High performance liquid chromatographic method for determination of clobetasol in rat plasma and its application to skin penetration

Prashant B. Musmade, Praful B. Deshpande, Swapnil J. Dengle, Kranti B. Talole, Shriram M. Pathak, M. N.S. Adiga, Krishnamurthy Bhat, Nayanabhirama Udupa, K. Laxminarayana Bairy

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A simple and sensitive high performance liquid chromatography (HPLC) method was developed for quantification of clobetasol (CLB) in rat plasma. Aripiprazole was used as an internal standard (IS). The present method uses protein precipitation with acetonitrile, followed by the liquid-liquid extraction with methyl tertiary butyl ether for extraction of the CLB from the matrix. Separation was carried out using Variance C18 (250.0 × 4.6 mm, 5 μm particle size) column and the effluent was monitored by an ultra violet (UV) detector at 240 nm. The mobile phase used was acetonitrile: phosphate buffer (pH 7.0; 25.0mM containing 0.2 %v/v triethylamine) (65: 35 % v/v) at a flow rate of 1.0 ml/min. The present method was validated as per the United States Food and Drug Administration (USFDA) guideline. This method was linear over the range of 25.0-1000.0 ng/ml with regression coefficient greater than 0.99. The mean recovery of CLB and IS were 70.8±1.9 and 83.32±1.55 % respectively. The method was found to be precise, accurate and specific. The developed HPLC method was applied for the estimation of CLB in plasma after topical application of various zinc salts in rats to study the penetration of CLB through rat skin.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalJournal of Bioanalysis and Biomedicine
Volume2
Issue number1
DOIs
Publication statusPublished - 02-2010

Fingerprint

Clobetasol
Skin
High Pressure Liquid Chromatography
Liquid-Liquid Extraction
United States Food and Drug Administration
Particle Size
Ether
Zinc
Buffers
Salts
Phosphates
Guidelines

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science

Cite this

Musmade, Prashant B. ; Deshpande, Praful B. ; Dengle, Swapnil J. ; Talole, Kranti B. ; Pathak, Shriram M. ; Adiga, M. N.S. ; Bhat, Krishnamurthy ; Udupa, Nayanabhirama ; Laxminarayana Bairy, K. / High performance liquid chromatographic method for determination of clobetasol in rat plasma and its application to skin penetration. In: Journal of Bioanalysis and Biomedicine. 2010 ; Vol. 2, No. 1. pp. 1-7.
@article{b06c87f27be94f19ad08387bcfecc7a0,
title = "High performance liquid chromatographic method for determination of clobetasol in rat plasma and its application to skin penetration",
abstract = "A simple and sensitive high performance liquid chromatography (HPLC) method was developed for quantification of clobetasol (CLB) in rat plasma. Aripiprazole was used as an internal standard (IS). The present method uses protein precipitation with acetonitrile, followed by the liquid-liquid extraction with methyl tertiary butyl ether for extraction of the CLB from the matrix. Separation was carried out using Variance C18 (250.0 × 4.6 mm, 5 μm particle size) column and the effluent was monitored by an ultra violet (UV) detector at 240 nm. The mobile phase used was acetonitrile: phosphate buffer (pH 7.0; 25.0mM containing 0.2 {\%}v/v triethylamine) (65: 35 {\%} v/v) at a flow rate of 1.0 ml/min. The present method was validated as per the United States Food and Drug Administration (USFDA) guideline. This method was linear over the range of 25.0-1000.0 ng/ml with regression coefficient greater than 0.99. The mean recovery of CLB and IS were 70.8±1.9 and 83.32±1.55 {\%} respectively. The method was found to be precise, accurate and specific. The developed HPLC method was applied for the estimation of CLB in plasma after topical application of various zinc salts in rats to study the penetration of CLB through rat skin.",
author = "Musmade, {Prashant B.} and Deshpande, {Praful B.} and Dengle, {Swapnil J.} and Talole, {Kranti B.} and Pathak, {Shriram M.} and Adiga, {M. N.S.} and Krishnamurthy Bhat and Nayanabhirama Udupa and {Laxminarayana Bairy}, K.",
year = "2010",
month = "2",
doi = "10.4172/1948-593X.1000013",
language = "English",
volume = "2",
pages = "1--7",
journal = "Journal of Bioanalysis and Biomedicine",
issn = "1948-593X",
publisher = "OMICS Publishing Group",
number = "1",

}

High performance liquid chromatographic method for determination of clobetasol in rat plasma and its application to skin penetration. / Musmade, Prashant B.; Deshpande, Praful B.; Dengle, Swapnil J.; Talole, Kranti B.; Pathak, Shriram M.; Adiga, M. N.S.; Bhat, Krishnamurthy; Udupa, Nayanabhirama; Laxminarayana Bairy, K.

In: Journal of Bioanalysis and Biomedicine, Vol. 2, No. 1, 02.2010, p. 1-7.

Research output: Contribution to journalArticle

TY - JOUR

T1 - High performance liquid chromatographic method for determination of clobetasol in rat plasma and its application to skin penetration

AU - Musmade, Prashant B.

AU - Deshpande, Praful B.

AU - Dengle, Swapnil J.

AU - Talole, Kranti B.

AU - Pathak, Shriram M.

AU - Adiga, M. N.S.

AU - Bhat, Krishnamurthy

AU - Udupa, Nayanabhirama

AU - Laxminarayana Bairy, K.

PY - 2010/2

Y1 - 2010/2

N2 - A simple and sensitive high performance liquid chromatography (HPLC) method was developed for quantification of clobetasol (CLB) in rat plasma. Aripiprazole was used as an internal standard (IS). The present method uses protein precipitation with acetonitrile, followed by the liquid-liquid extraction with methyl tertiary butyl ether for extraction of the CLB from the matrix. Separation was carried out using Variance C18 (250.0 × 4.6 mm, 5 μm particle size) column and the effluent was monitored by an ultra violet (UV) detector at 240 nm. The mobile phase used was acetonitrile: phosphate buffer (pH 7.0; 25.0mM containing 0.2 %v/v triethylamine) (65: 35 % v/v) at a flow rate of 1.0 ml/min. The present method was validated as per the United States Food and Drug Administration (USFDA) guideline. This method was linear over the range of 25.0-1000.0 ng/ml with regression coefficient greater than 0.99. The mean recovery of CLB and IS were 70.8±1.9 and 83.32±1.55 % respectively. The method was found to be precise, accurate and specific. The developed HPLC method was applied for the estimation of CLB in plasma after topical application of various zinc salts in rats to study the penetration of CLB through rat skin.

AB - A simple and sensitive high performance liquid chromatography (HPLC) method was developed for quantification of clobetasol (CLB) in rat plasma. Aripiprazole was used as an internal standard (IS). The present method uses protein precipitation with acetonitrile, followed by the liquid-liquid extraction with methyl tertiary butyl ether for extraction of the CLB from the matrix. Separation was carried out using Variance C18 (250.0 × 4.6 mm, 5 μm particle size) column and the effluent was monitored by an ultra violet (UV) detector at 240 nm. The mobile phase used was acetonitrile: phosphate buffer (pH 7.0; 25.0mM containing 0.2 %v/v triethylamine) (65: 35 % v/v) at a flow rate of 1.0 ml/min. The present method was validated as per the United States Food and Drug Administration (USFDA) guideline. This method was linear over the range of 25.0-1000.0 ng/ml with regression coefficient greater than 0.99. The mean recovery of CLB and IS were 70.8±1.9 and 83.32±1.55 % respectively. The method was found to be precise, accurate and specific. The developed HPLC method was applied for the estimation of CLB in plasma after topical application of various zinc salts in rats to study the penetration of CLB through rat skin.

UR - http://www.scopus.com/inward/record.url?scp=79952607422&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79952607422&partnerID=8YFLogxK

U2 - 10.4172/1948-593X.1000013

DO - 10.4172/1948-593X.1000013

M3 - Article

AN - SCOPUS:79952607422

VL - 2

SP - 1

EP - 7

JO - Journal of Bioanalysis and Biomedicine

JF - Journal of Bioanalysis and Biomedicine

SN - 1948-593X

IS - 1

ER -