High performance liquid chromatographic determination of carboplatin in injections

G. P. Agrawal, G. Shenoy, A. Ghosh, K. Bhat, L. Prabhu

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The present study describes the development and validation of a stability indicating reverse phase HPLC (RP-HPLC) method for the analysis of carboplatin, an anticancer drug, in injection dosage forms. The proposed RP-HPLC method utilizes Inertsil amino, 250 mm × 4.6 mm i.d., 5 μ column (at ambient temperature), isocratic run (using acetonitrile and water as mobile phase), at a flow rate of 2.0 mL min-1 and UV detection at 230 nm for analysis of carboplatin. The reported method is linear. Carboplatin was exposed to acidic, alkaline oxidative, thermal and photolytic stress conditions-and the stressed samples were analyzed by the proposed method. Chromatographic peak purity results indicated the absence of co-eluting peaks with the drug peak, which demonstrated the specificity of assay method fo estimation of carboplatin in presence of degradation products. The proposed method can be used for routine analysis stability testing o carboplatin in quality control laboratories.

Original languageEnglish
Pages (from-to)5095-5098
Number of pages4
JournalAsian Journal of Chemistry
Volume23
Issue number11
Publication statusPublished - 06-10-2011

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

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