High-performance liquid chromatography and pharmacokinetics of aceclofenac in rats

P. Musmade, G. Subramanian, K.K. Srinivasan

Research output: Contribution to journalArticle

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Abstract

A simple and sensitive high-performance liquid chromatographic (HPLC) method was developed for quantification of aceclofenac in rat plasma. Ibuprofen was used as an internal standard (IS). The present method used protein precipitation for extraction of aceclofenac from rat plasma. Separation was carried out on reversed-phase C18 column (250 mm × 4.6 mm, 5 μ) and the column effluent was monitored by UV detector at 282 nm. The mobile phase used was methanol-triethylamine (pH 7.0; 0.3% v/v in Milli-Q water) (60:40%, v/v) at a flow rate of 1.0 mL min-1. This method was linear over the range of 50.0-3500.0 ng mL-1 with regression coefficient greater than 0.99. The mean recovery of aceclofenac and IS were 84.62 ± 3.23 and 89.19 ± 1.57%, respectively and the method was found to be precise, accurate, and specific during the study. The method was successfully applied for pharmacokinetic study of aceclofenac in rats. © 2006 Elsevier B.V. All rights reserved.
Original languageEnglish
Pages (from-to)103-109
Number of pages7
JournalAnalytica Chimica Acta
Volume585
Issue number1
DOIs
Publication statusPublished - 2007

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Pharmacokinetics
High performance liquid chromatography
Rats
liquid chromatography
High Pressure Liquid Chromatography
Ultraviolet detectors
Plasmas
Ibuprofen
plasma
Methanol
Effluents
Flow rate
methanol
Recovery
aceclofenac
method
pharmacokinetics
Water
effluent
Liquids

Cite this

Musmade, P. ; Subramanian, G. ; Srinivasan, K.K. / High-performance liquid chromatography and pharmacokinetics of aceclofenac in rats. In: Analytica Chimica Acta. 2007 ; Vol. 585, No. 1. pp. 103-109.
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abstract = "A simple and sensitive high-performance liquid chromatographic (HPLC) method was developed for quantification of aceclofenac in rat plasma. Ibuprofen was used as an internal standard (IS). The present method used protein precipitation for extraction of aceclofenac from rat plasma. Separation was carried out on reversed-phase C18 column (250 mm × 4.6 mm, 5 μ) and the column effluent was monitored by UV detector at 282 nm. The mobile phase used was methanol-triethylamine (pH 7.0; 0.3{\%} v/v in Milli-Q water) (60:40{\%}, v/v) at a flow rate of 1.0 mL min-1. This method was linear over the range of 50.0-3500.0 ng mL-1 with regression coefficient greater than 0.99. The mean recovery of aceclofenac and IS were 84.62 ± 3.23 and 89.19 ± 1.57{\%}, respectively and the method was found to be precise, accurate, and specific during the study. The method was successfully applied for pharmacokinetic study of aceclofenac in rats. {\circledC} 2006 Elsevier B.V. All rights reserved.",
author = "P. Musmade and G. Subramanian and K.K. Srinivasan",
note = "Cited By :37 Export Date: 10 November 2017 CODEN: ACACA Correspondence Address: Musmade, P.; Department of Pharmaceutical Quality Assurance, Manipal College of Pharmaceutical Sciences, Manipal, 576104, India; email: prashant.pqa@manipal.edu Chemicals/CAS: aceclofenac, 89796-99-6; ibuprofen, 15687-27-1; aceclofenac, 89796-99-6; Anti-Inflammatory Agents, Non-Steroidal; Diclofenac, 15307-86-5; Ethylamines; Ibuprofen, 15687-27-1; Methanol, 67-56-1; triethylamine, 121-44-8 Manufacturers: Karnataka, India; Sun, India References: Brogden, R.N., Wiseman, L.R., (1996) Drugs, 52, p. 113; Dooley, M., Spencer, C.M., Dunn, C.J., (2001) Drugs, 61, p. 1351; Martel-Pelletier, J., Cloutier, J.M., Pelletier, J.P., (1997) Clin. Drug Invest., 14, p. 226; Yamazaki, R., Kawai, S., Matsuzaki, T., Kaneda, N., Hashimoto, S., Yokokura, T., Okamoto, R., Mizushima, Y., (1999) Eur. J. Pharmacol., 28, p. 676; Legrand, E., (2004) Expert. Opin. Pharmacother., 5, p. 1347; (2001) Guidance for Industry: Bioanalytical Method Validation, , US Department of Health and Human Services, Food and Drug Administration, Center for Drug Evaluation and Research, Rockville, MD; Rimbau, V., Fernandez, M.F., Guirao, I., Torralba, A., Izquierdo, E., (1988) Farmaco [Prat], 43, p. 19; Lee, H.S., Jeong, C.K., Choi, S.J., Kim, S.B., Sohn, D.H., (2000) J. Pharm. Biomed. Anal., 23, p. 775; Zinellu, A., Carru, C., Sotgia, S., Porqueddu, E., Enrico, P., Deiana, L., (2005) Eur. J. Pharm. Sci., 24, p. 375; Jin, Y., Chen, H., Gu, S., Zeng, F., (2004) Se Pu, 22, p. 252; Hinz, B., Auge, D., Rau, T., Rietbrock, S., Brune, K., Werner, U., (2003) Biomed. Chromatogr., 17, p. 268; Liu, X.Q., Chen, X.J., Zhao, L.H., Peng, J.H., (1997) Yao Xue Xue Bao, 32, p. 546; Mitra, S., Sample preparation techniques in analytical chemistry (2003) Chemical Analysis, A Series of Monographs on Analytical Chemistry and its Applications, 165. , Wiley-Interscience, A John Wiley and sons, Inc. Publication p. 183",
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High-performance liquid chromatography and pharmacokinetics of aceclofenac in rats. / Musmade, P.; Subramanian, G.; Srinivasan, K.K.

In: Analytica Chimica Acta, Vol. 585, No. 1, 2007, p. 103-109.

Research output: Contribution to journalArticle

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AU - Subramanian, G.

AU - Srinivasan, K.K.

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Y1 - 2007

N2 - A simple and sensitive high-performance liquid chromatographic (HPLC) method was developed for quantification of aceclofenac in rat plasma. Ibuprofen was used as an internal standard (IS). The present method used protein precipitation for extraction of aceclofenac from rat plasma. Separation was carried out on reversed-phase C18 column (250 mm × 4.6 mm, 5 μ) and the column effluent was monitored by UV detector at 282 nm. The mobile phase used was methanol-triethylamine (pH 7.0; 0.3% v/v in Milli-Q water) (60:40%, v/v) at a flow rate of 1.0 mL min-1. This method was linear over the range of 50.0-3500.0 ng mL-1 with regression coefficient greater than 0.99. The mean recovery of aceclofenac and IS were 84.62 ± 3.23 and 89.19 ± 1.57%, respectively and the method was found to be precise, accurate, and specific during the study. The method was successfully applied for pharmacokinetic study of aceclofenac in rats. © 2006 Elsevier B.V. All rights reserved.

AB - A simple and sensitive high-performance liquid chromatographic (HPLC) method was developed for quantification of aceclofenac in rat plasma. Ibuprofen was used as an internal standard (IS). The present method used protein precipitation for extraction of aceclofenac from rat plasma. Separation was carried out on reversed-phase C18 column (250 mm × 4.6 mm, 5 μ) and the column effluent was monitored by UV detector at 282 nm. The mobile phase used was methanol-triethylamine (pH 7.0; 0.3% v/v in Milli-Q water) (60:40%, v/v) at a flow rate of 1.0 mL min-1. This method was linear over the range of 50.0-3500.0 ng mL-1 with regression coefficient greater than 0.99. The mean recovery of aceclofenac and IS were 84.62 ± 3.23 and 89.19 ± 1.57%, respectively and the method was found to be precise, accurate, and specific during the study. The method was successfully applied for pharmacokinetic study of aceclofenac in rats. © 2006 Elsevier B.V. All rights reserved.

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