Hepatoprotective evaluation of Anogeissus latifolia: In vitro and in vivo studies

H.A. Pradeep, S. Khan, K. Ravikumar, M.F. Ahmed, M.N.A Rao, M. Kiranmai, D.S. Reddy, S.R. Ahamed, M. Ibrahim

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

AIM: To evaluate the hepatoprotective activity of a hydroalcoholic extract of the bark of Anogeissus latifolia ; in vitro in primary rat hepatocyte monolayer culture and in vivo in the liver of Wistar rats intoxicated by carbon tetrachloride (CCl4). METHODS: In the in vitro study, a primary hepatocyte monolayer culture was treated with CCl4 and extract of Anogeissus latifolia . Hepatoprotective activity was demonstrated in the CCl4 damaged primary monolayer culture. In the in vivo study, the hepatoprotective activity of a hydroalcoholic extract of Anogeissus latifolia was analyzed in liver injured CCl4-treated rats. Biochemical parameters including serum transaminases [aspartate aminotransferase (AST) and alanine aminotransferase (ALT)] and alkaline phosphatase (ALP) in serum were analyzed. The biochemical findings were supplemented with histopathological examination of rat liver sections. RESULTS: In vitro : primary hepatocyte monolayer cultures were treated with CCl4 and extract of Anogeissus latifolia . A protective activity could be demonstrated in the CCl4 damaged primary monolayer culture. In vivo : Hydroalcoholic extract of Anogeissus latifolia (300 mg/kg) was found to have protective activity in rats with CCl4-induced liver damage as judged from serum marker enzyme activity. CONCLUSION: The above findings lead to the conclusion that the hydroalcoholic extract of Anogeissus latifolia is hepatoprotective. Hence, we suggest that the inclusion of this plant in the management of liver disorders is justified. © 2009 The WJG Press and Baishideng. All rights reserved.
Original languageEnglish
Pages (from-to)4816-4822
Number of pages7
JournalWorld Journal of Gastroenterology
Volume15
Issue number38
DOIs
Publication statusPublished - 2009

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Liver
Hepatocytes
Carbon Tetrachloride
Aspartate Aminotransferases
Transaminases
Serum
Alanine Transaminase
Alkaline Phosphatase
Wistar Rats
Biomarkers
In Vitro Techniques
Enzymes

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Pradeep, H. A., Khan, S., Ravikumar, K., Ahmed, M. F., Rao, M. N. A., Kiranmai, M., ... Ibrahim, M. (2009). Hepatoprotective evaluation of Anogeissus latifolia: In vitro and in vivo studies. World Journal of Gastroenterology, 15(38), 4816-4822. https://doi.org/10.3748/wjg.15.4816
Pradeep, H.A. ; Khan, S. ; Ravikumar, K. ; Ahmed, M.F. ; Rao, M.N.A ; Kiranmai, M. ; Reddy, D.S. ; Ahamed, S.R. ; Ibrahim, M. / Hepatoprotective evaluation of Anogeissus latifolia: In vitro and in vivo studies. In: World Journal of Gastroenterology. 2009 ; Vol. 15, No. 38. pp. 4816-4822.
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title = "Hepatoprotective evaluation of Anogeissus latifolia: In vitro and in vivo studies",
abstract = "AIM: To evaluate the hepatoprotective activity of a hydroalcoholic extract of the bark of Anogeissus latifolia ; in vitro in primary rat hepatocyte monolayer culture and in vivo in the liver of Wistar rats intoxicated by carbon tetrachloride (CCl4). METHODS: In the in vitro study, a primary hepatocyte monolayer culture was treated with CCl4 and extract of Anogeissus latifolia . Hepatoprotective activity was demonstrated in the CCl4 damaged primary monolayer culture. In the in vivo study, the hepatoprotective activity of a hydroalcoholic extract of Anogeissus latifolia was analyzed in liver injured CCl4-treated rats. Biochemical parameters including serum transaminases [aspartate aminotransferase (AST) and alanine aminotransferase (ALT)] and alkaline phosphatase (ALP) in serum were analyzed. The biochemical findings were supplemented with histopathological examination of rat liver sections. RESULTS: In vitro : primary hepatocyte monolayer cultures were treated with CCl4 and extract of Anogeissus latifolia . A protective activity could be demonstrated in the CCl4 damaged primary monolayer culture. In vivo : Hydroalcoholic extract of Anogeissus latifolia (300 mg/kg) was found to have protective activity in rats with CCl4-induced liver damage as judged from serum marker enzyme activity. CONCLUSION: The above findings lead to the conclusion that the hydroalcoholic extract of Anogeissus latifolia is hepatoprotective. Hence, we suggest that the inclusion of this plant in the management of liver disorders is justified. {\circledC} 2009 The WJG Press and Baishideng. All rights reserved.",
author = "H.A. Pradeep and S. Khan and K. Ravikumar and M.F. Ahmed and M.N.A Rao and M. Kiranmai and D.S. Reddy and S.R. Ahamed and M. Ibrahim",
note = "Cited By :25 Export Date: 10 November 2017 CODEN: WJGAF Correspondence Address: Ibrahim, M.; Department of Pharmacology and Biotechnology, Nizam Institute of Pharmacy, Near Ramoji Film City, Nalgonda 508284, Andhra Pradesh, India; email: ibrahim_cce@rediffmail.com Chemicals/CAS: alanine aminotransferase, 9000-86-6, 9014-30-6; alcohol, 64-17-5; alkaline phosphatase, 9001-78-9; aspartate aminotransferase, 9000-97-9; carbon tetrachloride, 56-23-5; liv 52, 54847-94-8; water, 7732-18-5; Alanine Transaminase, 2.6.1.2; Alkaline Phosphatase, 3.1.3.1; Aspartate Aminotransferases, 2.6.1.1; Carbon Tetrachloride, 56-23-5; Plant Extracts Manufacturers: Himalaya, India References: Stehbens, W.E., Oxidative stress, toxic hepatitis, and antioxidants with particular emphasis on zinc (2003) Exp Mol Pathol, 75, pp. 265-276; Jaeschke, H., Knight, T.R., Bajt, M.L., The role of oxidant stress and reactive nitrogen species in acetaminophen hepatotoxicity (2003) Toxicol Lett, 144, pp. 279-288; McDonough, K.H., Antioxidant nutrients and alcohol (2003) Toxicology, 189, pp. 89-97; Jaeschke, H., Smith, C.V., Mitchell, J.R., Reactive oxygen species during ischemia-reflow injury in isolated perfused rat liver (1988) J Clin Invest, 81, pp. 1240-1246; Fang, Y.Z., Yang, S., Wu, G., Free radicals, antioxidants, and nutrition (2002) Nutrition, 18, pp. 872-879; Gupta, M., Mazumder, U.K., Sambath, K.R., Antioxidant and protective effect of Ervatamia coronaria Stapf leaves against carbon tetrachloride induced liver injury (2004) European Bull Drug Res, 12, pp. 13-22; Gupta, M., Mazumder, U.K., Siva, K.T., Antioxidant and hepatoprotective effect of Bauhinia racemosa against paracetamol and CCl4 induced liver damage in rats (2004) Iranian J Pharmacol Therapeutics, 3, pp. 12-20; Kukongviriyapan, V., Janyacharoen, T., Kukongviriyapan, U., Laupattarakasaem, P., Kanokmedhakul, S., Chantaranothai, P., Hepatoprotective and antioxidant activities of Tetracera loureiri (2003) Phytother Res, 17, pp. 717-721; New Delhi (1985) Anonymous, Pharmacognosy of Indigenous drugs, 1, pp. 250-259. , Central council for Research in Ayurveda and Siddha; Govindarajan, R., Vijayakumar, M., Rao, C.V., Shirwaikar, A., Rawat, A.K., Mehrotra, S., Pushpangadan, P., Antioxidant potential of Anogeissus latifolia (2004) Biol Pharm Bull, 27, pp. 1266-1269; Reddy, K.K., Rajadurai, S., Nayudamma, Y., Studies on Dhava (Anogeissus latifolia) Tannins: Part III- Polyphenols of bark, sapwood and heartwood of Dhava (1965) Indian J Chem, pp. 308-310; Deshpande VH, Patil AD, Ramarao AV, Venkataraman K. 3,3'-Di-O-methylellagic Acid-4'-β-D-xyloside and 3,4,3'-tri-O- methylflavellagic acid-4'-β-D-glucoside from Anogeissus latifolia bark. Indian J Chem 1976; 14B: 641-643; Aruoma, O.I., Murcia, A., Butler, J., Halliwell, B., Evaluation of the antioxidant and prooxidant actions of gallic acid and its derivatives (1993) J Agric Food Chem, 41, pp. 1880-1885; Festa, F., Aglitti, T., Duranti, G., Ricordy, R., Perticone, P., Cozzi, R., Strong antioxidant activity of ellagic acid in mammalian cells in vitro revealed by the comet assay (2001) Anticancer Res, 21, pp. 3903-3908; Boyle, S.P., Dobson, V.L., Duthie, S.J., Hinselwood, D.C., Kyle, J.A., Collins, A.R., Bioavailability and efficiency of rutin as an antioxidant: A human supplementation study (2000) Eur J Clin Nutr, 54, pp. 774-782; Boots, A.W., Haenen, G.R., Bast, A., Health effects of quercetin: From antioxidant to nutraceutical (2008) Eur J Pharmacol, 585, pp. 325-337; Govindarajan, R., Vijayakumar, M., Singh, M., Rao, C.V., Shirwaikar, A., Rawat, A.K., Pushpangadan, P., Antiulcer and antimicrobial activity of Anogeissus latifolia (2006) J Ethnopharmacol, 106, pp. 57-61; Govindarajan, R., Vijayakumar, M., Rao, C.V., Shirwaikar, A., Mehrotra, S., Pushpangadan, P., Healing potential of Anogeissus latifolia for dermal wounds in rats (2004) Acta Pharm, 54, pp. 331-338; Janbaz, K.H., Saeed, S.A., Gilani, A.H., Protective effect of rutin on paracetamol- and CCl4-induced hepatotoxicity in rodents (2002) Fitoterapia, 73, pp. 557-563; Janbaz, K.H., Saeed, S.A., Gi lani, A.H., Studies on the protective effects of caffeic acid and quercetin on chemicalinduced hepatotoxicity in rodents (2004) Phytomedicine, 11, pp. 424-430; Khan, S., Pradeep, H.A., Vijan, R., Chemoprotective activity of Anogeissus latifolia bark extract on Paracetamol-induced hepatotoxicity (2008) Pharmacologyonline, 2, pp. 303-327; Mills, S., Bone, K., Principles and practice of phytotherapy (2000) Churchill Livingstone, 35, pp. 220-222; Sadasivam, S., Manickam, A., (1992) Biochemical methods for Agricultural Sciences, pp. 187-190. , New Delhi: Wiley Eastern Ltd; Woisky, R., Salationo, A., Analysis of propils: Some parameters and procedures for chemical and quality control (1998) J Apicultural Research, 37, pp. 99-105; Kaufman PB, Cseke LJ, Sarawarber, Duke JA, Brielmamm HL. Natural products from plants. New York: CRC Press, 1999: 20-22; Wang, X., Lous, Z., Mikage, M., Namba, T., Pharmacognostical studies on the Chinese crude drug da-huang rhubarb II. Botanical origin of three unofficial da-huang (1988) Shoyakugaku Zasshi, 42, pp. 302-309; Vogel, G., (1977) New natural products and Plant drugs with Pharmacological, Biological and Therapeutical activity, pp. 249-265. , Berlin: Springer Verlag; Mukerjee, A.B., Dasgupta, M., Treatment of viral Hepatitis B an indegenious drug Liv 52 (1970) Ind Pract, 6, p. 357; Tasaduq, S.A., Singh, K., Sethi, S., Sharma, S.C., Bedi, K.L., Singh, J., Jaggi, B.S., Johri, R.K., Hepatocurative and antioxidant profile of HP-1, a polyherbal phytomedicine (2003) Hum Exp Toxicol, 22, pp. 639-645; Seglen, P.O., Preparation of isolated rat liver cells (1976) Methods Cell Biol, 13, pp. 29-83; Tingstrom, A., Obrink, B., Distribution and dynamics of cell surface-associated cellCAM 105 in cultured rat hepatocytes (1989) Exp Cell Res, 185, pp. 132-142; Kiso, Y., Tohkin, M., Hikino, H., Assay method for antihepatotoxic activity using galactosamine-induced cytotoxicity in primary-cultured hepatocytes (1983) J Nat Prod, 46, pp. 841-847; Ecobichon, D.J., (1997) The basis of toxicology testing, pp. 43-86. , New York: CRC Press; Niehaus, W.G., Samuelson, B., Formation of malondialdehyde from phospholipids arachidonate during microsomal lipid peroxidation (1968) Eur J Biochem, 17, pp. 126-130; Noroozi, M., Angerson, W.J., Lean, M.E., Effects of flavonoids and vitamin C on oxidative DNA damage to human lymphocytes (1998) Am J Clin Nutr, 67, pp. 1210-1218; Afanas'ev, I.B., Dorozhko, A.I., Brodskii, A.V., Kostyuk, V.A., Potapovitch, A.I., Chelating and free radical scavenging mechanisms of inhibitory action of rutin and quercetin in lipid peroxidation (1989) Biochem Pharmacol, 38, pp. 1763-1769; Recknagacl, R., Carbontetrachloride hepatotoxicity (1967) Pharmacological Reviews, 19, pp. 145-196; Mulander, D.W., Wrublewski, F., La Due, J.S., Transaminase compared with cholinesterase and alkaline phosphatase an index of hepatocellular integrity (1955) Clinical Research Proceedings, 3, pp. 20-24; Labib, R., Turkall, R., Abdel-Rahman, M.S., Endotoxin potentiates cocaine-mediated hepatotoxicity by nitric oxide and reactive oxygen species (2003) Int J Toxicol, 22, pp. 305-316; Sohn, D.H., Kim, Y.C., Oh, S.H., Park, E.J., Li, X., Lee, B.H., Hepatoprotective and free radical scavenging effects of Nelumbo nucifera (2003) Phytomedicine, 10, pp. 165-169; Gao, H., Zhou, Y.W., Anti-lipid peroxidation and protection of liver mitochondria against injuries by picroside II (2005) World J Gastroenterol, 11, pp. 3671-3674; Bear, W.L., Teel, R.W., Effects of citrus phytochemicals on liver and lung cytochrome P450 activity and on the in vitro metabolism of the tobacco-specific nitrosamine NNK (2000) Anticancer Res, 20, pp. 3323-3329",
year = "2009",
doi = "10.3748/wjg.15.4816",
language = "English",
volume = "15",
pages = "4816--4822",
journal = "World Journal of Gastroenterology",
issn = "1007-9327",
publisher = "WJG Press",
number = "38",

}

Pradeep, HA, Khan, S, Ravikumar, K, Ahmed, MF, Rao, MNA, Kiranmai, M, Reddy, DS, Ahamed, SR & Ibrahim, M 2009, 'Hepatoprotective evaluation of Anogeissus latifolia: In vitro and in vivo studies', World Journal of Gastroenterology, vol. 15, no. 38, pp. 4816-4822. https://doi.org/10.3748/wjg.15.4816

Hepatoprotective evaluation of Anogeissus latifolia: In vitro and in vivo studies. / Pradeep, H.A.; Khan, S.; Ravikumar, K.; Ahmed, M.F.; Rao, M.N.A; Kiranmai, M.; Reddy, D.S.; Ahamed, S.R.; Ibrahim, M.

In: World Journal of Gastroenterology, Vol. 15, No. 38, 2009, p. 4816-4822.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Hepatoprotective evaluation of Anogeissus latifolia: In vitro and in vivo studies

AU - Pradeep, H.A.

AU - Khan, S.

AU - Ravikumar, K.

AU - Ahmed, M.F.

AU - Rao, M.N.A

AU - Kiranmai, M.

AU - Reddy, D.S.

AU - Ahamed, S.R.

AU - Ibrahim, M.

N1 - Cited By :25 Export Date: 10 November 2017 CODEN: WJGAF Correspondence Address: Ibrahim, M.; Department of Pharmacology and Biotechnology, Nizam Institute of Pharmacy, Near Ramoji Film City, Nalgonda 508284, Andhra Pradesh, India; email: ibrahim_cce@rediffmail.com Chemicals/CAS: alanine aminotransferase, 9000-86-6, 9014-30-6; alcohol, 64-17-5; alkaline phosphatase, 9001-78-9; aspartate aminotransferase, 9000-97-9; carbon tetrachloride, 56-23-5; liv 52, 54847-94-8; water, 7732-18-5; Alanine Transaminase, 2.6.1.2; Alkaline Phosphatase, 3.1.3.1; Aspartate Aminotransferases, 2.6.1.1; Carbon Tetrachloride, 56-23-5; Plant Extracts Manufacturers: Himalaya, India References: Stehbens, W.E., Oxidative stress, toxic hepatitis, and antioxidants with particular emphasis on zinc (2003) Exp Mol Pathol, 75, pp. 265-276; Jaeschke, H., Knight, T.R., Bajt, M.L., The role of oxidant stress and reactive nitrogen species in acetaminophen hepatotoxicity (2003) Toxicol Lett, 144, pp. 279-288; McDonough, K.H., Antioxidant nutrients and alcohol (2003) Toxicology, 189, pp. 89-97; Jaeschke, H., Smith, C.V., Mitchell, J.R., Reactive oxygen species during ischemia-reflow injury in isolated perfused rat liver (1988) J Clin Invest, 81, pp. 1240-1246; Fang, Y.Z., Yang, S., Wu, G., Free radicals, antioxidants, and nutrition (2002) Nutrition, 18, pp. 872-879; Gupta, M., Mazumder, U.K., Sambath, K.R., Antioxidant and protective effect of Ervatamia coronaria Stapf leaves against carbon tetrachloride induced liver injury (2004) European Bull Drug Res, 12, pp. 13-22; Gupta, M., Mazumder, U.K., Siva, K.T., Antioxidant and hepatoprotective effect of Bauhinia racemosa against paracetamol and CCl4 induced liver damage in rats (2004) Iranian J Pharmacol Therapeutics, 3, pp. 12-20; Kukongviriyapan, V., Janyacharoen, T., Kukongviriyapan, U., Laupattarakasaem, P., Kanokmedhakul, S., Chantaranothai, P., Hepatoprotective and antioxidant activities of Tetracera loureiri (2003) Phytother Res, 17, pp. 717-721; New Delhi (1985) Anonymous, Pharmacognosy of Indigenous drugs, 1, pp. 250-259. , Central council for Research in Ayurveda and Siddha; Govindarajan, R., Vijayakumar, M., Rao, C.V., Shirwaikar, A., Rawat, A.K., Mehrotra, S., Pushpangadan, P., Antioxidant potential of Anogeissus latifolia (2004) Biol Pharm Bull, 27, pp. 1266-1269; Reddy, K.K., Rajadurai, S., Nayudamma, Y., Studies on Dhava (Anogeissus latifolia) Tannins: Part III- Polyphenols of bark, sapwood and heartwood of Dhava (1965) Indian J Chem, pp. 308-310; Deshpande VH, Patil AD, Ramarao AV, Venkataraman K. 3,3'-Di-O-methylellagic Acid-4'-β-D-xyloside and 3,4,3'-tri-O- methylflavellagic acid-4'-β-D-glucoside from Anogeissus latifolia bark. Indian J Chem 1976; 14B: 641-643; Aruoma, O.I., Murcia, A., Butler, J., Halliwell, B., Evaluation of the antioxidant and prooxidant actions of gallic acid and its derivatives (1993) J Agric Food Chem, 41, pp. 1880-1885; Festa, F., Aglitti, T., Duranti, G., Ricordy, R., Perticone, P., Cozzi, R., Strong antioxidant activity of ellagic acid in mammalian cells in vitro revealed by the comet assay (2001) Anticancer Res, 21, pp. 3903-3908; Boyle, S.P., Dobson, V.L., Duthie, S.J., Hinselwood, D.C., Kyle, J.A., Collins, A.R., Bioavailability and efficiency of rutin as an antioxidant: A human supplementation study (2000) Eur J Clin Nutr, 54, pp. 774-782; Boots, A.W., Haenen, G.R., Bast, A., Health effects of quercetin: From antioxidant to nutraceutical (2008) Eur J Pharmacol, 585, pp. 325-337; Govindarajan, R., Vijayakumar, M., Singh, M., Rao, C.V., Shirwaikar, A., Rawat, A.K., Pushpangadan, P., Antiulcer and antimicrobial activity of Anogeissus latifolia (2006) J Ethnopharmacol, 106, pp. 57-61; Govindarajan, R., Vijayakumar, M., Rao, C.V., Shirwaikar, A., Mehrotra, S., Pushpangadan, P., Healing potential of Anogeissus latifolia for dermal wounds in rats (2004) Acta Pharm, 54, pp. 331-338; Janbaz, K.H., Saeed, S.A., Gilani, A.H., Protective effect of rutin on paracetamol- and CCl4-induced hepatotoxicity in rodents (2002) Fitoterapia, 73, pp. 557-563; Janbaz, K.H., Saeed, S.A., Gi lani, A.H., Studies on the protective effects of caffeic acid and quercetin on chemicalinduced hepatotoxicity in rodents (2004) Phytomedicine, 11, pp. 424-430; Khan, S., Pradeep, H.A., Vijan, R., Chemoprotective activity of Anogeissus latifolia bark extract on Paracetamol-induced hepatotoxicity (2008) Pharmacologyonline, 2, pp. 303-327; Mills, S., Bone, K., Principles and practice of phytotherapy (2000) Churchill Livingstone, 35, pp. 220-222; Sadasivam, S., Manickam, A., (1992) Biochemical methods for Agricultural Sciences, pp. 187-190. , New Delhi: Wiley Eastern Ltd; Woisky, R., Salationo, A., Analysis of propils: Some parameters and procedures for chemical and quality control (1998) J Apicultural Research, 37, pp. 99-105; Kaufman PB, Cseke LJ, Sarawarber, Duke JA, Brielmamm HL. Natural products from plants. New York: CRC Press, 1999: 20-22; Wang, X., Lous, Z., Mikage, M., Namba, T., Pharmacognostical studies on the Chinese crude drug da-huang rhubarb II. Botanical origin of three unofficial da-huang (1988) Shoyakugaku Zasshi, 42, pp. 302-309; Vogel, G., (1977) New natural products and Plant drugs with Pharmacological, Biological and Therapeutical activity, pp. 249-265. , Berlin: Springer Verlag; Mukerjee, A.B., Dasgupta, M., Treatment of viral Hepatitis B an indegenious drug Liv 52 (1970) Ind Pract, 6, p. 357; Tasaduq, S.A., Singh, K., Sethi, S., Sharma, S.C., Bedi, K.L., Singh, J., Jaggi, B.S., Johri, R.K., Hepatocurative and antioxidant profile of HP-1, a polyherbal phytomedicine (2003) Hum Exp Toxicol, 22, pp. 639-645; Seglen, P.O., Preparation of isolated rat liver cells (1976) Methods Cell Biol, 13, pp. 29-83; Tingstrom, A., Obrink, B., Distribution and dynamics of cell surface-associated cellCAM 105 in cultured rat hepatocytes (1989) Exp Cell Res, 185, pp. 132-142; Kiso, Y., Tohkin, M., Hikino, H., Assay method for antihepatotoxic activity using galactosamine-induced cytotoxicity in primary-cultured hepatocytes (1983) J Nat Prod, 46, pp. 841-847; Ecobichon, D.J., (1997) The basis of toxicology testing, pp. 43-86. , New York: CRC Press; Niehaus, W.G., Samuelson, B., Formation of malondialdehyde from phospholipids arachidonate during microsomal lipid peroxidation (1968) Eur J Biochem, 17, pp. 126-130; Noroozi, M., Angerson, W.J., Lean, M.E., Effects of flavonoids and vitamin C on oxidative DNA damage to human lymphocytes (1998) Am J Clin Nutr, 67, pp. 1210-1218; Afanas'ev, I.B., Dorozhko, A.I., Brodskii, A.V., Kostyuk, V.A., Potapovitch, A.I., Chelating and free radical scavenging mechanisms of inhibitory action of rutin and quercetin in lipid peroxidation (1989) Biochem Pharmacol, 38, pp. 1763-1769; Recknagacl, R., Carbontetrachloride hepatotoxicity (1967) Pharmacological Reviews, 19, pp. 145-196; Mulander, D.W., Wrublewski, F., La Due, J.S., Transaminase compared with cholinesterase and alkaline phosphatase an index of hepatocellular integrity (1955) Clinical Research Proceedings, 3, pp. 20-24; Labib, R., Turkall, R., Abdel-Rahman, M.S., Endotoxin potentiates cocaine-mediated hepatotoxicity by nitric oxide and reactive oxygen species (2003) Int J Toxicol, 22, pp. 305-316; Sohn, D.H., Kim, Y.C., Oh, S.H., Park, E.J., Li, X., Lee, B.H., Hepatoprotective and free radical scavenging effects of Nelumbo nucifera (2003) Phytomedicine, 10, pp. 165-169; Gao, H., Zhou, Y.W., Anti-lipid peroxidation and protection of liver mitochondria against injuries by picroside II (2005) World J Gastroenterol, 11, pp. 3671-3674; Bear, W.L., Teel, R.W., Effects of citrus phytochemicals on liver and lung cytochrome P450 activity and on the in vitro metabolism of the tobacco-specific nitrosamine NNK (2000) Anticancer Res, 20, pp. 3323-3329

PY - 2009

Y1 - 2009

N2 - AIM: To evaluate the hepatoprotective activity of a hydroalcoholic extract of the bark of Anogeissus latifolia ; in vitro in primary rat hepatocyte monolayer culture and in vivo in the liver of Wistar rats intoxicated by carbon tetrachloride (CCl4). METHODS: In the in vitro study, a primary hepatocyte monolayer culture was treated with CCl4 and extract of Anogeissus latifolia . Hepatoprotective activity was demonstrated in the CCl4 damaged primary monolayer culture. In the in vivo study, the hepatoprotective activity of a hydroalcoholic extract of Anogeissus latifolia was analyzed in liver injured CCl4-treated rats. Biochemical parameters including serum transaminases [aspartate aminotransferase (AST) and alanine aminotransferase (ALT)] and alkaline phosphatase (ALP) in serum were analyzed. The biochemical findings were supplemented with histopathological examination of rat liver sections. RESULTS: In vitro : primary hepatocyte monolayer cultures were treated with CCl4 and extract of Anogeissus latifolia . A protective activity could be demonstrated in the CCl4 damaged primary monolayer culture. In vivo : Hydroalcoholic extract of Anogeissus latifolia (300 mg/kg) was found to have protective activity in rats with CCl4-induced liver damage as judged from serum marker enzyme activity. CONCLUSION: The above findings lead to the conclusion that the hydroalcoholic extract of Anogeissus latifolia is hepatoprotective. Hence, we suggest that the inclusion of this plant in the management of liver disorders is justified. © 2009 The WJG Press and Baishideng. All rights reserved.

AB - AIM: To evaluate the hepatoprotective activity of a hydroalcoholic extract of the bark of Anogeissus latifolia ; in vitro in primary rat hepatocyte monolayer culture and in vivo in the liver of Wistar rats intoxicated by carbon tetrachloride (CCl4). METHODS: In the in vitro study, a primary hepatocyte monolayer culture was treated with CCl4 and extract of Anogeissus latifolia . Hepatoprotective activity was demonstrated in the CCl4 damaged primary monolayer culture. In the in vivo study, the hepatoprotective activity of a hydroalcoholic extract of Anogeissus latifolia was analyzed in liver injured CCl4-treated rats. Biochemical parameters including serum transaminases [aspartate aminotransferase (AST) and alanine aminotransferase (ALT)] and alkaline phosphatase (ALP) in serum were analyzed. The biochemical findings were supplemented with histopathological examination of rat liver sections. RESULTS: In vitro : primary hepatocyte monolayer cultures were treated with CCl4 and extract of Anogeissus latifolia . A protective activity could be demonstrated in the CCl4 damaged primary monolayer culture. In vivo : Hydroalcoholic extract of Anogeissus latifolia (300 mg/kg) was found to have protective activity in rats with CCl4-induced liver damage as judged from serum marker enzyme activity. CONCLUSION: The above findings lead to the conclusion that the hydroalcoholic extract of Anogeissus latifolia is hepatoprotective. Hence, we suggest that the inclusion of this plant in the management of liver disorders is justified. © 2009 The WJG Press and Baishideng. All rights reserved.

U2 - 10.3748/wjg.15.4816

DO - 10.3748/wjg.15.4816

M3 - Article

VL - 15

SP - 4816

EP - 4822

JO - World Journal of Gastroenterology

JF - World Journal of Gastroenterology

SN - 1007-9327

IS - 38

ER -