, Jodhpur, and Cuttack) in the present study. Polymerase chain reaction (PCR)-based diagnosis was carried out to confirm the P. vivax monoinfection, and only the mono-infected samples were subjected to PvMSP3β gene amplification and further restriction fragment length polymorphism (RFLP) to determine suballeles. Results: Based on the size of the amplified fragment, the PvMSP3β gene was apportioned into two major types, namely Type A genotype (1.6-2 Kb) was predominantly present in 148 isolates and Type B (1-1.5 Kb) was observed in 110 isolates. The percentage of mixed infections by PCR was 3.73%. All the PCR products were subjected to RFLP to categorize into suballeles and we detected 39 suballeles (A1-A39) in Type A, and 23 suballeles (B1-B23) in Type B genotype. A high degree of diversity was observed among the isolates collected from Mangaluru region when compared to isolates collected from other regions. Conclusion: The present study showed a high degree of genetic diversity of PvMSP3β gene among the isolates collected from various parts of India. High polymorphism in PvMSP3β gene makes it a promising marker for epidemiological and vaccine development studies.

Original languageEnglish
Pages (from-to)108-114
Number of pages7
JournalTropical Parasitology
Volume9
Issue number2
DOIs
Publication statusPublished - 01-07-2019

Fingerprint

Plasmodium vivax
India
Genes
Restriction Fragment Length Polymorphisms
Polymerase Chain Reaction
Marker Vaccines
Genotype
Vivax Malaria
Gene Amplification
Population Genetics
Coinfection
Routine Diagnostic Tests
Alanine
Malaria
Communicable Diseases
Microscopy
Parasites
Vaccines

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Microbiology
  • Infectious Diseases

Cite this

Anantabotla, V. M., Antony, H. A., Joseph, N. M., Parija, S. C., Rajkumari, N., Kini, J. R., ... Patro, S. (2019). Genetic diversity of Indian Plasmodium vivax isolates based on the analysis of PvMSP3β polymorphic marker. Tropical Parasitology, 9(2), 108-114. https://doi.org/10.4103/tp.TP_11_19
Anantabotla, V. M. ; Antony, Hiasindh Ashmi ; Joseph, Noyal Maria ; Parija, Subhash Chandra ; Rajkumari, Nonika ; Kini, Jyoti R. ; Manipura, Radhakrishna ; Nag, Vijaya Lakshmi ; Gadepalli, R. S. ; Chayani, Nirupama ; Patro, Somi. / Genetic diversity of Indian Plasmodium vivax isolates based on the analysis of PvMSP3β polymorphic marker. In: Tropical Parasitology. 2019 ; Vol. 9, No. 2. pp. 108-114.
@article{d4ed574b1a734cb9a33eb8e3fc4f801c,
title = "Genetic diversity of Indian Plasmodium vivax isolates based on the analysis of PvMSP3β polymorphic marker",
abstract = "Background: Malaria is one of the major communicable diseases in India and worldwide. PvMSP3β is a highly polymorphic gene due to its large insertions and deletions in the central alanine-rich region, which, in turn, makes it a valuable marker for population genetic analysis. Very few studies are available from India about the genetic diversity of Plasmodium vivax based on PvMSP3β gene, and hence, this study was designed to understand the molecular diversity of the P. vivax malaria parasite. The accumulating epidemiological data provide insights into the circulating genetic variants of P. vivax in India, and ultimately benefits the vaccine development. Materials and Methods: A total of 268 samples confirmed to be positive by microscopy, rapid diagnostic test, and quantitative buffy coat test were collected from four different regions of India (Puducherry, Mangaluru, Jodhpur, and Cuttack) in the present study. Polymerase chain reaction (PCR)-based diagnosis was carried out to confirm the P. vivax monoinfection, and only the mono-infected samples were subjected to PvMSP3β gene amplification and further restriction fragment length polymorphism (RFLP) to determine suballeles. Results: Based on the size of the amplified fragment, the PvMSP3β gene was apportioned into two major types, namely Type A genotype (1.6-2 Kb) was predominantly present in 148 isolates and Type B (1-1.5 Kb) was observed in 110 isolates. The percentage of mixed infections by PCR was 3.73{\%}. All the PCR products were subjected to RFLP to categorize into suballeles and we detected 39 suballeles (A1-A39) in Type A, and 23 suballeles (B1-B23) in Type B genotype. A high degree of diversity was observed among the isolates collected from Mangaluru region when compared to isolates collected from other regions. Conclusion: The present study showed a high degree of genetic diversity of PvMSP3β gene among the isolates collected from various parts of India. High polymorphism in PvMSP3β gene makes it a promising marker for epidemiological and vaccine development studies.",
author = "Anantabotla, {V. M.} and Antony, {Hiasindh Ashmi} and Joseph, {Noyal Maria} and Parija, {Subhash Chandra} and Nonika Rajkumari and Kini, {Jyoti R.} and Radhakrishna Manipura and Nag, {Vijaya Lakshmi} and Gadepalli, {R. S.} and Nirupama Chayani and Somi Patro",
year = "2019",
month = "7",
day = "1",
doi = "10.4103/tp.TP_11_19",
language = "English",
volume = "9",
pages = "108--114",
journal = "Tropical Parasitology",
issn = "2229-5070",
publisher = "Medknow Publications and Media Pvt. Ltd",
number = "2",

}

Anantabotla, VM, Antony, HA, Joseph, NM, Parija, SC, Rajkumari, N, Kini, JR, Manipura, R, Nag, VL, Gadepalli, RS, Chayani, N & Patro, S 2019, 'Genetic diversity of Indian Plasmodium vivax isolates based on the analysis of PvMSP3β polymorphic marker', Tropical Parasitology, vol. 9, no. 2, pp. 108-114. https://doi.org/10.4103/tp.TP_11_19

Genetic diversity of Indian Plasmodium vivax isolates based on the analysis of PvMSP3β polymorphic marker. / Anantabotla, V. M.; Antony, Hiasindh Ashmi; Joseph, Noyal Maria; Parija, Subhash Chandra; Rajkumari, Nonika; Kini, Jyoti R.; Manipura, Radhakrishna; Nag, Vijaya Lakshmi; Gadepalli, R. S.; Chayani, Nirupama; Patro, Somi.

In: Tropical Parasitology, Vol. 9, No. 2, 01.07.2019, p. 108-114.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Genetic diversity of Indian Plasmodium vivax isolates based on the analysis of PvMSP3β polymorphic marker

AU - Anantabotla, V. M.

AU - Antony, Hiasindh Ashmi

AU - Joseph, Noyal Maria

AU - Parija, Subhash Chandra

AU - Rajkumari, Nonika

AU - Kini, Jyoti R.

AU - Manipura, Radhakrishna

AU - Nag, Vijaya Lakshmi

AU - Gadepalli, R. S.

AU - Chayani, Nirupama

AU - Patro, Somi

PY - 2019/7/1

Y1 - 2019/7/1

N2 - Background: Malaria is one of the major communicable diseases in India and worldwide. PvMSP3β is a highly polymorphic gene due to its large insertions and deletions in the central alanine-rich region, which, in turn, makes it a valuable marker for population genetic analysis. Very few studies are available from India about the genetic diversity of Plasmodium vivax based on PvMSP3β gene, and hence, this study was designed to understand the molecular diversity of the P. vivax malaria parasite. The accumulating epidemiological data provide insights into the circulating genetic variants of P. vivax in India, and ultimately benefits the vaccine development. Materials and Methods: A total of 268 samples confirmed to be positive by microscopy, rapid diagnostic test, and quantitative buffy coat test were collected from four different regions of India (Puducherry, Mangaluru, Jodhpur, and Cuttack) in the present study. Polymerase chain reaction (PCR)-based diagnosis was carried out to confirm the P. vivax monoinfection, and only the mono-infected samples were subjected to PvMSP3β gene amplification and further restriction fragment length polymorphism (RFLP) to determine suballeles. Results: Based on the size of the amplified fragment, the PvMSP3β gene was apportioned into two major types, namely Type A genotype (1.6-2 Kb) was predominantly present in 148 isolates and Type B (1-1.5 Kb) was observed in 110 isolates. The percentage of mixed infections by PCR was 3.73%. All the PCR products were subjected to RFLP to categorize into suballeles and we detected 39 suballeles (A1-A39) in Type A, and 23 suballeles (B1-B23) in Type B genotype. A high degree of diversity was observed among the isolates collected from Mangaluru region when compared to isolates collected from other regions. Conclusion: The present study showed a high degree of genetic diversity of PvMSP3β gene among the isolates collected from various parts of India. High polymorphism in PvMSP3β gene makes it a promising marker for epidemiological and vaccine development studies.

AB - Background: Malaria is one of the major communicable diseases in India and worldwide. PvMSP3β is a highly polymorphic gene due to its large insertions and deletions in the central alanine-rich region, which, in turn, makes it a valuable marker for population genetic analysis. Very few studies are available from India about the genetic diversity of Plasmodium vivax based on PvMSP3β gene, and hence, this study was designed to understand the molecular diversity of the P. vivax malaria parasite. The accumulating epidemiological data provide insights into the circulating genetic variants of P. vivax in India, and ultimately benefits the vaccine development. Materials and Methods: A total of 268 samples confirmed to be positive by microscopy, rapid diagnostic test, and quantitative buffy coat test were collected from four different regions of India (Puducherry, Mangaluru, Jodhpur, and Cuttack) in the present study. Polymerase chain reaction (PCR)-based diagnosis was carried out to confirm the P. vivax monoinfection, and only the mono-infected samples were subjected to PvMSP3β gene amplification and further restriction fragment length polymorphism (RFLP) to determine suballeles. Results: Based on the size of the amplified fragment, the PvMSP3β gene was apportioned into two major types, namely Type A genotype (1.6-2 Kb) was predominantly present in 148 isolates and Type B (1-1.5 Kb) was observed in 110 isolates. The percentage of mixed infections by PCR was 3.73%. All the PCR products were subjected to RFLP to categorize into suballeles and we detected 39 suballeles (A1-A39) in Type A, and 23 suballeles (B1-B23) in Type B genotype. A high degree of diversity was observed among the isolates collected from Mangaluru region when compared to isolates collected from other regions. Conclusion: The present study showed a high degree of genetic diversity of PvMSP3β gene among the isolates collected from various parts of India. High polymorphism in PvMSP3β gene makes it a promising marker for epidemiological and vaccine development studies.

UR - http://www.scopus.com/inward/record.url?scp=85072644388&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85072644388&partnerID=8YFLogxK

U2 - 10.4103/tp.TP_11_19

DO - 10.4103/tp.TP_11_19

M3 - Article

VL - 9

SP - 108

EP - 114

JO - Tropical Parasitology

JF - Tropical Parasitology

SN - 2229-5070

IS - 2

ER -