Hemocompatible curcumin-dextran micelles as pH sensitive pro-drugs for enhanced therapeutic efficacy in cancer cells

Radhika Raveendran, G. S. Bhuvaneshwar, Chandra P. Sharma

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Curcumin, a component in spice turmeric, is renowned to possess anti-cancer therapeutic potential. However, low aqueous solubility and instability of curcumin which subsequently affects its bioavailability pose as major impediments in its translation to clinical application. In this regard, we focused on conjugating hydrophobic curcumin to the hydrophilic backbone of dextran via succinic acid spacer to design a pro-drug. The structural confirmation of the conjugates was carried out using FTIR and 1H NMR spectroscopy. Critical micelle measurement affirmed the micelle formation of the pro-drug in aqueous media. The size distribution and zeta potential of the curcumin-dextran (Cur-Dex) micelles were determined using dynamic light scattering technique. The micellar architecture bestowed curcumin negligible susceptibility to degradation under physiological conditions along with enhanced aqueous solubility. Biocompatibility of the micelles was proved by the blood component aggregation and plasma protein interaction studies. In vitro release studies demonstrated the pH sensitivity release of curcumin which is conducive to the tumour micro environment. Profound cytotoxic effects of Cur-Dex micelles in C6 glioma cells were observed from MTT and Live/Dead assay experiments. Moreover, enhanced cellular internalization of the Cur-Dex micelles compared to free curcumin in the cancer cells was revealed by fluorescence microscopy. Our study focuses on the feasibility of Cur-Dex micelles to be extrapolated as promising candidates for safe and efficient cancer therapy.

Original languageEnglish
Pages (from-to)497-507
Number of pages11
JournalCarbohydrate Polymers
Volume137
DOIs
Publication statusPublished - 10-02-2016
Externally publishedYes

Fingerprint

Curcumin
Dextran
Prodrugs
Micelles
Dextrans
Cells
Solubility
Fluorescence microscopy
Beam plasma interactions
Dynamic light scattering
Zeta potential
Biocompatibility
Nuclear magnetic resonance spectroscopy
Tumors
Assays
Blood
Agglomeration
Succinic Acid
Proteins
Plasmas

All Science Journal Classification (ASJC) codes

  • Polymers and Plastics
  • Organic Chemistry
  • Materials Chemistry

Cite this

Raveendran, Radhika ; Bhuvaneshwar, G. S. ; Sharma, Chandra P. / Hemocompatible curcumin-dextran micelles as pH sensitive pro-drugs for enhanced therapeutic efficacy in cancer cells. In: Carbohydrate Polymers. 2016 ; Vol. 137. pp. 497-507.
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Hemocompatible curcumin-dextran micelles as pH sensitive pro-drugs for enhanced therapeutic efficacy in cancer cells. / Raveendran, Radhika; Bhuvaneshwar, G. S.; Sharma, Chandra P.

In: Carbohydrate Polymers, Vol. 137, 10.02.2016, p. 497-507.

Research output: Contribution to journalArticle

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