Genotype-phenotype association of TGF-β1 and GST with chemo-radiotherapy induced toxicity

H. V. Goutham, K. D. Mumbrekar, N. Hitendra, B. M. Vadhiraja, D. J. Fernandes, B. S. Satish Rao

Research output: Contribution to journalArticle

Abstract

Background: Normal tissue toxicity continues to remain as a major challenge for radiation oncologists for delivering the total dose to the tumour cells in cancer patients. Cellular, molecular and plasma based early biomarkers to predict the overreactions and non-overreactions of normal tissue toxicity before the initiation of radiotherapy can be valuable for personalised treatment. The aim of the current study was to analyse the interrelationship between polymorphisms in Glutathione S- Transferases (GSTs) and Transforming Growth Factor-β1 (TGF-β1), the plasma level/activity of these proteins with the development of chemo-radiotherapy induced oral mucositis and skin reaction in head and neck cancer (HNC) patients. Materials and Methods: We analysed polymorphisms in TGF-β1 and GST by restriction digestion of the PCR amplified products and we also assessed circulating TGF- β1 levels and GST activity by Enzyme Linked Immunosorbent Assay (ELISA). Results: The results indicate that pre-radiotherapy plasma TGF-β1 levels and total GST activity has no correlation with radiation induced normal tissue skin reaction and oral mucositis in HNC patients. Conclusion: The selected polymorphisms in TGF-β1 and GST had no influence on TGF-β1 levels and total GST activity. Plasma TGF-β1 and GST activity was not affected by the presence of selected polymorphisms and lacks significance in predicting skin reaction and oral mucositis prior to chemo-radiotherapy.

Original languageEnglish
Pages (from-to)15-23
Number of pages9
JournalInternational Journal of Radiation Research
Volume15
Issue number1
DOIs
Publication statusPublished - 01-01-2017

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Genetic Association Studies
Transforming Growth Factors
Glutathione Transferase
Radiotherapy
Stomatitis
Head and Neck Neoplasms
Skin
Digestion
Neoplasms
Biomarkers
Enzyme-Linked Immunosorbent Assay
Radiation
Polymerase Chain Reaction
Proteins

All Science Journal Classification (ASJC) codes

  • Radiological and Ultrasound Technology
  • Radiology Nuclear Medicine and imaging

Cite this

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title = "Genotype-phenotype association of TGF-β1 and GST with chemo-radiotherapy induced toxicity",
abstract = "Background: Normal tissue toxicity continues to remain as a major challenge for radiation oncologists for delivering the total dose to the tumour cells in cancer patients. Cellular, molecular and plasma based early biomarkers to predict the overreactions and non-overreactions of normal tissue toxicity before the initiation of radiotherapy can be valuable for personalised treatment. The aim of the current study was to analyse the interrelationship between polymorphisms in Glutathione S- Transferases (GSTs) and Transforming Growth Factor-β1 (TGF-β1), the plasma level/activity of these proteins with the development of chemo-radiotherapy induced oral mucositis and skin reaction in head and neck cancer (HNC) patients. Materials and Methods: We analysed polymorphisms in TGF-β1 and GST by restriction digestion of the PCR amplified products and we also assessed circulating TGF- β1 levels and GST activity by Enzyme Linked Immunosorbent Assay (ELISA). Results: The results indicate that pre-radiotherapy plasma TGF-β1 levels and total GST activity has no correlation with radiation induced normal tissue skin reaction and oral mucositis in HNC patients. Conclusion: The selected polymorphisms in TGF-β1 and GST had no influence on TGF-β1 levels and total GST activity. Plasma TGF-β1 and GST activity was not affected by the presence of selected polymorphisms and lacks significance in predicting skin reaction and oral mucositis prior to chemo-radiotherapy.",
author = "Goutham, {H. V.} and Mumbrekar, {K. D.} and N. Hitendra and Vadhiraja, {B. M.} and Fernandes, {D. J.} and {Satish Rao}, {B. S.}",
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Genotype-phenotype association of TGF-β1 and GST with chemo-radiotherapy induced toxicity. / Goutham, H. V.; Mumbrekar, K. D.; Hitendra, N.; Vadhiraja, B. M.; Fernandes, D. J.; Satish Rao, B. S.

In: International Journal of Radiation Research, Vol. 15, No. 1, 01.01.2017, p. 15-23.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Genotype-phenotype association of TGF-β1 and GST with chemo-radiotherapy induced toxicity

AU - Goutham, H. V.

AU - Mumbrekar, K. D.

AU - Hitendra, N.

AU - Vadhiraja, B. M.

AU - Fernandes, D. J.

AU - Satish Rao, B. S.

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Background: Normal tissue toxicity continues to remain as a major challenge for radiation oncologists for delivering the total dose to the tumour cells in cancer patients. Cellular, molecular and plasma based early biomarkers to predict the overreactions and non-overreactions of normal tissue toxicity before the initiation of radiotherapy can be valuable for personalised treatment. The aim of the current study was to analyse the interrelationship between polymorphisms in Glutathione S- Transferases (GSTs) and Transforming Growth Factor-β1 (TGF-β1), the plasma level/activity of these proteins with the development of chemo-radiotherapy induced oral mucositis and skin reaction in head and neck cancer (HNC) patients. Materials and Methods: We analysed polymorphisms in TGF-β1 and GST by restriction digestion of the PCR amplified products and we also assessed circulating TGF- β1 levels and GST activity by Enzyme Linked Immunosorbent Assay (ELISA). Results: The results indicate that pre-radiotherapy plasma TGF-β1 levels and total GST activity has no correlation with radiation induced normal tissue skin reaction and oral mucositis in HNC patients. Conclusion: The selected polymorphisms in TGF-β1 and GST had no influence on TGF-β1 levels and total GST activity. Plasma TGF-β1 and GST activity was not affected by the presence of selected polymorphisms and lacks significance in predicting skin reaction and oral mucositis prior to chemo-radiotherapy.

AB - Background: Normal tissue toxicity continues to remain as a major challenge for radiation oncologists for delivering the total dose to the tumour cells in cancer patients. Cellular, molecular and plasma based early biomarkers to predict the overreactions and non-overreactions of normal tissue toxicity before the initiation of radiotherapy can be valuable for personalised treatment. The aim of the current study was to analyse the interrelationship between polymorphisms in Glutathione S- Transferases (GSTs) and Transforming Growth Factor-β1 (TGF-β1), the plasma level/activity of these proteins with the development of chemo-radiotherapy induced oral mucositis and skin reaction in head and neck cancer (HNC) patients. Materials and Methods: We analysed polymorphisms in TGF-β1 and GST by restriction digestion of the PCR amplified products and we also assessed circulating TGF- β1 levels and GST activity by Enzyme Linked Immunosorbent Assay (ELISA). Results: The results indicate that pre-radiotherapy plasma TGF-β1 levels and total GST activity has no correlation with radiation induced normal tissue skin reaction and oral mucositis in HNC patients. Conclusion: The selected polymorphisms in TGF-β1 and GST had no influence on TGF-β1 levels and total GST activity. Plasma TGF-β1 and GST activity was not affected by the presence of selected polymorphisms and lacks significance in predicting skin reaction and oral mucositis prior to chemo-radiotherapy.

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