Genotypic variations of Porphyromonas gingivalis in chronic periodontitis patients with and without diabetes

An in vitro study using arbitrarily primed-polymerase chain reaction and heteroduplex-polymerase chain reaction

Siladitya Sen, Kukkamalla Meena Anand

Research output: Contribution to journalArticle

Abstract

Context: Diabetes has become an endemic throughout the world. Periodontitis is associated with diabetes, and it has been regarded as the sixth complication of diabetes. However, the role of diabetes on periodontopathic microbiota such as Porphyromonas gingivalis remains unclear. Aims: To compare the total number of different genotypes of P. gingivalis in chronic periodontitis patients with and without diabetes by using arbitrarily primed-polymerase chain reaction (AP-PCR) and heteroduplex-PCR. Settings and Design: This is a single-center comparative study. Materials and Methods: A total of 100 patients were included in this study. Patients were divided into two groups: Group A - patients having chronic periodontitis without diabetes (50) and Group B - patients having chronic periodontitis with diabetes (50). Subgingival plaque samples were collected from both groups of patients. Plaque samples were cultured for P. gingivalis. Positive culture samples and extracted DNA from samples by proteinase-K method were collected. Part of DNA samples were checked by AP-PCR using OPA-13 primer, and part of the DNA were checked using heteroduplex-PCR using P. gingivalis-specific Pg8 primer. Results were analyzed by polyacrylamide gel electrophoresis procedure. Statistical Analysis Used: Independent t-test and Chi-square test (SPSS 16) were used. Results: Thirty-four out of fifty samples of Group B patients were found positive for P. gingivalis, whereas 21 out of 50 samples in Group A were found positive for P. gingivalis. AP-PCR showed nine different genotypes in Group B and six different genotypes in Group A. Heteroduplex-PCR showed a total of seven different genotypes in Group B and five different genotypes in Group A. Conclusions: Results of this study show that, in diabetic condition, P. gingivalis shows increased variations in genotypes.

Original languageEnglish
Pages (from-to)409-415
Number of pages7
JournalJournal of Indian Society of Periodontology
Volume23
Issue number5
DOIs
Publication statusPublished - 01-09-2019
Externally publishedYes

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Chronic Periodontitis
Porphyromonas gingivalis
Genotype
Polymerase Chain Reaction
Endopeptidase K
DNA Primers
Periodontitis
Microbiota
DNA
Diabetes Complications
Chi-Square Distribution
In Vitro Techniques
Polyacrylamide Gel Electrophoresis

All Science Journal Classification (ASJC) codes

  • Periodontics

Cite this

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title = "Genotypic variations of Porphyromonas gingivalis in chronic periodontitis patients with and without diabetes: An in vitro study using arbitrarily primed-polymerase chain reaction and heteroduplex-polymerase chain reaction",
abstract = "Context: Diabetes has become an endemic throughout the world. Periodontitis is associated with diabetes, and it has been regarded as the sixth complication of diabetes. However, the role of diabetes on periodontopathic microbiota such as Porphyromonas gingivalis remains unclear. Aims: To compare the total number of different genotypes of P. gingivalis in chronic periodontitis patients with and without diabetes by using arbitrarily primed-polymerase chain reaction (AP-PCR) and heteroduplex-PCR. Settings and Design: This is a single-center comparative study. Materials and Methods: A total of 100 patients were included in this study. Patients were divided into two groups: Group A - patients having chronic periodontitis without diabetes (50) and Group B - patients having chronic periodontitis with diabetes (50). Subgingival plaque samples were collected from both groups of patients. Plaque samples were cultured for P. gingivalis. Positive culture samples and extracted DNA from samples by proteinase-K method were collected. Part of DNA samples were checked by AP-PCR using OPA-13 primer, and part of the DNA were checked using heteroduplex-PCR using P. gingivalis-specific Pg8 primer. Results were analyzed by polyacrylamide gel electrophoresis procedure. Statistical Analysis Used: Independent t-test and Chi-square test (SPSS 16) were used. Results: Thirty-four out of fifty samples of Group B patients were found positive for P. gingivalis, whereas 21 out of 50 samples in Group A were found positive for P. gingivalis. AP-PCR showed nine different genotypes in Group B and six different genotypes in Group A. Heteroduplex-PCR showed a total of seven different genotypes in Group B and five different genotypes in Group A. Conclusions: Results of this study show that, in diabetic condition, P. gingivalis shows increased variations in genotypes.",
author = "Siladitya Sen and Anand, {Kukkamalla Meena}",
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TY - JOUR

T1 - Genotypic variations of Porphyromonas gingivalis in chronic periodontitis patients with and without diabetes

T2 - An in vitro study using arbitrarily primed-polymerase chain reaction and heteroduplex-polymerase chain reaction

AU - Sen, Siladitya

AU - Anand, Kukkamalla Meena

PY - 2019/9/1

Y1 - 2019/9/1

N2 - Context: Diabetes has become an endemic throughout the world. Periodontitis is associated with diabetes, and it has been regarded as the sixth complication of diabetes. However, the role of diabetes on periodontopathic microbiota such as Porphyromonas gingivalis remains unclear. Aims: To compare the total number of different genotypes of P. gingivalis in chronic periodontitis patients with and without diabetes by using arbitrarily primed-polymerase chain reaction (AP-PCR) and heteroduplex-PCR. Settings and Design: This is a single-center comparative study. Materials and Methods: A total of 100 patients were included in this study. Patients were divided into two groups: Group A - patients having chronic periodontitis without diabetes (50) and Group B - patients having chronic periodontitis with diabetes (50). Subgingival plaque samples were collected from both groups of patients. Plaque samples were cultured for P. gingivalis. Positive culture samples and extracted DNA from samples by proteinase-K method were collected. Part of DNA samples were checked by AP-PCR using OPA-13 primer, and part of the DNA were checked using heteroduplex-PCR using P. gingivalis-specific Pg8 primer. Results were analyzed by polyacrylamide gel electrophoresis procedure. Statistical Analysis Used: Independent t-test and Chi-square test (SPSS 16) were used. Results: Thirty-four out of fifty samples of Group B patients were found positive for P. gingivalis, whereas 21 out of 50 samples in Group A were found positive for P. gingivalis. AP-PCR showed nine different genotypes in Group B and six different genotypes in Group A. Heteroduplex-PCR showed a total of seven different genotypes in Group B and five different genotypes in Group A. Conclusions: Results of this study show that, in diabetic condition, P. gingivalis shows increased variations in genotypes.

AB - Context: Diabetes has become an endemic throughout the world. Periodontitis is associated with diabetes, and it has been regarded as the sixth complication of diabetes. However, the role of diabetes on periodontopathic microbiota such as Porphyromonas gingivalis remains unclear. Aims: To compare the total number of different genotypes of P. gingivalis in chronic periodontitis patients with and without diabetes by using arbitrarily primed-polymerase chain reaction (AP-PCR) and heteroduplex-PCR. Settings and Design: This is a single-center comparative study. Materials and Methods: A total of 100 patients were included in this study. Patients were divided into two groups: Group A - patients having chronic periodontitis without diabetes (50) and Group B - patients having chronic periodontitis with diabetes (50). Subgingival plaque samples were collected from both groups of patients. Plaque samples were cultured for P. gingivalis. Positive culture samples and extracted DNA from samples by proteinase-K method were collected. Part of DNA samples were checked by AP-PCR using OPA-13 primer, and part of the DNA were checked using heteroduplex-PCR using P. gingivalis-specific Pg8 primer. Results were analyzed by polyacrylamide gel electrophoresis procedure. Statistical Analysis Used: Independent t-test and Chi-square test (SPSS 16) were used. Results: Thirty-four out of fifty samples of Group B patients were found positive for P. gingivalis, whereas 21 out of 50 samples in Group A were found positive for P. gingivalis. AP-PCR showed nine different genotypes in Group B and six different genotypes in Group A. Heteroduplex-PCR showed a total of seven different genotypes in Group B and five different genotypes in Group A. Conclusions: Results of this study show that, in diabetic condition, P. gingivalis shows increased variations in genotypes.

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U2 - 10.4103/jisp.jisp_406_18

DO - 10.4103/jisp.jisp_406_18

M3 - Article

VL - 23

SP - 409

EP - 415

JO - Journal of Indian Society of Periodontology

JF - Journal of Indian Society of Periodontology

SN - 0972-124X

IS - 5

ER -