Host genetic variations in glutathione-S-transferases, superoxide dismutases and catalase genes influence susceptibility to malaria infection in an Indian population

Rayzel C. Fernandes, Marriyah Hasan, Himanshu Gupta, K. Geetha, Padmalatha S. Rai, Manjunath H. Hande, Sydney C. D’Souza, Prabha Adhikari, Angela Brand, Kapaettu Satyamoorthy

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Antioxidant enzymes can contribute to disease susceptibility or determine response to therapy in individuals with malaria. Genetic variations due to polymorphisms in host genes encoding antioxidant enzymes such as glutathione S-transferases-theta, mu, pi (GSTT, GSTM, GSTP), superoxide dismutases (SOD) and catalase (CAT), may therefore, influence inter-individual response to malaria pathology and propensity of infection caused by Plasmodium vivax (Pv) and Plasmodium falciparum (Pf). Therefore, using polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) and DNA sequencing, we investigated the association of deletions of GSTT1 and GSTM1, single nucleotide polymorphisms (SNPs) of GSTP1 (rs1695), SOD1 (rs2234694), SOD2 (rs4880, rs1141718), SOD3 (rs2536512) and CAT (rs1001179) in individuals infected with Pf (n = 100) and Pv (n = 100) against healthy controls (n = 150). Our data suggest a significant role for GSTM1 deletions in complicated Pv (p = 0.0007) malaria with ODDs ratio 3.8 [with 95 % confidence interval (CI) 1.9–7.4]. The results also indicated that polymorphisms present in GSTP1, SOD1 and CAT genes may be associated with malaria susceptibility (p < 0.05), whereas SOD3 polymorphism may play a role in malarial resistance (p < 0.05). In addition, we observed significant SNP–SNP interactions with synergistic genetic effects in SOD2, SOD3 and CAT genes for Pv and in SOD2 and SOD3 genes for Pf. In conclusion, our results provide convincing evidence for a relationship between polymorphisms in host antioxidant enzymes and susceptibility to malaria infection.

Original languageEnglish
Pages (from-to)1155-1168
Number of pages14
JournalMolecular Genetics and Genomics
Volume290
Issue number3
DOIs
Publication statusPublished - 23-06-2015

Fingerprint

Plasmodium vivax
Glutathione Transferase
Catalase
Malaria
Superoxide Dismutase
Plasmodium falciparum
Infection
Antioxidants
Population
Genes
Enzymes
Disease Susceptibility
DNA Sequence Analysis
Single Nucleotide Polymorphism
Confidence Intervals
Pathology
Therapeutics

All Science Journal Classification (ASJC) codes

  • Medicine(all)
  • Molecular Biology
  • Genetics

Cite this

Fernandes, Rayzel C. ; Hasan, Marriyah ; Gupta, Himanshu ; Geetha, K. ; Rai, Padmalatha S. ; Hande, Manjunath H. ; D’Souza, Sydney C. ; Adhikari, Prabha ; Brand, Angela ; Satyamoorthy, Kapaettu. / Host genetic variations in glutathione-S-transferases, superoxide dismutases and catalase genes influence susceptibility to malaria infection in an Indian population. In: Molecular Genetics and Genomics. 2015 ; Vol. 290, No. 3. pp. 1155-1168.
@article{6697bebcff61456caf256f26fcfbd546,
title = "Host genetic variations in glutathione-S-transferases, superoxide dismutases and catalase genes influence susceptibility to malaria infection in an Indian population",
abstract = "Antioxidant enzymes can contribute to disease susceptibility or determine response to therapy in individuals with malaria. Genetic variations due to polymorphisms in host genes encoding antioxidant enzymes such as glutathione S-transferases-theta, mu, pi (GSTT, GSTM, GSTP), superoxide dismutases (SOD) and catalase (CAT), may therefore, influence inter-individual response to malaria pathology and propensity of infection caused by Plasmodium vivax (Pv) and Plasmodium falciparum (Pf). Therefore, using polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) and DNA sequencing, we investigated the association of deletions of GSTT1 and GSTM1, single nucleotide polymorphisms (SNPs) of GSTP1 (rs1695), SOD1 (rs2234694), SOD2 (rs4880, rs1141718), SOD3 (rs2536512) and CAT (rs1001179) in individuals infected with Pf (n = 100) and Pv (n = 100) against healthy controls (n = 150). Our data suggest a significant role for GSTM1 deletions in complicated Pv (p = 0.0007) malaria with ODDs ratio 3.8 [with 95 {\%} confidence interval (CI) 1.9–7.4]. The results also indicated that polymorphisms present in GSTP1, SOD1 and CAT genes may be associated with malaria susceptibility (p < 0.05), whereas SOD3 polymorphism may play a role in malarial resistance (p < 0.05). In addition, we observed significant SNP–SNP interactions with synergistic genetic effects in SOD2, SOD3 and CAT genes for Pv and in SOD2 and SOD3 genes for Pf. In conclusion, our results provide convincing evidence for a relationship between polymorphisms in host antioxidant enzymes and susceptibility to malaria infection.",
author = "Fernandes, {Rayzel C.} and Marriyah Hasan and Himanshu Gupta and K. Geetha and Rai, {Padmalatha S.} and Hande, {Manjunath H.} and D’Souza, {Sydney C.} and Prabha Adhikari and Angela Brand and Kapaettu Satyamoorthy",
year = "2015",
month = "6",
day = "23",
doi = "10.1007/s00438-014-0984-4",
language = "English",
volume = "290",
pages = "1155--1168",
journal = "Molecular Genetics and Genomics",
issn = "1617-4615",
publisher = "Springer Verlag",
number = "3",

}

Host genetic variations in glutathione-S-transferases, superoxide dismutases and catalase genes influence susceptibility to malaria infection in an Indian population. / Fernandes, Rayzel C.; Hasan, Marriyah; Gupta, Himanshu; Geetha, K.; Rai, Padmalatha S.; Hande, Manjunath H.; D’Souza, Sydney C.; Adhikari, Prabha; Brand, Angela; Satyamoorthy, Kapaettu.

In: Molecular Genetics and Genomics, Vol. 290, No. 3, 23.06.2015, p. 1155-1168.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Host genetic variations in glutathione-S-transferases, superoxide dismutases and catalase genes influence susceptibility to malaria infection in an Indian population

AU - Fernandes, Rayzel C.

AU - Hasan, Marriyah

AU - Gupta, Himanshu

AU - Geetha, K.

AU - Rai, Padmalatha S.

AU - Hande, Manjunath H.

AU - D’Souza, Sydney C.

AU - Adhikari, Prabha

AU - Brand, Angela

AU - Satyamoorthy, Kapaettu

PY - 2015/6/23

Y1 - 2015/6/23

N2 - Antioxidant enzymes can contribute to disease susceptibility or determine response to therapy in individuals with malaria. Genetic variations due to polymorphisms in host genes encoding antioxidant enzymes such as glutathione S-transferases-theta, mu, pi (GSTT, GSTM, GSTP), superoxide dismutases (SOD) and catalase (CAT), may therefore, influence inter-individual response to malaria pathology and propensity of infection caused by Plasmodium vivax (Pv) and Plasmodium falciparum (Pf). Therefore, using polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) and DNA sequencing, we investigated the association of deletions of GSTT1 and GSTM1, single nucleotide polymorphisms (SNPs) of GSTP1 (rs1695), SOD1 (rs2234694), SOD2 (rs4880, rs1141718), SOD3 (rs2536512) and CAT (rs1001179) in individuals infected with Pf (n = 100) and Pv (n = 100) against healthy controls (n = 150). Our data suggest a significant role for GSTM1 deletions in complicated Pv (p = 0.0007) malaria with ODDs ratio 3.8 [with 95 % confidence interval (CI) 1.9–7.4]. The results also indicated that polymorphisms present in GSTP1, SOD1 and CAT genes may be associated with malaria susceptibility (p < 0.05), whereas SOD3 polymorphism may play a role in malarial resistance (p < 0.05). In addition, we observed significant SNP–SNP interactions with synergistic genetic effects in SOD2, SOD3 and CAT genes for Pv and in SOD2 and SOD3 genes for Pf. In conclusion, our results provide convincing evidence for a relationship between polymorphisms in host antioxidant enzymes and susceptibility to malaria infection.

AB - Antioxidant enzymes can contribute to disease susceptibility or determine response to therapy in individuals with malaria. Genetic variations due to polymorphisms in host genes encoding antioxidant enzymes such as glutathione S-transferases-theta, mu, pi (GSTT, GSTM, GSTP), superoxide dismutases (SOD) and catalase (CAT), may therefore, influence inter-individual response to malaria pathology and propensity of infection caused by Plasmodium vivax (Pv) and Plasmodium falciparum (Pf). Therefore, using polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) and DNA sequencing, we investigated the association of deletions of GSTT1 and GSTM1, single nucleotide polymorphisms (SNPs) of GSTP1 (rs1695), SOD1 (rs2234694), SOD2 (rs4880, rs1141718), SOD3 (rs2536512) and CAT (rs1001179) in individuals infected with Pf (n = 100) and Pv (n = 100) against healthy controls (n = 150). Our data suggest a significant role for GSTM1 deletions in complicated Pv (p = 0.0007) malaria with ODDs ratio 3.8 [with 95 % confidence interval (CI) 1.9–7.4]. The results also indicated that polymorphisms present in GSTP1, SOD1 and CAT genes may be associated with malaria susceptibility (p < 0.05), whereas SOD3 polymorphism may play a role in malarial resistance (p < 0.05). In addition, we observed significant SNP–SNP interactions with synergistic genetic effects in SOD2, SOD3 and CAT genes for Pv and in SOD2 and SOD3 genes for Pf. In conclusion, our results provide convincing evidence for a relationship between polymorphisms in host antioxidant enzymes and susceptibility to malaria infection.

UR - http://www.scopus.com/inward/record.url?scp=84938861612&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84938861612&partnerID=8YFLogxK

U2 - 10.1007/s00438-014-0984-4

DO - 10.1007/s00438-014-0984-4

M3 - Article

VL - 290

SP - 1155

EP - 1168

JO - Molecular Genetics and Genomics

JF - Molecular Genetics and Genomics

SN - 1617-4615

IS - 3

ER -