High performance liquid chromatographic fluorescence detection method for the quantification of rivastigmine in rat plasma and brain

Application to preclinical pharmacokinetic studies in rats

K. Arumugam, M. R. Chamallamudi, S. R. Mallayasamy, R. Mullangi, S. Ganesan, L. Jamadar, A. Ranjithkumar, N. Udupa

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A highly sensitive and selective high performance liquid chromatographic fluorescence detection method has been developed and validated for the quantification of rivastigmine in rat plasma and brain. Protein precipitation and one-step liquid-liquid extraction techniques were utilized for the extraction of RSM from brain and plasma, respectively, along with an internal standard. The chromatographic separation was achieved with a column inertsil ODS-3V and a mobile phase consisting of ammonium acetate buffer (20 mM, pH 4.5) and acetonitrile (76:24, v/v) delivered at a flow rate of 1 ml/min. The lower limit of quantitation for the developed method was 10 ng/mL for both matrices. The method was found to be accurate and reproducible and was successfully used to quantify levels of RSM in plasma and brain following intravenous administration of RSM in rats.

Original languageEnglish
Pages (from-to)315-321
Number of pages7
JournalJournal of Young Pharmacists
Volume3
Issue number4
DOIs
Publication statusPublished - 2011

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Rivastigmine
Pharmacokinetics
Fluorescence
Brain
Liquid-Liquid Extraction
Intravenous Administration
Buffers
Proteins

All Science Journal Classification (ASJC) codes

  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

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title = "High performance liquid chromatographic fluorescence detection method for the quantification of rivastigmine in rat plasma and brain: Application to preclinical pharmacokinetic studies in rats",
abstract = "A highly sensitive and selective high performance liquid chromatographic fluorescence detection method has been developed and validated for the quantification of rivastigmine in rat plasma and brain. Protein precipitation and one-step liquid-liquid extraction techniques were utilized for the extraction of RSM from brain and plasma, respectively, along with an internal standard. The chromatographic separation was achieved with a column inertsil ODS-3V and a mobile phase consisting of ammonium acetate buffer (20 mM, pH 4.5) and acetonitrile (76:24, v/v) delivered at a flow rate of 1 ml/min. The lower limit of quantitation for the developed method was 10 ng/mL for both matrices. The method was found to be accurate and reproducible and was successfully used to quantify levels of RSM in plasma and brain following intravenous administration of RSM in rats.",
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T2 - Application to preclinical pharmacokinetic studies in rats

AU - Arumugam, K.

AU - Chamallamudi, M. R.

AU - Mallayasamy, S. R.

AU - Mullangi, R.

AU - Ganesan, S.

AU - Jamadar, L.

AU - Ranjithkumar, A.

AU - Udupa, N.

PY - 2011

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