Germ cell abnormalities in streptozotocin induced diabetic mice do not correlate with blood glucose level

Rohini Bose, Satish K. Adiga, Fiona D'Souza, Sujith R. Salian, Shubhashree Uppangala, Guruprasad Kalthur, Navya Jain, Raghu A. Radhakrishnan, Nalini Bhat, Hanumantappa Krishnamurthy, Pratap Kumar

Research output: Contribution to journalArticle

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Abstract

Purpose: To assess the effect of streptozotocin induced hyperglycemia on germ cell integrity, DNA ploidy and methylation status for a period of two spermatogenesis cycles in adult male Swiss albino mice. Methods: Streptozotocin injected mice were monitored for hyperglycemia at a regular interval for a period of 36 and 72 days. The DNA integrity in epididymal spermatozoa was determined by the comet assay. Flow cytometric analysis was done in germ cells to assess the DNA ploidy. The global methylation analysis in germ cells was done by 5-methyl cytosine immunostaining. Results: Streptozotocin administration successfully resulted in hyperglycemic response which significantly affected serum testosterone level, sperm DNA integrity and DNA ploidy at the end of 36 days. However, no changes were observed in either epididymal sperm concentration or germ cell methylation status. In contrast, at the end of 76 days, although serum testosterone level, sperm DNA integrity and DNA ploidy status were unperturbed significantly in hyperglycemic group, the epididymal sperm concentration and methylation status of preleptotene/zygotene cells were significantly altered. Importantly, an attempt to find out the association between the blood glucose levels and the abnormalities in hyperglycemic group failed to demonstrate any correlation. Conclusions: The germ cell abnormalities observed in hyperglycemic group could be interpreted as a primary effect of streptozotocin and not due to hyperglycemia. Our results call for further evaluation of streptozotocin before its application to study the hyperglycemic responses on male germ cells.

Original languageEnglish
Pages (from-to)1405-1413
Number of pages9
JournalJournal of Assisted Reproduction and Genetics
Volume29
Issue number12
DOIs
Publication statusPublished - 12-2012

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Streptozocin
Germ Cells
Blood Glucose
Ploidies
Spermatozoa
DNA
Hyperglycemia
Methylation
Testosterone
Comet Assay
Cytosine
DNA Methylation
Spermatogenesis
Serum

All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Genetics
  • Obstetrics and Gynaecology
  • Developmental Biology
  • Genetics(clinical)

Cite this

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title = "Germ cell abnormalities in streptozotocin induced diabetic mice do not correlate with blood glucose level",
abstract = "Purpose: To assess the effect of streptozotocin induced hyperglycemia on germ cell integrity, DNA ploidy and methylation status for a period of two spermatogenesis cycles in adult male Swiss albino mice. Methods: Streptozotocin injected mice were monitored for hyperglycemia at a regular interval for a period of 36 and 72 days. The DNA integrity in epididymal spermatozoa was determined by the comet assay. Flow cytometric analysis was done in germ cells to assess the DNA ploidy. The global methylation analysis in germ cells was done by 5-methyl cytosine immunostaining. Results: Streptozotocin administration successfully resulted in hyperglycemic response which significantly affected serum testosterone level, sperm DNA integrity and DNA ploidy at the end of 36 days. However, no changes were observed in either epididymal sperm concentration or germ cell methylation status. In contrast, at the end of 76 days, although serum testosterone level, sperm DNA integrity and DNA ploidy status were unperturbed significantly in hyperglycemic group, the epididymal sperm concentration and methylation status of preleptotene/zygotene cells were significantly altered. Importantly, an attempt to find out the association between the blood glucose levels and the abnormalities in hyperglycemic group failed to demonstrate any correlation. Conclusions: The germ cell abnormalities observed in hyperglycemic group could be interpreted as a primary effect of streptozotocin and not due to hyperglycemia. Our results call for further evaluation of streptozotocin before its application to study the hyperglycemic responses on male germ cells.",
author = "Rohini Bose and Adiga, {Satish K.} and Fiona D'Souza and Salian, {Sujith R.} and Shubhashree Uppangala and Guruprasad Kalthur and Navya Jain and Radhakrishnan, {Raghu A.} and Nalini Bhat and Hanumantappa Krishnamurthy and Pratap Kumar",
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Germ cell abnormalities in streptozotocin induced diabetic mice do not correlate with blood glucose level. / Bose, Rohini; Adiga, Satish K.; D'Souza, Fiona; Salian, Sujith R.; Uppangala, Shubhashree; Kalthur, Guruprasad; Jain, Navya; Radhakrishnan, Raghu A.; Bhat, Nalini; Krishnamurthy, Hanumantappa; Kumar, Pratap.

In: Journal of Assisted Reproduction and Genetics, Vol. 29, No. 12, 12.2012, p. 1405-1413.

Research output: Contribution to journalArticle

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T1 - Germ cell abnormalities in streptozotocin induced diabetic mice do not correlate with blood glucose level

AU - Bose, Rohini

AU - Adiga, Satish K.

AU - D'Souza, Fiona

AU - Salian, Sujith R.

AU - Uppangala, Shubhashree

AU - Kalthur, Guruprasad

AU - Jain, Navya

AU - Radhakrishnan, Raghu A.

AU - Bhat, Nalini

AU - Krishnamurthy, Hanumantappa

AU - Kumar, Pratap

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N2 - Purpose: To assess the effect of streptozotocin induced hyperglycemia on germ cell integrity, DNA ploidy and methylation status for a period of two spermatogenesis cycles in adult male Swiss albino mice. Methods: Streptozotocin injected mice were monitored for hyperglycemia at a regular interval for a period of 36 and 72 days. The DNA integrity in epididymal spermatozoa was determined by the comet assay. Flow cytometric analysis was done in germ cells to assess the DNA ploidy. The global methylation analysis in germ cells was done by 5-methyl cytosine immunostaining. Results: Streptozotocin administration successfully resulted in hyperglycemic response which significantly affected serum testosterone level, sperm DNA integrity and DNA ploidy at the end of 36 days. However, no changes were observed in either epididymal sperm concentration or germ cell methylation status. In contrast, at the end of 76 days, although serum testosterone level, sperm DNA integrity and DNA ploidy status were unperturbed significantly in hyperglycemic group, the epididymal sperm concentration and methylation status of preleptotene/zygotene cells were significantly altered. Importantly, an attempt to find out the association between the blood glucose levels and the abnormalities in hyperglycemic group failed to demonstrate any correlation. Conclusions: The germ cell abnormalities observed in hyperglycemic group could be interpreted as a primary effect of streptozotocin and not due to hyperglycemia. Our results call for further evaluation of streptozotocin before its application to study the hyperglycemic responses on male germ cells.

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