HER2 and helicobacter pylori status in resected gastric cancers

A pathological study of a gastroenterological issue

Saraswathy Sreeram, Shrijeet Chakraborti, Ramdas Naik, Debarshi Saha, Yeshwanter Radhakrishnan, Hanaganahalli Basavaiah Sridevi, Sharada Rai, Chaithra Gowthuvalli Venkataramana

Research output: Contribution to journalArticle

Abstract

Introduction: Human epidermal growth factor receptor 2 (HER2)/neu is a critical target for gastric carcinoma treatment utilizing trastuzumab. Helicobacter pylori is a well known causative agent of gastric carcinoma. Aim: To study association of HER2/neu expression with the presence of H. pylori infection in resected carcinoma stomach patients. Materials and Methods: A cross-sectional study of 85 gastrectomies received in the department from January 2010 to September 2014 was done. HER2/neu was studied using Immunohistochemistry (IHC) and Giemsa stain was used to detect presence of H. pylori. Chi-square test and Fisher’s exact test were used, to test the correlation between the various parameters. A p-value <0.05 was considered significant. Results: Our study population included 67 (78.8%) males, and 18 (21.2%) females, ranging from 22 to 84 years, mean 57.68±12.12 years. HER-2 expression, graded from 0 to 3± was correlated with location, histologic type, grade, local invasion, metastasis to lymph nodes, TNM tumour staging and H. pylori infection, graded from 0 to 3+ using Giemsa stain. HER2/neu 3+ was observed in intestinal type of gastric cancer (5/55, 9%) only. Scores 2+ and 3+ were more common in H. pylori-negative patients (5/26, 19.2%) than H. pylori-positive patients (4/59, 6.8%) (p=0.02). TNM stage, extent of local invasion and lymph node metastasis in intestinal gastric carcinomas correlated significantly with HER2/neu expression. H. pylori was present in 59 (69.4%) and absent in 26 (30.6%). Conclusion: H. pylori-negative gastric cancer showed significant immunophenotypic HER2/neu overexpression i.e., H. pylori might protect against HER2 overexpression that correlated significantly with higher TNM stages of intestinal-type gastric cancer. In contrast, H. pylori infection correlated significantly with Lymph-Vascular Invasion (LVI) but was pN1/2+, thereby diminishing prognostic importance. H. pylori induced intestinal metaplasia was not significantly associated with intestinal-type gastric cancer.

Original languageEnglish
Pages (from-to)EC01-EC05
JournalJournal of Clinical and Diagnostic Research
Volume11
Issue number10
DOIs
Publication statusPublished - 01-10-2017

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Helicobacter pylori
Stomach Neoplasms
Azure Stains
Intestinal Neoplasms
Helicobacter Infections
Carcinoma
Stomach
Neoplasm Staging
Gastrointestinal Agents
human ERBB2 protein
Lymph Nodes
Neoplasm Metastasis
Tumors
Metaplasia
Lymph
Chi-Square Distribution
Gastrectomy
Blood Vessels
Cross-Sectional Studies
Immunohistochemistry

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry

Cite this

Sreeram, Saraswathy ; Chakraborti, Shrijeet ; Naik, Ramdas ; Saha, Debarshi ; Radhakrishnan, Yeshwanter ; Sridevi, Hanaganahalli Basavaiah ; Rai, Sharada ; Venkataramana, Chaithra Gowthuvalli. / HER2 and helicobacter pylori status in resected gastric cancers : A pathological study of a gastroenterological issue. In: Journal of Clinical and Diagnostic Research. 2017 ; Vol. 11, No. 10. pp. EC01-EC05.
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abstract = "Introduction: Human epidermal growth factor receptor 2 (HER2)/neu is a critical target for gastric carcinoma treatment utilizing trastuzumab. Helicobacter pylori is a well known causative agent of gastric carcinoma. Aim: To study association of HER2/neu expression with the presence of H. pylori infection in resected carcinoma stomach patients. Materials and Methods: A cross-sectional study of 85 gastrectomies received in the department from January 2010 to September 2014 was done. HER2/neu was studied using Immunohistochemistry (IHC) and Giemsa stain was used to detect presence of H. pylori. Chi-square test and Fisher’s exact test were used, to test the correlation between the various parameters. A p-value <0.05 was considered significant. Results: Our study population included 67 (78.8{\%}) males, and 18 (21.2{\%}) females, ranging from 22 to 84 years, mean 57.68±12.12 years. HER-2 expression, graded from 0 to 3± was correlated with location, histologic type, grade, local invasion, metastasis to lymph nodes, TNM tumour staging and H. pylori infection, graded from 0 to 3+ using Giemsa stain. HER2/neu 3+ was observed in intestinal type of gastric cancer (5/55, 9{\%}) only. Scores 2+ and 3+ were more common in H. pylori-negative patients (5/26, 19.2{\%}) than H. pylori-positive patients (4/59, 6.8{\%}) (p=0.02). TNM stage, extent of local invasion and lymph node metastasis in intestinal gastric carcinomas correlated significantly with HER2/neu expression. H. pylori was present in 59 (69.4{\%}) and absent in 26 (30.6{\%}). Conclusion: H. pylori-negative gastric cancer showed significant immunophenotypic HER2/neu overexpression i.e., H. pylori might protect against HER2 overexpression that correlated significantly with higher TNM stages of intestinal-type gastric cancer. In contrast, H. pylori infection correlated significantly with Lymph-Vascular Invasion (LVI) but was pN1/2+, thereby diminishing prognostic importance. H. pylori induced intestinal metaplasia was not significantly associated with intestinal-type gastric cancer.",
author = "Saraswathy Sreeram and Shrijeet Chakraborti and Ramdas Naik and Debarshi Saha and Yeshwanter Radhakrishnan and Sridevi, {Hanaganahalli Basavaiah} and Sharada Rai and Venkataramana, {Chaithra Gowthuvalli}",
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HER2 and helicobacter pylori status in resected gastric cancers : A pathological study of a gastroenterological issue. / Sreeram, Saraswathy; Chakraborti, Shrijeet; Naik, Ramdas; Saha, Debarshi; Radhakrishnan, Yeshwanter; Sridevi, Hanaganahalli Basavaiah; Rai, Sharada; Venkataramana, Chaithra Gowthuvalli.

In: Journal of Clinical and Diagnostic Research, Vol. 11, No. 10, 01.10.2017, p. EC01-EC05.

Research output: Contribution to journalArticle

TY - JOUR

T1 - HER2 and helicobacter pylori status in resected gastric cancers

T2 - A pathological study of a gastroenterological issue

AU - Sreeram, Saraswathy

AU - Chakraborti, Shrijeet

AU - Naik, Ramdas

AU - Saha, Debarshi

AU - Radhakrishnan, Yeshwanter

AU - Sridevi, Hanaganahalli Basavaiah

AU - Rai, Sharada

AU - Venkataramana, Chaithra Gowthuvalli

PY - 2017/10/1

Y1 - 2017/10/1

N2 - Introduction: Human epidermal growth factor receptor 2 (HER2)/neu is a critical target for gastric carcinoma treatment utilizing trastuzumab. Helicobacter pylori is a well known causative agent of gastric carcinoma. Aim: To study association of HER2/neu expression with the presence of H. pylori infection in resected carcinoma stomach patients. Materials and Methods: A cross-sectional study of 85 gastrectomies received in the department from January 2010 to September 2014 was done. HER2/neu was studied using Immunohistochemistry (IHC) and Giemsa stain was used to detect presence of H. pylori. Chi-square test and Fisher’s exact test were used, to test the correlation between the various parameters. A p-value <0.05 was considered significant. Results: Our study population included 67 (78.8%) males, and 18 (21.2%) females, ranging from 22 to 84 years, mean 57.68±12.12 years. HER-2 expression, graded from 0 to 3± was correlated with location, histologic type, grade, local invasion, metastasis to lymph nodes, TNM tumour staging and H. pylori infection, graded from 0 to 3+ using Giemsa stain. HER2/neu 3+ was observed in intestinal type of gastric cancer (5/55, 9%) only. Scores 2+ and 3+ were more common in H. pylori-negative patients (5/26, 19.2%) than H. pylori-positive patients (4/59, 6.8%) (p=0.02). TNM stage, extent of local invasion and lymph node metastasis in intestinal gastric carcinomas correlated significantly with HER2/neu expression. H. pylori was present in 59 (69.4%) and absent in 26 (30.6%). Conclusion: H. pylori-negative gastric cancer showed significant immunophenotypic HER2/neu overexpression i.e., H. pylori might protect against HER2 overexpression that correlated significantly with higher TNM stages of intestinal-type gastric cancer. In contrast, H. pylori infection correlated significantly with Lymph-Vascular Invasion (LVI) but was pN1/2+, thereby diminishing prognostic importance. H. pylori induced intestinal metaplasia was not significantly associated with intestinal-type gastric cancer.

AB - Introduction: Human epidermal growth factor receptor 2 (HER2)/neu is a critical target for gastric carcinoma treatment utilizing trastuzumab. Helicobacter pylori is a well known causative agent of gastric carcinoma. Aim: To study association of HER2/neu expression with the presence of H. pylori infection in resected carcinoma stomach patients. Materials and Methods: A cross-sectional study of 85 gastrectomies received in the department from January 2010 to September 2014 was done. HER2/neu was studied using Immunohistochemistry (IHC) and Giemsa stain was used to detect presence of H. pylori. Chi-square test and Fisher’s exact test were used, to test the correlation between the various parameters. A p-value <0.05 was considered significant. Results: Our study population included 67 (78.8%) males, and 18 (21.2%) females, ranging from 22 to 84 years, mean 57.68±12.12 years. HER-2 expression, graded from 0 to 3± was correlated with location, histologic type, grade, local invasion, metastasis to lymph nodes, TNM tumour staging and H. pylori infection, graded from 0 to 3+ using Giemsa stain. HER2/neu 3+ was observed in intestinal type of gastric cancer (5/55, 9%) only. Scores 2+ and 3+ were more common in H. pylori-negative patients (5/26, 19.2%) than H. pylori-positive patients (4/59, 6.8%) (p=0.02). TNM stage, extent of local invasion and lymph node metastasis in intestinal gastric carcinomas correlated significantly with HER2/neu expression. H. pylori was present in 59 (69.4%) and absent in 26 (30.6%). Conclusion: H. pylori-negative gastric cancer showed significant immunophenotypic HER2/neu overexpression i.e., H. pylori might protect against HER2 overexpression that correlated significantly with higher TNM stages of intestinal-type gastric cancer. In contrast, H. pylori infection correlated significantly with Lymph-Vascular Invasion (LVI) but was pN1/2+, thereby diminishing prognostic importance. H. pylori induced intestinal metaplasia was not significantly associated with intestinal-type gastric cancer.

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