Haemolytic effects of hypo-osmotic salt solutions on human erythrocytes

O. Nepal, J. P. Rao

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: While it is well known that hypotonic solutions of sodium chloride induce hemolysis, the effects of other salt solutions on human erythrocytes have not been well documented. Objective: The study is to compare the effects of other salt solutions on human red cells. Methods: Iso-osmotic and hypo-osmotic solutions of various salts were prepared after taking into account their molecular weight and osmotic pressure. Five healthy volunteers between the age of 22-30 years were randomly selected and ten blood samples were collected from them. The study was conducted from January 2009 to February 2009. Blood was collected from subjects by venepuncture into heparinised tubes. 20 μl of blood was pipetted into 1 ml of each solution and incubated for one hour at 37°C in a water bath. The solutions were centrifuged and the colour of the supernatant was read in a spectrophotometer. Supernatant from blood added to distilled water was considered 100% hemolysed. Results: Iso-osmotic salt solutions were free of hemolysis. Among chloride salts, sodium chloride showed the least hemolysis and potassium chloride and nickel chloride resulted into greater hemolysis. Among potassium salts, potassium bromate caused highest amount of hemolysis whereas potassium sulphate showed the least. Conclusion: The significant differences in hemolytic pattern in hypo-osmotic salts solutions suggest that the hypo-osmotic stress causes morphological changes in red cells that alter their permeability to various ions leading to hemolysis. This probably occurs through opening of volume sensitive channels.

Original languageEnglish
Pages (from-to)35-39
Number of pages5
JournalKathmandu University Medical Journal
Volume9
Issue number34
Publication statusPublished - 01-04-2011
Externally publishedYes

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Hemolysis
Salts
Erythrocytes
Osmotic Pressure
Sodium Chloride
Hypotonic Solutions
Phlebotomy
Potassium Chloride
Water
Baths
Chlorides
Permeability
Healthy Volunteers
Potassium
Color
Molecular Weight
Ions

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

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abstract = "Background: While it is well known that hypotonic solutions of sodium chloride induce hemolysis, the effects of other salt solutions on human erythrocytes have not been well documented. Objective: The study is to compare the effects of other salt solutions on human red cells. Methods: Iso-osmotic and hypo-osmotic solutions of various salts were prepared after taking into account their molecular weight and osmotic pressure. Five healthy volunteers between the age of 22-30 years were randomly selected and ten blood samples were collected from them. The study was conducted from January 2009 to February 2009. Blood was collected from subjects by venepuncture into heparinised tubes. 20 μl of blood was pipetted into 1 ml of each solution and incubated for one hour at 37°C in a water bath. The solutions were centrifuged and the colour of the supernatant was read in a spectrophotometer. Supernatant from blood added to distilled water was considered 100{\%} hemolysed. Results: Iso-osmotic salt solutions were free of hemolysis. Among chloride salts, sodium chloride showed the least hemolysis and potassium chloride and nickel chloride resulted into greater hemolysis. Among potassium salts, potassium bromate caused highest amount of hemolysis whereas potassium sulphate showed the least. Conclusion: The significant differences in hemolytic pattern in hypo-osmotic salts solutions suggest that the hypo-osmotic stress causes morphological changes in red cells that alter their permeability to various ions leading to hemolysis. This probably occurs through opening of volume sensitive channels.",
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Haemolytic effects of hypo-osmotic salt solutions on human erythrocytes. / Nepal, O.; Rao, J. P.

In: Kathmandu University Medical Journal, Vol. 9, No. 34, 01.04.2011, p. 35-39.

Research output: Contribution to journalArticle

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