Hepatoprotective potential of Fumaria indica Pugsley whole plant extracts, fractions and an isolated alkaloid protopine

A. Rathi, A.K. Srivastava, A. Shirwaikar, A.K. Singh Rawat, S. Mehrotra

Research output: Contribution to journalArticle

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Abstract

The present investigation demonstrates the hepatoprotective potential of 50% ethanolic water extract of whole plant of Fumaria indica and its three fractions viz., hexane, chloroform and butanol against d-galactosamine induced hepatotoxicity in rats. The hepatoprotection was assessed in terms reduction in histological damage, changes in serum enzymes (SGOT, SGPT, ALP) and metabolites bilirubin, reduced glutathione (GSH) and lipid peroxidation (MDA content). Among fractions more than 90% protection was found with butanol fraction in which alkaloid protopine was quantified as highest i.e. about 0.2 mg/g by HPTLC. The isolated protopine in doses of 10-20 mg p.o. also proved equally effective hepatoprotectants as standard drug silymarine (single dose 25 mg p.o.). In general all treatments excluding hexane fraction proved hepatoprotective at par with silymarine (p≤0.01). © 2007 Elsevier GmbH. All rights reserved.
Original languageEnglish
Pages (from-to)470-477
Number of pages8
JournalPhytomedicine
Volume15
Issue number6-7
DOIs
Publication statusPublished - 2008

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Fumaria
Butanols
Plant Extracts
Hexanes
Alkaloids
Galactosamine
Chloroform
Aspartate Aminotransferases
Alanine Transaminase
Bilirubin
Lipid Peroxidation
Glutathione
Water
Enzymes
Serum
Pharmaceutical Preparations
protopine

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Rathi, A. ; Srivastava, A.K. ; Shirwaikar, A. ; Singh Rawat, A.K. ; Mehrotra, S. / Hepatoprotective potential of Fumaria indica Pugsley whole plant extracts, fractions and an isolated alkaloid protopine. In: Phytomedicine. 2008 ; Vol. 15, No. 6-7. pp. 470-477.
@article{0b169aa053ed4b8fa2cf559dcf920831,
title = "Hepatoprotective potential of Fumaria indica Pugsley whole plant extracts, fractions and an isolated alkaloid protopine",
abstract = "The present investigation demonstrates the hepatoprotective potential of 50{\%} ethanolic water extract of whole plant of Fumaria indica and its three fractions viz., hexane, chloroform and butanol against d-galactosamine induced hepatotoxicity in rats. The hepatoprotection was assessed in terms reduction in histological damage, changes in serum enzymes (SGOT, SGPT, ALP) and metabolites bilirubin, reduced glutathione (GSH) and lipid peroxidation (MDA content). Among fractions more than 90{\%} protection was found with butanol fraction in which alkaloid protopine was quantified as highest i.e. about 0.2 mg/g by HPTLC. The isolated protopine in doses of 10-20 mg p.o. also proved equally effective hepatoprotectants as standard drug silymarine (single dose 25 mg p.o.). In general all treatments excluding hexane fraction proved hepatoprotective at par with silymarine (p≤0.01). {\circledC} 2007 Elsevier GmbH. All rights reserved.",
author = "A. Rathi and A.K. Srivastava and A. Shirwaikar and {Singh Rawat}, A.K. and S. Mehrotra",
note = "Cited By :36 Export Date: 10 November 2017 CODEN: PYTOE Correspondence Address: Rathi, A.; Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute, Lucknow, 226 001, India; email: anshurathi@rediffmail.com Chemicals/CAS: alanine aminotransferase, 9000-86-6, 9014-30-6; alkaline phosphatase, 9001-78-9; aspartate aminotransferase, 9000-97-9; bilirubin, 18422-02-1, 635-65-4; glutathione, 70-18-8; protopine, 130-86-9; silymarin, 65666-07-1; Benzophenanthridines; Berberine Alkaloids; Galactosamine, 7535-00-4; Plant Extracts; protopine, 130-86-9 Manufacturers: Sigma, United States References: Abdul-Hussain, S.K., Mehendale, H.M., Studies on the age dependant effect of galactosamine in primary rat hepatocyte cultures (1991) Toxicol. Appl. Pharmacol., 107, pp. 504-513; Anwar, H., Gilani, L., Khalid, H., Janbaz, E., Akhtar, S.M., Selective protective effect of an extract from Fumaria parviflora on paracetamol-induced hepatotoxicity (1996) Gen. Pharmacol., 27, pp. 979-983; Decker, K., Keppler, D., Galactosamine-induced liver injury (1972) Progress in Liver Disease, pp. 183-199. , Popper H., and Schaffner F. (Eds), Grune and Stratton, New York; Decker, K., Keppler, D., Galactosamine hepatitis: key role of the nucleotide deficiency period in the pathogenesis of cell injury and cell death (1974) Rev. Physiol.. Biochem. Pharmacol., 71, pp. 78-105; Deng, M., Song, X.Y., Jiang, B., Effects of protopine on proliferation of cultured rabbit aortic vascular smooth muscle cells (2001) Chin. Pharmacol. Bull., 17, pp. 306-309; Ellman, G.L., Tissue sulphydryl groups (1959) Arch. Biochem. Biophys., 82, p. 70; Gerbes, A.L., {\'A}vila, M.A., Caselmann, W.H., Liver injury and liver protection: mechanisms and novel treatment strategies (2006) Liver Int., 26, pp. 902-903; Keppler, D.O., Rudigier, J.F., Bischoff, E., Decker, K.F., The trapping of uridine phosphates by d-galactosamine, d-glucosamine, and 2-deoxy-d-galactose. A study on the mechanism of galactosamine hepatitis (1970) Eur. J. Biochem., 17, pp. 246-253; King, E.J., Armstrong, A.R., Calcium, magnesium phosphorus and phosphatase (1980) Practical Clinical Biochemistry, 1, pp. 850-852. , Varley B., Gowenlock A.H., and Bell M. (Eds), Heinemann, London; Ko, F.N., Wu, T.S., Lu, S.U., Wu, Y.C., Huang, T.F., Teng, C.M., Ca2+ channel blockade in rat thoracic aorta by protopine isolated from corydalis tubers (1992) Jpn. J. Pharmacol., 58, pp. 1-9; Kurma, S.R., Mishra, S.H., Hepatoprotective activity of the whole plant of Fumaria indica (1997) Indian J. Pharm. Sci., 59, pp. 165-170; Lin, C., Yen, M., Lo, T., Lin, J., Evaluation of hepatoprotective and antioxidant activity of Boehmeria nivea Var. nivea and B. nivea Var. tenacissima (1998) J. Ethnopharmacol., 60, pp. 9-17; Liu, J., Xiao, P.G., Recent advances in the study of antioxidative effects of Chinese medicinal plants (1994) Phytother. Res., 8, pp. 445-451; Malloy, H.T., Evelyn, K.A., The determination of bilirubin with the photometric colorimeter (1937) J. Biol. Chem., 119, pp. 481-490; Ohkawa, H., Ohishi, N., Yagi, K., Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction (1979) Anal. Biochem., 95, pp. 351-358; Ohta, Y., Sasaki, E., Nishida, K., Kongo, M., Hayashi, T., Nagata, M., Ishiguro, L., Contribution of the antilipid peroxidative action of Dai-saiko-to extract to its preventive effect on carbon tetrachloride-induced acute liver injury in rats (1998) Phytother. Res., 12, pp. 5-8; Orhan, D.D., Orhana, N., Ergun, E., Ergun, F., Hepatoprotective effect of Vitis vinifera L. leaves on carbon tetrachloride-induced acute liver damage in rats (2007) J. Ethnopharmacol., 112, pp. 145-151; Pandey, V.B., Dasguta, B., Bhattacharya, S.K., Lal, R., Das, P.K., Chemistry and pharmacology of the major alkaloid of Fumaria indica (Haussk) Pugsley (1971) Curr. Sci., 17, pp. 455-457; Rao, K.S., Mishra, S.H., Hepatoprotective activities of whole plants of Fumaria indica (1997) Indian Drugs, 59, p. 165; Rao, K.S., Mishra, S.H., Antihepatotoxic activity of monomethyl fumarate isolated from Fumaria indica (1998) J. Ethnopharmacol., 60, pp. 207-213; Reitman, S., Frankel, S., A colourimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases (1957) Am. J Clin. Pathol., 28, pp. 56-63; Song, L.S., Ren, G.J., Chen, Z.L., Chen, Z.H., Zhou, Z.N., Cheng, H.P., Electrophysiological effects of protopine in cardiac myocytes: inhibition of multiple cation channel currents (2000) Br. J. Pharmacol., 129, pp. 893-900; Song, X.Y., Jiang, B., Wang, Z.Z., Chen, W.N., LI, N., Gao, E., Protective effect of protopine on ischemia-reperfusion myocardium in rats (2000) Chin. J. Pathophysiol., 16, p. 989; Venkateswaran, S., Pari, L., Viswanathan, P., Anti peroxidation effect of Livex, an herbal formulation against erythromycin estolate induced lipid peroxidation in rats (1998) Phytother. Res., 12, pp. 465-471; Wolf, P.L., Biochemical diagnosis of liver diseases (1999) Indian J. Clin. Biochem., 14, pp. 59-90; Yamaguchi, M., Miyashita, Y., Kumagai, Y., Kojo, S., Change in liver and plasma ceramides during d-galactosamine induced acute hepatic injury by LC-MS/MS (2004) Bioorg. Med. Chem. Lett., 14, pp. 4061-4064; Zimmerman, M., Ethical guidelines for investigations of experimental pain in conscious animals (1983) Pain, 16, pp. 109-110",
year = "2008",
doi = "10.1016/j.phymed.2007.11.010",
language = "English",
volume = "15",
pages = "470--477",
journal = "Phytomedicine",
issn = "0944-7113",
publisher = "Urban und Fischer Verlag Jena",
number = "6-7",

}

Hepatoprotective potential of Fumaria indica Pugsley whole plant extracts, fractions and an isolated alkaloid protopine. / Rathi, A.; Srivastava, A.K.; Shirwaikar, A.; Singh Rawat, A.K.; Mehrotra, S.

In: Phytomedicine, Vol. 15, No. 6-7, 2008, p. 470-477.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Hepatoprotective potential of Fumaria indica Pugsley whole plant extracts, fractions and an isolated alkaloid protopine

AU - Rathi, A.

AU - Srivastava, A.K.

AU - Shirwaikar, A.

AU - Singh Rawat, A.K.

AU - Mehrotra, S.

N1 - Cited By :36 Export Date: 10 November 2017 CODEN: PYTOE Correspondence Address: Rathi, A.; Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute, Lucknow, 226 001, India; email: anshurathi@rediffmail.com Chemicals/CAS: alanine aminotransferase, 9000-86-6, 9014-30-6; alkaline phosphatase, 9001-78-9; aspartate aminotransferase, 9000-97-9; bilirubin, 18422-02-1, 635-65-4; glutathione, 70-18-8; protopine, 130-86-9; silymarin, 65666-07-1; Benzophenanthridines; Berberine Alkaloids; Galactosamine, 7535-00-4; Plant Extracts; protopine, 130-86-9 Manufacturers: Sigma, United States References: Abdul-Hussain, S.K., Mehendale, H.M., Studies on the age dependant effect of galactosamine in primary rat hepatocyte cultures (1991) Toxicol. Appl. Pharmacol., 107, pp. 504-513; Anwar, H., Gilani, L., Khalid, H., Janbaz, E., Akhtar, S.M., Selective protective effect of an extract from Fumaria parviflora on paracetamol-induced hepatotoxicity (1996) Gen. Pharmacol., 27, pp. 979-983; Decker, K., Keppler, D., Galactosamine-induced liver injury (1972) Progress in Liver Disease, pp. 183-199. , Popper H., and Schaffner F. (Eds), Grune and Stratton, New York; Decker, K., Keppler, D., Galactosamine hepatitis: key role of the nucleotide deficiency period in the pathogenesis of cell injury and cell death (1974) Rev. Physiol.. Biochem. Pharmacol., 71, pp. 78-105; Deng, M., Song, X.Y., Jiang, B., Effects of protopine on proliferation of cultured rabbit aortic vascular smooth muscle cells (2001) Chin. Pharmacol. Bull., 17, pp. 306-309; Ellman, G.L., Tissue sulphydryl groups (1959) Arch. Biochem. Biophys., 82, p. 70; Gerbes, A.L., Ávila, M.A., Caselmann, W.H., Liver injury and liver protection: mechanisms and novel treatment strategies (2006) Liver Int., 26, pp. 902-903; Keppler, D.O., Rudigier, J.F., Bischoff, E., Decker, K.F., The trapping of uridine phosphates by d-galactosamine, d-glucosamine, and 2-deoxy-d-galactose. A study on the mechanism of galactosamine hepatitis (1970) Eur. J. Biochem., 17, pp. 246-253; King, E.J., Armstrong, A.R., Calcium, magnesium phosphorus and phosphatase (1980) Practical Clinical Biochemistry, 1, pp. 850-852. , Varley B., Gowenlock A.H., and Bell M. (Eds), Heinemann, London; Ko, F.N., Wu, T.S., Lu, S.U., Wu, Y.C., Huang, T.F., Teng, C.M., Ca2+ channel blockade in rat thoracic aorta by protopine isolated from corydalis tubers (1992) Jpn. J. Pharmacol., 58, pp. 1-9; Kurma, S.R., Mishra, S.H., Hepatoprotective activity of the whole plant of Fumaria indica (1997) Indian J. Pharm. Sci., 59, pp. 165-170; Lin, C., Yen, M., Lo, T., Lin, J., Evaluation of hepatoprotective and antioxidant activity of Boehmeria nivea Var. nivea and B. nivea Var. tenacissima (1998) J. Ethnopharmacol., 60, pp. 9-17; Liu, J., Xiao, P.G., Recent advances in the study of antioxidative effects of Chinese medicinal plants (1994) Phytother. Res., 8, pp. 445-451; Malloy, H.T., Evelyn, K.A., The determination of bilirubin with the photometric colorimeter (1937) J. Biol. Chem., 119, pp. 481-490; Ohkawa, H., Ohishi, N., Yagi, K., Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction (1979) Anal. Biochem., 95, pp. 351-358; Ohta, Y., Sasaki, E., Nishida, K., Kongo, M., Hayashi, T., Nagata, M., Ishiguro, L., Contribution of the antilipid peroxidative action of Dai-saiko-to extract to its preventive effect on carbon tetrachloride-induced acute liver injury in rats (1998) Phytother. Res., 12, pp. 5-8; Orhan, D.D., Orhana, N., Ergun, E., Ergun, F., Hepatoprotective effect of Vitis vinifera L. leaves on carbon tetrachloride-induced acute liver damage in rats (2007) J. Ethnopharmacol., 112, pp. 145-151; Pandey, V.B., Dasguta, B., Bhattacharya, S.K., Lal, R., Das, P.K., Chemistry and pharmacology of the major alkaloid of Fumaria indica (Haussk) Pugsley (1971) Curr. Sci., 17, pp. 455-457; Rao, K.S., Mishra, S.H., Hepatoprotective activities of whole plants of Fumaria indica (1997) Indian Drugs, 59, p. 165; Rao, K.S., Mishra, S.H., Antihepatotoxic activity of monomethyl fumarate isolated from Fumaria indica (1998) J. Ethnopharmacol., 60, pp. 207-213; Reitman, S., Frankel, S., A colourimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases (1957) Am. J Clin. Pathol., 28, pp. 56-63; Song, L.S., Ren, G.J., Chen, Z.L., Chen, Z.H., Zhou, Z.N., Cheng, H.P., Electrophysiological effects of protopine in cardiac myocytes: inhibition of multiple cation channel currents (2000) Br. J. Pharmacol., 129, pp. 893-900; Song, X.Y., Jiang, B., Wang, Z.Z., Chen, W.N., LI, N., Gao, E., Protective effect of protopine on ischemia-reperfusion myocardium in rats (2000) Chin. J. Pathophysiol., 16, p. 989; Venkateswaran, S., Pari, L., Viswanathan, P., Anti peroxidation effect of Livex, an herbal formulation against erythromycin estolate induced lipid peroxidation in rats (1998) Phytother. Res., 12, pp. 465-471; Wolf, P.L., Biochemical diagnosis of liver diseases (1999) Indian J. Clin. Biochem., 14, pp. 59-90; Yamaguchi, M., Miyashita, Y., Kumagai, Y., Kojo, S., Change in liver and plasma ceramides during d-galactosamine induced acute hepatic injury by LC-MS/MS (2004) Bioorg. Med. Chem. Lett., 14, pp. 4061-4064; Zimmerman, M., Ethical guidelines for investigations of experimental pain in conscious animals (1983) Pain, 16, pp. 109-110

PY - 2008

Y1 - 2008

N2 - The present investigation demonstrates the hepatoprotective potential of 50% ethanolic water extract of whole plant of Fumaria indica and its three fractions viz., hexane, chloroform and butanol against d-galactosamine induced hepatotoxicity in rats. The hepatoprotection was assessed in terms reduction in histological damage, changes in serum enzymes (SGOT, SGPT, ALP) and metabolites bilirubin, reduced glutathione (GSH) and lipid peroxidation (MDA content). Among fractions more than 90% protection was found with butanol fraction in which alkaloid protopine was quantified as highest i.e. about 0.2 mg/g by HPTLC. The isolated protopine in doses of 10-20 mg p.o. also proved equally effective hepatoprotectants as standard drug silymarine (single dose 25 mg p.o.). In general all treatments excluding hexane fraction proved hepatoprotective at par with silymarine (p≤0.01). © 2007 Elsevier GmbH. All rights reserved.

AB - The present investigation demonstrates the hepatoprotective potential of 50% ethanolic water extract of whole plant of Fumaria indica and its three fractions viz., hexane, chloroform and butanol against d-galactosamine induced hepatotoxicity in rats. The hepatoprotection was assessed in terms reduction in histological damage, changes in serum enzymes (SGOT, SGPT, ALP) and metabolites bilirubin, reduced glutathione (GSH) and lipid peroxidation (MDA content). Among fractions more than 90% protection was found with butanol fraction in which alkaloid protopine was quantified as highest i.e. about 0.2 mg/g by HPTLC. The isolated protopine in doses of 10-20 mg p.o. also proved equally effective hepatoprotectants as standard drug silymarine (single dose 25 mg p.o.). In general all treatments excluding hexane fraction proved hepatoprotective at par with silymarine (p≤0.01). © 2007 Elsevier GmbH. All rights reserved.

U2 - 10.1016/j.phymed.2007.11.010

DO - 10.1016/j.phymed.2007.11.010

M3 - Article

VL - 15

SP - 470

EP - 477

JO - Phytomedicine

JF - Phytomedicine

SN - 0944-7113

IS - 6-7

ER -