TY - JOUR
T1 - He–Ne laser accelerates seed germination by modulating growth hormones and reprogramming metabolism in brinjal
AU - Swathy, Puthanvila Surendrababu
AU - Kiran, Kodsara Ramachandra
AU - Joshi, Manjunath B.
AU - Mahato, Krishna Kishore
AU - Muthusamy, Annamalai
N1 - Funding Information:
We are grateful to Manipal Academy of Higher Education (MAHE), Manipal, Karnataka, India, TIFAC-CORE, Department of Science and Technology (DST), Government of India, and VGST, Government of Karnataka for the facilities. We are thankful to DAE-BRNS (Ref No. 2012/34/47/BRNS) for the laser facility. We are obliged to Manipal Academy of Higher Education (MAHE), Manipal for Dr. T.M.A. Pai Ph.D. scholarship to Swathy PS and Kiran KR. We are indebted to Prof. K. Satyamoorthy, Director, Manipal School of Life Sciences, Manipal Academy of Higher Education, Manipal, India for his encouragement and support. We would like to thank Mrs. Shashikala Tantry, Mr. Subhash Chandra and Mr. Manoj Kumar KK, for their experimental assistance. The authors are obliged to Prof. Radhakrishna Rao for his constant suggestions, Shri. Mohan Rao, Mattu Gulla Growers Association and Shri. Lakshmana Rao, Mattu Village, Udupi, Karnataka, India for providing the seeds to carry out the study. We would like to express our gratitude to Editorial board members and reviewers for their constructive and critical comments to improve the earlier version of the manuscript. We are indebted to Ms. Arya K and Mr. Sachin T for their help in the format of the manuscript.
Publisher Copyright:
© 2021, The Author(s).
PY - 2021/12
Y1 - 2021/12
N2 - A plant’s ability to maximize seed germination, growth, and photosynthetic productivity depends on its aptitude to sense, evaluate, and respond to the quality, quantity, and direction of the light. Among diverse colors of light possessing different wavelengths and red light shown to have a high impact on the photosynthetic and growth responses of the plants. The use of artificial light sources where the quality, intensity, and duration of exposure can be controlled would be an efficient method to increase the efficiency of the crop plants. The coherent, collimated, and monochromatic properties of laser light sources enabled as biostimulator compared to the normal light. The present study was attempted to use the potential role of the He–Ne laser as a bio-stimulator device to improve the germination and growth of brinjal and to investigate the possible interactions of plant and laser photons. A substantial enhancement was observed in germination index, germination time and seed vigor index of laser-irradiated than control groups. The enhanced germination rate was correlated with higher GA content and its biosynthetic genes whereas decreased ABA content and its catabolic genes and GA/ABA ratio were noted in laser-irradiated groups during seed germination than control groups. Further the expression of phytochrome gene transcripts, PhyA and PhyB1 were upregulated in laser-irradiated seedlings which correlate with enhanced seed germination than control. Elevated levels of primary metabolites were noted in the early stages of germination whereas modulation of secondary metabolites was observed in later growth. Consequently, significantly increased photosynthetic rate, stomatal conductance, and transpiration rate was perceived in laser-irradiated seedlings compare with control. The current study showed hormone and phytochrome-mediated mechanisms of seed germination in laser-irradiated groups along with the enhanced photosynthetic rate, primary and secondary metabolites.
AB - A plant’s ability to maximize seed germination, growth, and photosynthetic productivity depends on its aptitude to sense, evaluate, and respond to the quality, quantity, and direction of the light. Among diverse colors of light possessing different wavelengths and red light shown to have a high impact on the photosynthetic and growth responses of the plants. The use of artificial light sources where the quality, intensity, and duration of exposure can be controlled would be an efficient method to increase the efficiency of the crop plants. The coherent, collimated, and monochromatic properties of laser light sources enabled as biostimulator compared to the normal light. The present study was attempted to use the potential role of the He–Ne laser as a bio-stimulator device to improve the germination and growth of brinjal and to investigate the possible interactions of plant and laser photons. A substantial enhancement was observed in germination index, germination time and seed vigor index of laser-irradiated than control groups. The enhanced germination rate was correlated with higher GA content and its biosynthetic genes whereas decreased ABA content and its catabolic genes and GA/ABA ratio were noted in laser-irradiated groups during seed germination than control groups. Further the expression of phytochrome gene transcripts, PhyA and PhyB1 were upregulated in laser-irradiated seedlings which correlate with enhanced seed germination than control. Elevated levels of primary metabolites were noted in the early stages of germination whereas modulation of secondary metabolites was observed in later growth. Consequently, significantly increased photosynthetic rate, stomatal conductance, and transpiration rate was perceived in laser-irradiated seedlings compare with control. The current study showed hormone and phytochrome-mediated mechanisms of seed germination in laser-irradiated groups along with the enhanced photosynthetic rate, primary and secondary metabolites.
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UR - http://www.scopus.com/inward/citedby.url?scp=85104230414&partnerID=8YFLogxK
U2 - 10.1038/s41598-021-86984-8
DO - 10.1038/s41598-021-86984-8
M3 - Article
C2 - 33846419
AN - SCOPUS:85104230414
SN - 2045-2322
VL - 11
JO - Scientific Reports
JF - Scientific Reports
IS - 1
M1 - 7948
ER -