HPLC-LIF for early detection of oral cancer

K. Venkatakrishna, V. B. Kartha, Keerthilatha M. Pai, C. Murali Krishna, O. Ravikiran, Jacob Kurian, Mohan Alexander, G. Ullas

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

At present, the diagnosis of many cancers relies on the subjective interpretation of morphological changes in biopsy samples. This usually provides only late diagnosis. Early detection, which can provide more successful therapy, is expected to be possible by identification of tumour markers in physiological samples. Immunoassay used at present for this purpose has several drawbacks. It is applicable only for known markers, can usually detect only one marker at a time, and may also fail to detect a marker when there exist conditions, which may mask or prevent the interaction between antigen and the antibody. We have developed a high performance liquid chromatography-laser induced fluorescence (HPLC-LIF) technique to detect and record simultaneously spectra and chromatograms of physiological samples, which will enable the detection of multiple 'markers' in a single physiological sample in a short time. Samples of saliva and serum from normal and oral cancer subjects have been studied with the set up. The present studies show that body fluids like saliva and serum of normal, pre-malignant and malignant subjects have substantially different protein profiles. By simultaneous recording of the chromatographic peaks and corresponding fluorescence spectra, it is possible to carry out unambiguous discrimination between normal, pre-malignant and malignant cases even when markers are present in femto/subfemtomole quantities, which should assist in early diagnosis of neoplasia.

Original languageEnglish
Pages (from-to)551-557
Number of pages7
JournalCurrent Science
Volume84
Issue number4
Publication statusPublished - 25-02-2003

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Mouth Neoplasms
Early Detection of Cancer
Lasers
Fluorescence
High Pressure Liquid Chromatography
Saliva
Delayed Diagnosis
Body Fluids
Tumor Biomarkers
Masks
Serum
Immunoassay
Early Diagnosis
Neoplasms
Biopsy
Antigens
Antibodies
Proteins
Therapeutics

All Science Journal Classification (ASJC) codes

  • General

Cite this

Venkatakrishna, K., Kartha, V. B., Pai, K. M., Krishna, C. M., Ravikiran, O., Kurian, J., ... Ullas, G. (2003). HPLC-LIF for early detection of oral cancer. Current Science, 84(4), 551-557.
Venkatakrishna, K. ; Kartha, V. B. ; Pai, Keerthilatha M. ; Krishna, C. Murali ; Ravikiran, O. ; Kurian, Jacob ; Alexander, Mohan ; Ullas, G. / HPLC-LIF for early detection of oral cancer. In: Current Science. 2003 ; Vol. 84, No. 4. pp. 551-557.
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Venkatakrishna, K, Kartha, VB, Pai, KM, Krishna, CM, Ravikiran, O, Kurian, J, Alexander, M & Ullas, G 2003, 'HPLC-LIF for early detection of oral cancer', Current Science, vol. 84, no. 4, pp. 551-557.

HPLC-LIF for early detection of oral cancer. / Venkatakrishna, K.; Kartha, V. B.; Pai, Keerthilatha M.; Krishna, C. Murali; Ravikiran, O.; Kurian, Jacob; Alexander, Mohan; Ullas, G.

In: Current Science, Vol. 84, No. 4, 25.02.2003, p. 551-557.

Research output: Contribution to journalArticle

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Venkatakrishna K, Kartha VB, Pai KM, Krishna CM, Ravikiran O, Kurian J et al. HPLC-LIF for early detection of oral cancer. Current Science. 2003 Feb 25;84(4):551-557.